A Morpholino, also known as a Morpholino oligomer and as a phosphorodiamidate Morpholino oligomer (PMO), is a type of oligomer molecule (colloquially, an oligo) used in molecular biology to modify gene expression. Its molecular structure has DNA bases attached to a backbone of methylenemorpholine rings linked through phosphorodiamidate groups. Morpholinos block access of other molecules to small (~25 base) specific sequences of the base-pairing surfaces of ribonucleic acid (RNA). Morpholinos are used as research tools for reverse genetics by knocking down gene function.
This article discusses only the Morpholino antisense oligomers, which are nucleic acid analogs. The word "Morpholino" can occur in other chemical names, referring to chemicals containing a six-membered morpholine ring. To help avoid confusion with other morpholine-containing molecules, when describing oligos "Morpholino" is often capitalized as a trade name, but this usage is not consistent across scientific literature.
Gene knockdown is achieved by preventing cells from making a targeted protein. Knocking down gene expression is a method for learning about the function of a particular protein; in a similar manner, causing a specific exon to be spliced out of the RNA transcript encoding a protein can help to determine the function of the protein moiety encoded by that exon or can sometimes knock down the protein activity altogether. These molecules have been applied to studies in several model organisms, including mice, zebrafish, frogs and sea urchins. Morpholinos can also modify the splicing of pre-mRNA or inhibit the maturation and activity of miRNA. Techniques for targeting Morpholinos to RNAs and delivering Morpholinos into cells have recently been reviewed in a journal article  and in book form.
Morpholinos are in development as pharmaceutical therapeutics targeted against pathogenic organisms such as bacteria or viruses and genetic diseases. The Morpholino drug eteplirsen from Sarepta Therapeutics received accelerated approval from the US Food and Drug Administration for treatment of some mutations causing Duchenne muscular dystrophy.
- 1 History
- 2 Structure
- 3 Function
- 4 Specificity, stability and non-antisense effects
- 5 Delivery
- 6 See also
- 7 References
- 8 Further reading
Morpholinos are synthetic molecules that are the product of a redesign of natural nucleic acid structure. Usually 25 bases in length, they bind to complementary sequences of RNA or single-stranded DNA by standard nucleic acid base-pairing. In terms of structure, the difference between Morpholinos and DNA is that, while Morpholinos have standard nucleic acid bases, those bases are bound to morpholine rings linked through phosphorodiamidate groups instead of phosphates. The figure compares the structures of the two strands depicted there, one of RNA and the other of a Morpholino. Replacement of anionic phosphates with the uncharged phosphorodiamidate groups eliminates ionization in the usual physiological pH range, so Morpholinos in organisms or cells are uncharged molecules. The entire backbone of a Morpholino is made from these modified subunits.
Morpholinos do not degrade their target RNA molecules, unlike many antisense structural types (e.g., phosphorothioates, siRNA). Instead, Morpholinos act by "steric blocking", binding to a target sequence within an RNA, inhibiting molecules that might otherwise interact with the RNA. Morpholino oligos are often used to investigate the role of a specific mRNA transcript in an embryo. Developmental biologists inject Morpholino oligos into eggs or embryos of zebrafish, African clawed frog (Xenopus), sea urchin and killifish (F. heteroclitus) producing morphant embryos, or electroporate Morpholinos into chick embryos at later development stages. With appropriate cytosolic delivery systems, Morpholinos are effective in cell culture. Vivo-Morpholinos, in which the oligo is covalently linked to a delivery dendrimer, enter cells when administered systemically in adult animals.
