Microbial food cultures
Microbial food cultures are live bacteria, yeasts or moulds used in food production. Microbial food cultures carry out the fermentation process in foodstuffs. Used by humans since the Neolithic period (around 10 000 years BC) fermentation helps to preserve perishable foods and to improve their nutritional and organoleptic qualities (in this case, taste, sight, smell, touch). As of 1995, fermented food represented between one quarter and one third of food consumed in Central Europe. More than 260 different species of microbial food culture are identified and described for their beneficial use in fermented food products globally, showing the importance of their use.
The scientific rationale of the function of microbes in fermentation started to be built with the discoveries of Louis Pasteur in the second half of the 19th century. Extensive scientific study continues to characterize microbial food cultures traditionally used in food fermentation taxonomically, physiologically, biochemically and genetically. This allows better understanding and improvement of traditional food processing and opens up new fields of applications.
Microorganisms are the earliest form of life on earth, first evolving more than three billion years ago. Our ancestors discovered how to harness the power of microorganisms to make new foods, even if they did not know the science behind what they were doing.
Starting from 1970s—production of first industrial concentrated cultures, frozen or freeze-dried cultures, for the direct inoculation of processed milk, improving the regularity of production processes.
Function of microbial food cultures in food
Microbial food cultures preserve food through formation of inhibitory metabolites such as organic acid (lactic acid, acetic acid, formic acid, propionic acid), ethanol, bacteriocins, etc., often in combination with decrease of water activity (by drying or use of salt). Further, microbial food cultures help to improve food safety through inhibition of pathogens or removing of toxic compounds. Microbial food cultures also improve the nutritional value and organoleptic quality of the food.
Starter cultures have mainly a technological function in the food manufacturing. They are used as food ingredients at one or more stages in the food manufacturing process and develop the desired metabolic activity during the fermentation or ripening process. They contribute to the one or multiple unique properties of a foodstuff especially in regard to taste, flavour, colour, texture, safety, preservation, nutritional value, wholesomeness and/or health benefits.
Generally, the bacteria used as starter culture are not the same used as probiotics. There are, however, cases when one bacterium can be used both as starter culture and as probiotic. The scientific community is presently trying to deepen understanding of the roles played by microbes in food processing and human health.
Bacterial food cultures are responsible for the aroma, taste and texture of cheeses and fermented milk products such as yogurts, ayran, doogh, skyr or ymer. They contribute to developing the flavour and colour of such fermented products as salami, pepperoni and dried ham. Lactic acid bacteria converts the unstable malic acid that is naturally present in wine into the stable lactic acid. This malolactic fermentation gives the stability that is characteristic of high-quality wines that improve on storage.
S. cerevisiae feeds on the sugars present in the bread dough and produces the gas carbon dioxide. This forms bubbles within the dough, causing it to expand and the bread to rise.
Several different yeasts are used in brewing beer, where they ferment the sugars present in malted barley to produce alcohol. One of the most common is S. cerevisiae. The same strain of S. cerevisiae which can also be used in breadmaking is used to make ale-type beers. It is known as a top-fermenting yeast because it creates a foam on the top of the brew. Bottom-fermenting yeasts, such as S. pastorianus, are more commonly used to make lagers. They ferment more of the sugars in the mixture than top-fermenting yeasts, which gives a cleaner taste.
The alcohol in wine is formed by the fermentation of the sugars in grape juice, with carbon dioxide as a by-product. Yeast is naturally present on grapeskins, and this alone can be sufficient for the fermentation of sugars to alcohol to occur. A pure yeast culture, most often S. cerevisiae, is usually added to ensure the fermentation is reliable. Other yeast cultures like Pichia, Torulaspora and Kluyveromyces are naturally present or added to create special flavours in the wine. Sparkling wine, including champagne, is made by adding further yeast to the wine when it is bottled. The carbon dioxide formed in this second fermentation is trapped as bubbles.
To make blue cheese, the cheese is treated with a mould, usually Penicillium roqueforti, while it is still in the loosely pressed curd form. As the cheese matures, the mould grows, creating blue veins within it which gives the cheese its characteristic flavour. Examples include stilton, roquefort and gorgonzola.
