Born in Santo André, São Paulo, Milena Toscano started her career as a model when she was ten years-old, after she was hired by the Ford Models Agency. She performed in her first TV commercial when she was 13 years old. After attending many performing arts schools such as Studio Fátima Toledo, Casa de Artes de Laranjeiras and Escola Livre de Teatro, she was signed by Rede Globo. She debuted in the 2004 telenovela Começar de Novo, playing the character Sofia. Milena Toscano played Martina in SBT's remake of the Mexican telenovela Los Ricos También Lloran, named Los Ricos também Choram, which aired from 2005 to 2006. She also played a lead role in 2010, in the Rede Globo telenovela Araguaia, playing Manuela Martinez. She played the character Vanessa in the 2011 telenovela Fina Estampa. Milena Toscano played an abolitionist and feminist named Filipa do Amaral in the 2016 Escrava Mãe.