Normal gene expression in eukaryotes
In eukaryotic organisms, pre-mRNA is transcribed in the nucleus, introns are spliced out, then the mature mRNA is exported from the nucleus to the cytoplasm. The small subunit of the ribosome usually starts by binding to one end of the mRNA and is joined there by various other eukaryotic initiation factors, forming the initiation complex. The initiation complex scans along the mRNA strand until it reaches a start codon, and then the large subunit of the ribosome attaches to the small subunit and translation of a protein begins. This entire process is referred to as gene expression; it is the process by which the information in a gene, encoded as a sequence of bases in DNA, is converted into the structure of a protein. A Morpholino can modify splicing or block translation, depending on the Morpholino's base sequence.
Bound to the 5'-untranslated region of messenger RNA (mRNA), Morpholinos can interfere with progression of the ribosomal initiation complex from the 5' cap to the start codon. This prevents translation of the coding region of the targeted transcript (called "knocking down" gene expression). This is useful experimentally when an investigator wishes to know the function of a particular protein; Morpholinos provide a convenient means of knocking down expression of the protein and learning how that knockdown changes the cells or organism. Some Morpholinos knock down expression so effectively that, after degradation of preexisting proteins, the targeted proteins become undetectable by Western blot.
In 2016 a synthetic peptide-conjugated PMO (PPMO) was found to inhibit the expression of New Delhi Metallo-beta-lactamase, an enzyme that many drug-resistant bacteria use to destroy carbapenems.
Modifying pre-mRNA splicing
Morpholinos can interfere with pre-mRNA processing steps either by preventing splice-directing small nuclear ribonucleoproteins (snRNP) complexes from binding to their targets at the borders of introns on a strand of pre-mRNA, or by blocking the nucleophilic adenine base and preventing it from forming the splice lariat structure, or by interfering with the binding of splice regulatory proteins such as splice silencers and splice enhancers. Preventing the binding of snRNP U1 (at the donor site) or U2/U5 (at the polypyrimidine moiety and acceptor site) can cause modified splicing, commonly excluding exons from the mature mRNA. Targeting some splice targets results in intron inclusions, while activation of cryptic splice sites can lead to partial inclusions or exclusions. Targets of U11/U12 snRNPs can also be blocked. Splice modification can be conveniently assayed by reverse-transcriptase polymerase chain reaction (RT-PCR) and is seen as a band shift after gel electrophoresis of RT-PCR products.
Other applications: blocking other mRNA sites and use as probes
Morpholinos have been used to block miRNA activity and maturation. Fluorescein-tagged Morpholinos combined with fluorescein-specific antibodies can be used as probes for in-situ hybridization to miRNAs. Morpholinos can block ribozyme activity. U2 and U12 snRNP functions have been inhibited by Morpholinos. Morpholinos targeted to "slippery" mRNA sequences within protein coding regions can induce translational frameshifts. Morpholinos can block RNA editing. Morpholino activities against this variety of targets suggest that Morpholinos can be used as a general-purpose tool for blocking interactions of proteins or nucleic acids with mRNA.
Specificity, stability and non-antisense effects
Morpholinos have become a standard knockdown tool in animal embryonic systems, which have a broader range of gene expression than adult cells and can be strongly affected by an off-target interaction. Following initial injections into frog or fish embryos at the single-cell or few-cell stages, Morpholino effects can be measured up to five days later, after most of the processes of organogenesis and differentiation are past, with observed phenotypes consistent with target-gene knockdown. Control oligos with irrelevant sequences usually produce no change in embryonic phenotype, evidence of the Morpholino oligo's sequence-specificity and lack of non-antisense effects. The dose required for a knockdown can be reduced by coinjection of several Morpholino oligos targeting the same mRNA, which is an effective strategy for reducing or eliminating dose-dependent off-target RNA interactions.
MRNA rescue experiments can often restore the wild-type phenotype to the embryos and provide evidence for the specificity of a Morpholino. In an mRNA rescue, a Morpholino is co-injected with an mRNA that codes for the morphlino's protein. However, the rescue mRNA has a modified 5'-UTR (untranslated region) so that the rescue mRNA contains no target for the Morpholino. The rescue mRNA's coding region encodes the protein of interest. Translation of the rescue mRNA replaces production of the protein that was knocked down by the Morpholino. Since the rescue mRNA would not affect phenotypic changes due to the Morpholino's off-target gene expression modulation, this return to wild-type phenotype is further evidence of Morpholino specificity.