Soft ripened cheese such as brie and camembert are made by allowing P. camemberti to grow on the outside of the cheese, which causes them to age from the outside in. The mould forms a soft white crust, and the interior becomes runny with a strong flavour.
Rind-washed cheeses like limburger also ripen inwards, but here, as the name suggests, they are washed with brine and other ingredients such as beer and wine which contain mould. This also makes them attractive to bacteria, which add to the flavour.
Traditionally, inoculations of sausages with moulds were done with the indigenous biota of the slaughters. Different moulds (such as P. chrysogenum and P. nalgiovense) can be used to ripen surfaces of sausages. The mould cultures develop the aroma and improve the texture of the sausages. They also contribute to shortening of the ripening period and preserving the natural quality. This expands the shelf life of the meat product.
In the past, soy sauce has been made by mixing soybeans and other grains with a mould (Aspergillus oryzae or A. sojae) and yeast. This mixture was then left to ferment in the sun. Today soy sauce is made under controlled conditions. The key flavour ingredients formed in this process are salts of the amino acid glutamic acid, notably monosodium glutamate.
Production of microbial food cultures
The industrial production of microbial food cultures is carried out after careful selection process and under strictly controlled conditions. First, the microbiology laboratory, where the original strains are kept, prepares the inoculation material, which is a small quantity of microbes of a single (pure) strain. Then, the inoculation material is multiplied and grown either in fermenters (liquid) or on a surface (solid) under defined and monitored conditions. Grown cells of pure culture are harvested, eventually blended with other cultures and, finally, formulated (preserved) for subsequent transportation and storage. They are sold in liquid, frozen or freeze-dried formats.
Another and traditional way of starting a food fermentation is often referred to as spontaneous fermentation. Cultures come from raw milk, i.e. milk that has not undergone any sanitation treatment or from the reuse of a fraction of the previous production (back-slopping). The composition of such cultures is complex and extremely variable. The use of such techniques is steadily decreasing in developed countries. Some countries even prohibit the back-slopping technique because of the "potential to magnify pathogen loads to very dangerous levels".
Safety and regulatory aspects
Microbial food cultures are considered as traditional food ingredients and are permitted in the production of foodstuffs all over the world under general food laws.
Commercially available microbial food cultures are sold as preparations, which are formulations, consisting of concentrates of one or more microbial species and/or strains including unavoidable media components carried over from the fermentation and components, which are necessary for their survival, storage, standardisation and to facilitate their application in the food production process.
Safety of microbial food cultures, depending on their characteristics and use, can be based on genus, species or strain levels.
Microorganisms with documented history of safe use in food
The first (non-exhaustive) inventory of microorganisms with a documented history of use in food was for the first time compiled in 2001 by the International Dairy Federation (IDF) and the European Food and Feed Cultures Association (EFFCA).
In the United States of America, microbial food cultures are regulated under the Food, Drug and Cosmetic Act. Section 409 of the 1958 Food Additives Amendment of the Food, Drug and Cosmetic Act, exempts from the definition of food additives substances generally recognized by experts as safe (GRAS) under conditions of their intended use. These substances do not require premarket approval by the US Food and Drug Administration.
Since 2007, the European Food Safety Authority (EFSA) has been maintaining a list of microorganisms having qualified presumption of safety (QPS). The QPS list covers only a limited number of microorganisms, which have been referred to EFSA for safety assessment. It has been conceived as an internal evaluation tool for microorganisms used in the food production chain (e.g. feed cultures, cell factories producing enzymes or additives, plant protection) that need an evaluation by EFSA scientific panels before being marketed in the EU. Microbial food cultures with a long history of safe use are, however, considered to be traditional food ingredients and are legally permitted for use in human food without EFSA evaluation.
From 1974 to 2010 Denmark required premarket approval of microbial food cultures. The positive list of microbial food cultures is available on the website of the Danish Veterinary and Food Administration.
In 2010, the regulation changed. Approval is no longer needed but a notification should be made to the Veterinary and Food Administration.
