Mota'ain, also spelled Mota'in or Mota Ain, is a village in Bobonaro District in East Timor and is the country's main road border crossing into West Timor in Indonesia. The East Timor-Indonesia border runs along the River Mota.
Mota'ain is 118 km west of the East Timor capital Dili and 3 km west of the town of Batugade.
The first town across the border in Indonesia is Atapupu, 25 km from Atambua, the seat of the Belu Regency of the Nusa Tenggara Timur Province in West Timor. The Indonesian customs, immigration and quarantine post at Mota'ain is called the Atambua checkpoint.