Because of their completely unnatural backbones, Morpholinos are not recognized by cellular proteins. Nucleases do not degrade Morpholinos, nor are they degraded in serum or in cells. Morpholinos do not activate toll-like receptors or innate immune responses such as interferon induction or the NF-κB-mediated inflammation response. Morpholinos are not known to modify DNA methylation.
Up to 18% of Morpholinos appear to induce nontarget-related phenotypes including cell death in the central nervous system and somite tissues of zebrafish embryos. Most of these effects are due to activation of p53-mediated apoptosis and can be suppressed by co-injection of an anti-p53 Morpholino along with the experimental Morpholino. Moreover, the p53-mediated apoptotic effect of a Morpholino knockdown has been phenocopied using another antisense structural type, showing the p53-mediated apoptosis to be a consequence of the loss of the targeted protein and not a consequence of the knockdown oligo type. It appears that these effects are sequence-specific; as in most cases, if a Morpholino is associated with non-target effects, the 4-base mismatch Morpholino will not trigger these effects.
A cause for concern in the use of Morpholinos is the potential for "off-target" effects. Whether an observed morphant phenotype is due to the intended knockdown or an interaction with an off-target RNA can often be addressed by running another experiment to confirm that the observed morphant phenotype results from the knockdown of the expected target. This can be done by recapitulating the morphant phenotype with a second, non-overlapping Morpholino targeting the same mRNA or by confirmation of the observed phenotypes by use of a mutant strain or dominant-negative methods. As mentioned above, rescue of observed phenotypes by coinjecting a rescue mRNA is, when feasible, a reliable test of specificity of a Morpholino.
For a Morpholino to be effective, it must be delivered past the cell membrane into the cytosol of a cell. Once in the cytosol, Morpholinos freely diffuse between the cytosol and nucleus, as demonstrated by the nuclear splice-modifying activity of Morpholinos observed after microinjection into the cytosol of cells. Different methods are used for delivery into embryos, into cultured cells or into adult animals. A microinjection apparatus is usually used for delivery into an embryo, with injections most commonly performed at the single-cell or few-cell stage; an alternative method for embryonic delivery is electroporation, which can deliver oligos into tissues of later embryonic stages. Common techniques for delivery into cultured cells include the Endo-Porter peptide (which causes the Morpholino to be released from endosomes), the Special Delivery system (no longer commercially available, used a Morpholino-DNA heteroduplex and an ethoxylated polyethylenimine delivery reagent), electroporation, or scrape loading.
Delivery into adult tissues is usually difficult, though there are a few systems allowing useful uptake of unmodified Morpholino oligos (including uptake into muscle cells with Duchenne muscular dystrophy or the vascular endothelial cells stressed during balloon angioplasty). Though they permeate through intercellular spaces in tissues effectively, unconjugated PMOs have limited distribution into the cytosol and nuclear spaces within healthy tissues following IV administration. Systemic delivery into many cells in adult organisms can be accomplished by using covalent conjugates of Morpholino oligos with cell-penetrating peptides, and, while toxicity has been associated with moderate doses of the peptide conjugates, they have been used in vivo for effective oligo delivery at doses below those causing observed toxicity. An octa-guanidinium dendrimer attached to the end of a Morpholino can deliver the modified oligo (called a Vivo-Morpholino) from the blood to the cytosol. Delivery-enabled Morpholinos, such as peptide conjugates and Vivo-Morpholinos, show promise as therapeutics for viral and genetic diseases.
- Summerton, J (1999). "Morpholino Antisense Oligomers: The Case for an RNase-H Independent Structural Type.". Biochimica et Biophysica Acta. 1489 (1): 141–58. PMID 10807004. doi:10.1016/S0167-4781(99)00150-5.