- Prajapati, J.B., Nair, B.M. (2003). Farnworth, E.R., ed. The history of fermented foods in Fermented Functional Foods. CRC Press, Boca Raton, New York, London, Washington DC. pp. 1–25.
- Holzapfel, W.H., U. Schillinger, and R. Geisen (January 1995). "Biological Preservation of Foods with Reference to Protective Cultures, Bacteriocins and Food-Grade Enzymes". International Journal of Food Microbiology. 24 (3): 343–362. doi:10.1016/0168-1605(94)00036-6.
- Bourdichon, F., Casaregola, S., Farrokh, C., Frisvad, J.C., Gerds, M.L., Hammes, W.P., Harnett, J., Huys, G., Laulund, S., Ouwehand, A., Powell, I.B., Prajapati, J.B., Seto, Y., Ter Schure, E., Van Boven, A., Vankerckhoven, V., Zgoda, A., Tuijtelaars, S., Bech Hansen, E. (2012). "Food fermentations: Microorganisms with technological beneficial use". International Journal of Food Microbiology. 154: 87–97. doi:10.1016/j.ijfoodmicro.2011.12.030.
- Wyman, J. (1862). "Spontaneous generation". British Medical Journal. 2 (90): 311–312. doi:10.1136/bmj.2.90.311. PMC . PMID 20744126.
- Farley, J., Geison, G.L., J; Geison, GL (1974). "Science, politics and spontaneous generation in nineteenth-century France: the Pasteur-Pouchet debate". Bulletin of the History of Medicine. 48 (2): 161–198. PMID 4617616.
- Schopf J (2006). "Fossil evidence of Archaean life" (PDF). Philos Trans R Soc Lond B Biol Sci. 361 (1470): 869–85. doi:10.1098/rstb.2006.1834. PMC . PMID 16754604.
- Altermann W, Kazmierczak J (2003). "Archean microfossils: a reappraisal of early life on Earth". Res Microbiol. 154 (9): 611–7. doi:10.1016/j.resmic.2003.08.006. PMID 14596897.
- Cavalier-Smith T (2006). "Cell evolution and Earth history: stasis and revolution" (PDF). Philos Trans R Soc Lond B Biol Sci. 361 (1470): 969–1006. doi:10.1098/rstb.2006.1842. PMC . PMID 16754610.
- McGovern, P. E.; Zhang, J.; Tang, J.; Zhang, Z.; Hall, G. R.; Moreau, R. A.; Nunez, A.; Butrym, E. D.; Richards, M. P.; Wang, C. -S.; Cheng, G.; Zhao, Z.; Wang, C. (2004). "Fermented beverages of pre- and proto-historic China". Proceedings of the National Academy of Sciences. 101 (51): 17593–17598. doi:10.1073/pnas.0407921102. PMC . PMID 15590771.
- "8,000-year-old wine unearthed in Georgia". The Independent. 2003-12-28. Retrieved 2007-01-28.
- "Now on display ... world's oldest known wine jar". Retrieved 2007-01-28.
- "Fermented fruits and vegetables. A global perspective". FAO Agricultural Services Bulletins - 134. Archived from the original on January 19, 2007. Retrieved 2007-01-28.
- Cavalieri, D; McGovern P.E.; Hartl D.L.; Mortimer R.; Polsinelli M. (2003). "Evidence for S. cerevisiae fermentation in ancient wine." (PDF). Journal of Molecular Evolution. 57 Suppl 1: S226–32. doi:10.1007/s00239-003-0031-2. PMID 15008419. 15008419. Archived from the original (PDF) on December 9, 2006. Retrieved 2007-01-28.
- Gest, H., H (2004). "The discovery of microorganisms by Robert Hooke and Antoni van Leeuwenhoek, Fellows of The Royal Society". Notes and Records of the Royal Society of London. 58 (2): 187–201. doi:10.1098/rsnr.2004.0055. PMID 15209075.
- John Long Wilson. "Stanford University School of Medicine and the Predecessor Schools: An Historical Perspective". Retrieved 8 August 2012.
- "Emile Christian Hansen". Encyclopædia Britannica.
