Movat's stain is a pentachrome stain originally developed by Henry Zoltan Movat (1923–1995), a Romanian-Canadian Pathologist in Toronto in 1955 to highlight the various constituents of connective tissue, especially cardiovascular tissue, by five colors in a single stained slide. In 1972, H. K. Russell, Jr. modified the technique so as to reduce the time for staining and to increase the consistency and reliability of the staining.
|Black||Nuclei; elastic fibres|
|Yellow||Collagen fibres; reticular fibres|
|Blue||Ground substance; mucin|
The modified Russel-Movat staining highlights a lot of tissue components in histological slides. It is obtained by a mix of five stains: alcian blue, Verhoeff hematoxylin, crocein scarlet combined with acidic fuchsine and saffron. At pH 2.5, alcian blue is fixed by electrostatic binding with the acidic mucopolysaccharides. The Verhoeff hematoxylin has a high affinity for nuclei and elastin fibers, negatively charged. The combination of crocein scarlet with acidic fuchsine stains acidophilic tissue components in red. Then, collagen and reticulin fibers are unstained by a reaction with phosphotungstic acid and stained in yellow by saffron.
Movat's stain showing amyloid (brown) and fibrosis (yellow) of the heart.
- "Pathology News: Newsletter, Vol. 3, No. 4: April 1996" (PDF). Department of Pathology and Molecular Medicine, Queen's University.
- Movat, HZ (1955). "Demonstration of all connective tissue elements in a single section; pentachrome stains". AMA Archives of Pathology. 60 (3): 289–95. PMID 13248341.
- Russell Jr, HK (1972). "A modification of Movat's pentachrome stain". Archives of Pathology. 94 (2): 187–91. PMID 4114784.
- "Penn MCRC > Modified Movat's Pentachrome Stain". Perelman School of Medicine, the University of Pennsylvania. Retrieved 2012-08-08.
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