- Heasman J (2002). "Morpholino oligos: making sense of antisense?". Dev. Biol. 243 (2): 209–14. PMID 11884031. doi:10.1006/dbio.2001.0565.
- Draper, BW; Morcos, PA; Kimmel, CB (2001). "Inhibition of zebrafish fgf8 pre-mRNA splicing with Morpholino oligos: A quantifiable method for gene knockdown.". Genesis. 30 (3): 154–6. PMID 11477696. doi:10.1002/gene.1053.
- Kloosterman, WP; Lagendijk, AK; Ketting, RF; Moulton, JD; Plasterk, RHA (2007). "Targeted inhibition of miRNA maturation with morpholinos reveals a role for miR-375 in pancreatic islet development". PLoS Biol. 5 (8): e203. PMID 17676975. doi:10.1371/journal.pbio.0050203.
- Moulton, JD (2016). "Guide for Morpholino Users: Toward Therapeutics" (PDF). J Drug Discov Develop and Deliv. 3 (2): 1023.
- = Moulton, HM; Moulton, JD, eds. (2017). Morpholino Oligomers. Methods in Molecular Biology. 1565. Humana Press (Springer). p. 284. ISBN 978-1-4939-6815-2. doi:10.1007/978-1-4939-6817-6.
- Geller BL (2005). "Antibacterial antisense". Curr. Opin. Mol. Ther. 7 (2): 109–13. PMID 15844617.
- Deas, TS; Bennett CJ; Jones SA; Tilgner M; Ren P; Behr MJ; Stein DA; Iversen PL; Kramer LD; Bernard KA; Shi PY (2007). "In Vitro Resistance Selection and In Vivo Efficacy of Morpholino Oligomers against West Nile Virus". Antimicrob Agents Chemother. 51 (7): 2470–82. PMC . PMID 17485503. doi:10.1128/AAC.00069-07.
- McClorey, G; Fall AM; Moulton HM; Iversen PL; Rasko JE; Ryan M; Fletcher S; Wilton SD (2006). "Induced dystrophin exon skipping in human muscle explants". Neuromuscul Disord. 16 (9–10): 583–90. PMID 16919955. doi:10.1016/j.nmd.2006.05.017.
- "Press Announcements - FDA grants accelerated approval to first drug for Duchenne muscular dystrophy".
- Summerton, JE (2017), "Ch.1 Invention and Early History of Morpholinos: From Pipe Dream to Practical Products", Morpholino Oligomers, Methods in Molecular Biology, 1565, Humana Press (Springer), pp. 1–15, ISBN 978-1-4939-6817-6, doi:10.1007/978-1-4939-6817-6
- Summerton, J; Weller D (1997). "Morpholino Antisense Oligomers: Design, Preparation and Properties". Antisense & Nucleic Acid Drug Development. 7 (3): 187–95. PMID 9212909. doi:10.1089/oli.1.1997.7.187.
- Nasevicius, A; Ekker SC (2000). "Effective targeted gene 'knockdown' in zebrafish". Nature Genetics. 26 (2): 216–20. PMID 11017081. doi:10.1038/79951.
- Heasman, J; Kofron M; Wylie C (2000). "Beta-catenin signaling activity dissected in the early Xenopus embryo: a novel antisense approach". Developmental Biology. 222 (1): 124–34. PMID 10885751. doi:10.1006/dbio.2000.9720.
- Howard, EW; Newman LA; Oleksyn DW; Angerer RC; Angerer LM (2001). "SpKrl: a direct target of (beta)-catenin regulation required for endoderm differentiation in sea urchin embryos". Development. 128 (3): 365–75. PMID 11152635.