- Jespersen, L., Josephsen, J. (2004). Hui, Y.H.; et al., eds. Starter Cultures and Fermented Products in Handbook of Food and Beverage Fermentation Technology. CRC Press. ISBN 978-0-8247-4780-0.
- "La biodiversité des microorganismes des produits laitiers". Les microorganismes bénéfiques de l’industrie de transformation du lait. INRA. Retrieved 8 August 2012.
- "Sigurd Orla-Jensen". Biokemisk Forening.
- Ross, R.P., Morgan, S., Hill, C. (2002). Preservation and fermentation: past, present and future. International Journal of Food Microbiology. 79: 3–16. Missing or empty
- Gaggia, F., Di Gioia, D., Baffoni, L., Biavati, B. (2011). "The role of protective and probiotics cultures in food and feed and their impact on food safety". Trends in Food Science and Technology. 22: S58–S66. doi:10.1016/j.tifs.2011.03.003.
- Adams, M., Mitchell, R. (2002). "Fermentation and pathogen control: a risk assessment approach". International Journal of Food Microbiology. 79: 75–83. doi:10.1016/s0168-1605(02)00181-2.
- Adams, M.R., Nicolaides, L. (2008). "Review of the sensitivity of different foodborne pathogens to fermentation". Food Control. 8: 227–239. doi:10.1016/s0956-7135(97)00016-9.
- Hammes, W.P., Tichaczek, P.S. (1994). "The potential of lactic acid bacteria for the production of safe and wholesome food". Zeitschrift für Lebensmittel-Untersuchung und -Forschung. 198: 193–201. doi:10.1007/bf01192595.
- van Boekel, M., Fogliano, V., Pellegrini, N., Stanton, C., Scholz, G., Lalljie, S., Somoza, V., Knorr, D., Jasti, P.R., Eisenbrand, G. (2010). "A review on the beneficial aspects of food processing". Molecular Nutrition & Food Research. 54: 1215–1247. doi:10.1002/mnfr.200900608.
- Poutanen, K., Flander, L., Katina, K. (2009). "Sourdough and cereal fermentation in an nutritional perspective". Food Microbiology. 7: 693–699. doi:10.1016/j.fm.2009.07.011.
- Marilley, L., Casey, M.G. (2004). "Flavors of cheese products: metabolic pathways, analytical tools and identification of producing strains". International Journal of Food Microbiology. 90: 139–159. doi:10.1016/s0168-1605(03)00304-0.
- Smit, G., Smit, B.A., Engels, W.J. (2005). "Flavor formation by lactic acid bacteria and biochemical flavor profiling of cheese products". FEMS Microbiology Reviews. 29: 591–610. doi:10.1016/j.femsre.2005.04.002.
- Lacroix, N., St Gelais, D., Champagne, C.P., Fortin, J., Vuillemard, J.C. (2010). "Characterization of aromatic properties of old-style cheese starters". Journal of Dairy Science. 93: 3427–3441. doi:10.3168/jds.2009-2795.
- Sicard, D., Legras, J.L. (2011). "Bread, beer and wine: yeast domestication in the Saccharomyces sensu strict complex". Comptes Rendus Biologies. 334 (3): 229–236. doi:10.1016/j.crvi.2010.12.016.
- "Raw-fermented sausages". Food and Agriculture Organization of the United Nations (FAO). Retrieved 8 August 2012.
- Wu, Z.-Y., Zhang, W.-X., Zhang, Q.-S., Hu, C., Wang, R., Liu, Z.-H. (2009). "Developing New Sacchariferous Starters for Liquor Production Based on Functional Strains Isolated from the Pits of Several Famous Luzhou-flavor Liquor Brewers". Journal of the institute of brewing. 115 (2): 111–115. doi:10.1002/j.2050-0416.2009.tb00354.x.
- Mogra, R., Choudhry, M. (2008). "Effect of starter culture on the development of curd". J. Dairying, Foods & H.S. 27 (2): 130–133.
