Molecular weight cut-off or MWCO refers to the lowest molecular weight solute (in daltons) in which 90% of the solute is retained by the membrane, or the molecular weight of the molecule (e.g. globular protein) that is 90% retained by the membrane.
Microdialysis may also be used to separate nanoparticles from the solutions in which they were formed. In such a separation, the eluate will consist of non-complexed reactants and components.
Ultrafiltration membrane manufacturers commonly produce and offer MWCO's of 2k, 5k, 10k, 30k, 50k, 100k, and 1,000k. Devices offered range from laboratory focused centrifugal devices (100ul to 100ml) to laboratory and bioprocessing relevant tangential flow filtration (TFF) devices (50ml to hundreds of litres).
- "Technical Resource Library from". Cole-Parmer. 2006-08-25. Retrieved 2013-06-20.