- Kos, R; Reedy MV; Johnson RL; Erickson CA (2001). "The winged-helix transcription factor FoxD3 is important for establishing the neural crest lineage and repressing melanogenesis in avian embryos". Development. 128 (8): 1467–79. PMID 11262245.
- Morcos, PA (2001). "Achieving efficient delivery of Morpholino oligos in cultured cells". Genesis. 30 (3): 94–102. PMID 11477682. doi:10.1002/gene.1039.
- Summerton, JE (2005). "Endo-porter: a novel reagent for safe, effective delivery of substances into cells". Ann N Y Acad Sci. 1058: 62–75. PMID 16394126. doi:10.1196/annals.1359.012.
- Morcos, PA; Li YF; Jiang S (2008). "Vivo-Morpholinos: A non-peptide transporter delivers Morpholinos into a wide array of mouse tissues". BioTechniques. 45 (6): 616–26. PMID 19238792. doi:10.2144/000113005.
- Stancheva, I; Collins AL; Van den Veyver IB; Zoghbi H; Meehan RR (2003). "A mutant form of MeCP2 protein associated with human Rett syndrome cannot be displaced from methylated DNA by notch in Xenopus embryos". Mol Cell. 12 (2): 425–35. PMID 14536082. doi:10.1016/S1097-2765(03)00276-4. See Figure 1
- "New molecule knocks out superbugs' immunity to antibiotics". newatlas.com. Retrieved 2017-01-25.
- K., Sully, Erin; L., Geller, Bruce; Lixin, Li,; M., Moody, Christina; M., Bailey, Stacey; L., Moore, Amy; Michael, Wong,; Patrice, Nordmann,; M., Daly, Seth. "Peptide-conjugated phosphorodiamidate morpholino oligomer (PPMO) restores carbapenem susceptibility to NDM-1-positive pathogens in vitro and in vivo". Journal of Antimicrobial Chemotherapy. doi:10.1093/jac/dkw476/2691388/peptide-conjugated-phosphorodiamidate-morpholino.
- Bruno, IG; Jin W; Cote GJ (2004). "Correction of aberrant FGFR1 alternative RNA splicing through targeting of intronic regulatory elements". Hum Mol Genet. 13 (20): 2409–20. PMID 15333583. doi:10.1093/hmg/ddh272.
- Vetrini, F; Tammaro R; Bondanza S; Surace EM; Auricchio A; De Luca M; Ballabio A; Marigo V (2006). "Aberrant splicing in the ocular albinism type 1 gene (OA1/GPR143) is corrected in vitro by Morpholino antisense oligonucleotides". Hum Mutat. 27 (5): 420–6. PMID 16550551. doi:10.1002/humu.20303.
- Morcos, PA (2007). "Achieving targeted and quantifiable alteration of mRNA splicing with Morpholino oligos". Biochem Biophys Res Commun. 358 (2): 521–7. PMID 17493584. doi:10.1016/j.bbrc.2007.04.172.
- König, H; Matter N; Bader R; Thiele W; Müller F (2007). "Splicing Segregation: The Minor Spliceosome Acts outside the Nucleus and Controls Cell Proliferation". Cell. 131 (4): 718–29. PMID 18022366. doi:10.1016/j.cell.2007.09.043.
- Kloosterman, WP; Wienholds E; Ketting RF; Plasterk RH (2004). "Substrate requirements for let-7 function in the developing zebrafish embryo". Nucleic Acids Res. 32 (21): 6284–91. PMC . PMID 15585662. doi:10.1093/nar/gkh968.
- Flynt, AS; Li N; Thatcher EJ; Solnica-Krezel L; Patton JG (2007). "Zebrafish miR-214 modulates Hedgehog signaling to specify muscle cell fate". Nature Genetics. 39 (2): 259–263. PMID 17220889. doi:10.1038/ng1953.