- "Health and Nutritional Properties of Probiotics in Food including Powder Milk with Live Lactic Acid Bacteria" (PDF). Report of a Joint FAO/WHO Expert Consultation on Evaluation of Health and Nutritional Properties of Probiotics in Food Including Powder Milk with Live Lactic Acid Bacteria. World Health Organisation of the United nations (WHO). Retrieved 8 August 2012.
- Rijkers, G.T., de Vos, W.M., Brummer, R.-J., Morelli, L., Corthier, G., Marteau, P. (2011). "Health benefits and health claims of probiotics: bridging science and marketing". British Journal of Nutrition: 1291–6. doi:10.1017/s000711451100287x.
- Heller, K.J. "Probiotic bacteria in fermented foods: product characteristics and starter organisms". American Society for Clinical Nutrition. Retrieved 8 August 2012.
- Vinderola, C.G., Reinheimer, J.A. (2003). "actic acid starter and probiotic bacteria: a comparative "in vitro" study of probiotic characteristics and biological barrier resistance". Food Research International. 36 (9-10): 895–904. doi:10.1016/s0963-9969(03)00098-x.
- Szabo, L. (updated 5/8/2012). "New research on probiotics shows promise". USA Today. Retrieved 8 August 2012. Check date values in:
- "Human Microbiome Project". Retrieved 8 Mar 2012.
- "Fermented frutis and vegetables. A global perspective". Chapter 5 - Bacterial fermentations. Food and Agriculture Organization of the United Nations (FAO). Retrieved 8 August 2012.
- Ribéreau-Gayon, P., Dubourdieu, D., Donèche, B., Lonvaud, A. (2006). Handbook of Enology, Volume 1: The microbiology of wine and vinifications. J.Wiley & Sond Ltd., Chichester, U.K.
- Lonvaud-Funel, A. (1999). "Lactic acid bacteria in the quality improvement and depreciation of wine". Antonie van Leeuwenhoek. 76: 317–331. doi:10.1007/978-94-017-2027-4_16.
- Gueimonde, M., de Los Reyes- Gavilán, C.G., Borja Sánchez, B. (2011). Von Wright, A., ed. Lactic Acid Bacteria, Microbiological and Functional Aspects, Fourth Edition. CRC Press. ISBN 978-1-4398-3677-4.
- Yabaya, A., Jatau, E.D. (2009). "Investigating Wild Yeast Baking Potentials". Middle-East Journal of Scientific Research. 4 (4): 320–322.
- "Biochemistry of yeast fermentation" (PDF). University of British Columbia. Retrieved 8 August 2012.
- Libkinda, D., Hittingerb, C.T., Valériod, E., Gonçalvesd, C., Doverb, J., Johnstonb, M., Gonçalvesd, P., Sampaiod, J.P. "Microbe domestication and the identification of the wild genetic stock of lager-brewing yeast". Proceedings of the National Academy of Sciences of the United States of America. Retrieved 8 August 2012.
- Mills, D.A., Phister, T., Neeley, E., Johannsen, E. (2008). Cocolin, L., Ercolini, D., ed. Wine Fermentation in Molecular Techniques in the Microbial Ecology of Fermented Foods. Springer.
- McCarthy, E., Ewing-Mulligan, M. "The Methods of Producing Sparkling Wine". John Wiley & Sons, Inc. Retrieved 8 August 2012.
- "Kefir, The champagne of Dairy Products". Armand-Frappier Museum.
- Boekhout, T., Robert, V. (2003). Yeasts in food. Woodhead. ISBN 9781855737068.
- Nelson, J.H. (1970). "Production of Blue cheese flavor via submerged fermentation by Penicillium roqueforti". Journal of Agricultural and Food Chemistry. 18 (4): 567–569. doi:10.1021/jf60170a024.
- Leclercq-Perlat, M.N.; et al. (2004). "Controlled production of Camembert-type cheeses.". Journal of Dairy Research. 71 (3).
- "Washed Rind Cheese". Dairy Australia. Retrieved 9 August 2012.
- Ludemann, V., Pose, G., Moavro, A., Maliaviabarrena, M.G., Fandiño, F., Ripoll, G., Basílico, J.C., Pardo, A.G. (September 2009). "Toxicological assessment of Penicillium nalgiovense strains for use as starter cultures in the manufacture of dry fermented sausages". Journal of food protection. 72 (8): 1666–70.