- Kloosterman, WP; Lagendijk AK; Ketting RF; Moulton JD; Plasterk RHA (2007). "Targeted Inhibition of miRNA Maturation with Morpholinos Reveals a Role for miR-375 in Pancreatic Islet Development". PLoS Biol. 5 (8): e203. PMC . PMID 17676975. doi:10.1371/journal.pbio.0050203.
- Lagendijk, AK; Moulton JD; Bakkers J (2012). "Revealing details: whole mount microRNA in situ hybridization protocol for zebrafish embryos and adult tissues". Bio Open. doi:10.1242/bio.2012810.
- Yen, L; Svendsen J; Lee JS; Gray JT; Magnier M; Baba T; D'Amato RJ; Mulligan RC (2004). "Exogenous control of mammalian gene expression through modulation of RNA self-cleavage". Nature. 431 (7007): 471–6. PMID 15386015. doi:10.1038/nature02844.
- Matter, N; Konig H (2005). "Targeted 'knockdown' of spliceosome function in mammalian cells". Nucleic Acids Res. 33 (4): e41. PMC . PMID 15731334. doi:10.1093/nar/gni041.
- Howard MT, Gesteland RF, Atkins JF (2004). "Efficient stimulation of site-specific ribosome frameshifting by antisense oligonucleotides". RNA. 10 (10): 1653–61. PMC . PMID 15383681. doi:10.1261/rna.7810204.
- Penn, AC; Balik A; Greger IH (2012). "Steric antisense inhibition of AMPA receptor Q/R editing reveals tight coupling to intronic editing sites and splicing". Nucleic Acids Res. 41 (2): 1113–23. PMC . PMID 23172291. doi:10.1093/nar/gks1044.
- Bill, BR; Petzold AM; Clark KJ; Schimmenti LA; Ekker SC (2009). "A Primer for Morpholino Use in Zebrafish". Zebrafish. 6 (1): 69–77. PMC . PMID 19374550. doi:10.1089/zeb.2008.0555.
- Kamachi, Y; Okuda Y; Kondoh H (2008). "Quantitative Assessment of the Knockdown Efficiency of Morpholino Antisense Oligonucleotides in Zebrafish Embryos Using a Luciferase Assay". Genesis. 46 (1): 1–7. PMID 18196596. doi:10.1002/dvg.20361.
- Hudziak, RM; Barofsky E; Barofsky DF; Weller DL; Huang SB; Weller DD (1996). "Resistance of Morpholino phosphorodiamidate oligomers to enzymatic degradation". Antisense Nucleic Acid Drug Dev. 6 (4): 267–72. PMID 9012862. doi:10.1089/oli.1.1996.6.267.
- Youngblood, DS; Hatlevig SA; Hassinger JN; Iversen PL; Moulton HM (2007). "Stability of cell-penetrating peptide-Morpholino oligomer conjugates in human serum and in cells". Bioconjug Chem. 18 (1): 50–60. PMID 17226957. doi:10.1021/bc060138s.
- Ekker, SC; Larson JD (2001). "Morphant Technology in Model Developmental Systems". Genesis. 30 (3): 89–93. PMID 11477681. doi:10.1002/gene.1038.
- Robu, ME; Larson JD; Nasevicius A; Beiraghi S; Brenner C; Farber SA; Ekker SC (2007). "p53 Activation by Knockdown Technologies". PLoS Genetics. 3 (5): e78. PMC . PMID 17530925. doi:10.1371/journal.pgen.0030078.
- Rosen, JN; Sweeny MF; Mably JD (2009). "Microinjection of Zebrafish Embryos to Analyze Gene Function". J Vis Exp. 9 (25). PMC . PMID 19274045. doi:10.3791/1115.
- Cerda, GA; Thomas JE; Allende ML; Karlstrom RO; Palma V (2006). "Electroporation of DNA, RNA, and Morpholinos into zebrafish embryos". Methods. 39 (3): 207–11. PMID 16837210. doi:10.1016/j.ymeth.2005.12.009.
- Jubin, R (2004). "Optimizing electroporation conditions for intracellular delivery of Morpholino antisense oligonucleotides directed against the hepatitis C virus internal ribosome entry site". Methods Mol Med. 106: 309–22. PMID 15375324. doi:10.1385/1-59259-854-4:309.
- Partridge, M; Vincent A; Matthews P; Puma J; Stein D; Summerton J (1996). "A simple method for delivering Morpholino antisense oligos into the cytoplasm of cells". Antisense Nucleic Acid Drug Dev. 6 (3): 169–75. PMID 8915501. doi:10.1089/oli.1.1996.6.169.
- Fletcher, S; Honeyman K; Fall AM; Harding PL; Johnsen RD; Wilton SD (2006). "Dystrophin expression in the mdx mouse after localised and systemic administration of a Morpholino antisense oligonucleotide". J Gene Med. 8 (2): 207–16. PMID 16285002. doi:10.1002/jgm.838.
- Kipshidze NN, Kim HS, Iversen P, Yazdi HA, Bhargava B, New G, Mehran R, Tio F, Haudenschild C, Dangas G, Stone GW, Iyer S, Roubin GS, Leon MB, Moses JW (2002). "Intramural coronary delivery of advanced antisense oligonucleotides reduces neointimal formation in the porcine stent restenosis model". J Am Coll Cardiol. 39 (10): 1686–91. PMID 12020498. doi:10.1016/S0735-1097(02)01830-2.
- Abes, S; Moulton HM; Clair P; Prevot P; Youngblood DS; Wu RP; Iversen PL; Lebleu B (2006). "Vectorization of Morpholino oligomers by the (R-Ahx-R)(4) peptide allows efficient splicing correction in the absence of endosomolytic agents". J Control Release. 116 (3): 304–13. PMID 17097177. doi:10.1016/j.jconrel.2006.09.011.
- Burrer, R; Neuman BW; Ting JPC; Stein DA; Moulton HM; Iversen PL; Kuhn P; Buchmeier MJ (2007). "Antiviral Effects of Antisense Morpholino Oligomers in Murine Coronavirus Infection Models". J. Virol. 81 (11): 5637–48. PMC . PMID 17344287. doi:10.1128/JVI.02360-06.
- Amantana, A; Moulton HM; Cate ML; Reddy MT; Whitehead T; Hassinger JN; Youngblood DS; Iversen PL (2007). "Pharmacokinetics, Biodistribution, Stability and Toxicity of a Cell-Penetrating Peptide-Morpholino Oligomer Conjugate". Bioconjug Chem. 18 (4): 1325–31. PMID 17583927. doi:10.1021/bc070060v.
- Li, YF; Morcos PA (2008). "Design and Synthesis of Dendritic Molecular Transporter that Achieves Efficient in Vivo Delivery of Morpholino Antisense Oligo". Bioconjug Chem. 19 (7): 1464–70. PMID 18564870. doi:10.1021/bc8001437.
- Moulton, JD; Jiang S (2009). "Gene Knockdowns in Adult Animals: PPMOs and Vivo-Morpholinos". Molecules. 14 (3): 1304–23. PMID 19325525. doi:10.3390/molecules14031304.
- Wiley-Liss, Inc. Special Issue: Morpholino Gene Knockdowns of genesis Volume 30, Issue 3 Pages 89-200 (July 2001). This is a special issue of Genesis that consists of a series of peer-reviewed short papers using Morpholino knock downs of gene function in various animal and tissue culture systems.
- "Peptide Nucleic Acids, Morpholinos and Related Antisense Biomolecules." eds. Janson & During (Springer, 2007)
- Moulton, Jon (2007). "Using Morpholinos to Control Gene Expression (Unit 4.30)". In Beaucage, Serge. Current Protocols in Nucleic Acid Chemistry. New Jersey: John Wiley & Sons, Inc. ISBN 978-0-471-24662-6.