- Marianski, S., Mariański, A. (2009). The Art of Making Fermented Sausages. Bookmagic LLC.
- Lund, B., Baird-Parker, A.C., Gould, G.W. (2000). Microbiological Safety and Quality of Food. Aspen Publishers Inc.
- Machida, M., Yamada, O., Gomi, K. (2008). "Genomics of Aspergillus oryzae: Learning from the History of Koji Mold and Exploration of Its Future". DNA Research. 15: 173–183. doi:10.1093/dnares/dsn020.
- "MSG". New South Wales (Australia) Food Authority.
- Gaggia, F., Di Gioia, D., Baffoni, L., Biavati, B. (2011). "The role of protective and probiotic cultures in food and feed and their impact in food safety". Trends in Food Science & Technology. 22 (1): S58–S66. doi:10.1016/j.tifs.2011.03.003.
- "Microbiology of starter cultures". Dairy Science Food Technology. Retrieved 9 August 2012.
- Varnam, A., Sutherland,J.M. (1995). Meat and Meat Products: Technology, Chemistry and Microbiology. Chapman & Hall, UK.
- Ross, T., Shadbolt, C.T. "Predicting Escherichia coli inactivation in uncooked comminuted fermented meat products, Final Report" (PDF). Meat & Livestock Australia.
- Health Canada, 2003. Amendment (Schedule No. 948) to Division 28 of the Food and Drug Regulations, Sections B.28.001–003. Food and Drugs Regulations
- Mogensen, G., Salminen, S., O'Brien, J., Ouwehand, A., Holzapfel, W., Shortt, C., Fonden, R., Miller, G.D., Donohue, D., Playne, M., Crittenden, R., Salvadori, B., Zink, R. (2002). "Inventory of microorganisms with a documented history of use in food". Bulletin of IDF. 377: 10–19.
- "Federal Food, Drug, and Cosmetic Act (FD&C Act)". US Food and Drug Administration. Retrieved 10 August 2012.
- "How U.S. FDA's GRAS Notification Program Works". US Food and Drug Administration. Retrieved 10 August 2012.
- Stevens, H., O'Brien Nabors, L. (2009). "Microbial Food Cultures:a regulatory update" (PDF). Food Technology: 36–41.
- "Regulation (EC) no 178/2002 of the European Parliament and of the Council of 28 January 2002 laying down the general principles and requirements of food law, establishing the European Food Safety Authority and laying down procedures in matters of food safety". OJ EC. L31/1. 2002.
- Herody, C., Soyeux, Y., Bech Hansen, E., Gillies, K. (2010). "The legal status of microbial food cultures in the European Union: an overview". European Food and Feed Law Review. 5: 258–269.
- "Qualified presumption of safety (QPS)". European Food Safety Authority. Retrieved 10 August 2012.
- Anon, 2005. EFSA Scientific Colloquium—Microorganisms in Food and Feed:Qualified Presumption of Safety — 13–14 December 2004, Brussels, Belgium. ISSN 1830-4737, http://www.efsa.europa.eu/en/supporting/pub/109e.htm.
- Leuschner, R.-G.K., Robinson, T., Hugas, M., Cocconcelli, P.S., Richard-Forget, F., Klein, G., Licht, T.R., Nguyen-The, C., Querol, A., Richardson, M., Suarez, J.E., Vlak, J.M., von Wright, A. (2010). "Qualified presumption of safety (QPS): a generic risk assessment approach for biological agents notified to the European Food Safety Authority (EFSA)". Trends in Food Science and Technology. 21: 425–435. doi:10.1016/j.tifs.2010.07.003.
- "Liste over anmeldte mikrobielle kulturer" (PDF). Ministeriet for Fødevarer, Landbrug og Fiskeri. Retrieved 10 August 2012.
- "Anmeldelsesordning for kulturer af bakterier samt gær- og skimmelsvampe". Ministeriet for Fødevarer, Landbrug og Fiskeri.