Murid herpesvirus 68

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Murid herpesvirus 68
Virus classification
Group: Group I (dsDNA)
Order: Herpesvirales
Family: Herpesviridae
Subfamily: Gammaherpesvirinae
Genus: Rhadinovirus
Species: Murid herpesvirus 4
Subspecies: Murid herpesvirus 68 (MHV-68)

Murid herpesvirus 68 (MHV-68) aka murine herpesvirus 68 is an isolate of Murid herpesvirus 4 which is a virus in the genus Rhadinovirus. It is a member of the subfamily Gammaherpesvirinae in the family of Herpesviridae. MHV-68 serves as a model for study of human gammaherpesviruses which cause significant human disease including B-cell lymphoma and Kaposi's sarcoma. The WUMS strain of MHV-68 was fully sequenced and annotated in 1997,[1] and the necessity of most of its genes in viral replication was characterized by random transposon mutagenesis study .[2]

MHV-68 surface proteins[edit]

Alpha-, beta-, and gammaherpesviruses display a heterodimer composed of glycoprotein H (gH) and glycoprotein L (gL) on their envelopes. This receptor is involved in the cell-to-cell transmission of the virus. Glycoprotein H has two conformations. Glycoprotein L is a chaperone protein which assures that gH takes on the correct conformation. When herpesviruses lack gL, gH misfolds. When alpha- or betaherpesviruses lack gL, they are noninfectious. When Murine Gammaherpesvirus 68 lacks gL, it remains infectious but is less able to bind to fibroblasts and epithelial cells.[3]

The open reading frame M7 of the MHV-68 genome encodes for the surface receptor glycoprotein 150 (gp150). It is homologous to the Epstein-Barr virus membrane antigen gp350/220.[4] MHV-68 is more closely related to the Kaposi's Sarcoma-associated herpesvirus (KSHV) than it is to the Epstein-Barr virus. Glycoprotein K8.1 is the KSHV homolog of MHV-68 gp150.[5] MHV-68 is a very close relative of MHV-72. The MHV-68 M7 gene differs from the corresponding MHV-72 gene by five point mutations which alter four codons.[4] Glycoprotein 150 allows MHV-68 to bind to B-cells.[5]

References[edit]

  1. ^ Herbet W. Virgin IV; Philip Latreille; Pamela Wamsley; Kymberlie Hallsworth; Karen E. Weck; Albert J. Dal Canto & Samuel H. Speck (August 1997). "Complete sequence and genomic analysis of murine gammaherpesvirus 68". Journal of Virology. 71 (8): 5894–904. PMC 191845Freely accessible. PMID 9223479. 
  2. ^ Moon Jung Song; Seungmin Hwang; Wendy H. Wong; Ting-Ting Wu; Sangmi Lee; Hsiang-I Liao & Ren Sun (March 2005). "Identification of viral genes essential for replication of murine γ-herpesvirus 68 using signature-tagged mutagenesis". Proceedings of the National Academy of Sciences of the United States of America. 102 (10): 3805–10. doi:10.1073/pnas.0404521102. PMC 553290Freely accessible. PMID 15738413. 
  3. ^ Laurent Gillet; Janet S. May; Susanna Colaco & Philip G. Stevenson (January 2007). "Glycoprotein L Disruption Reveals Two Functional Forms of the Murine Gammaherpesvirus 68 Glycoprotein H". Journal of Virology. 81 (1): 280–291. doi:10.1128/jvi.01616-06. PMC 1797276Freely accessible. PMID 17050601. 
  4. ^ a b Macáková K, Matis J, Rezuchová I, Kúdela O, Raslová H, Kúdelová M (Jan 2003). "Murine gammaherpesvirus (MHV) M7 gene encoding glycoprotein 150 (gp150): difference in the sequence between 72 and 68 strains". Virus Genes. 26 (1): 89–95. PMID 12683351. 
  5. ^ a b James P. Stewart; Ondine J. Silvia; Isobel M. D. Atkin; David J. Hughes; Bahram Ebrahimi & Heiko Adler (October 2004). "In Vivo Function of a Gammaherpesvirus Virion Glycoprotein: Influence on B-Cell Infection and Mononucleosis". Journal of Virology. 78 (19): 10449–10459. doi:10.1128/jvi.78.19.10449-10459.2004. PMC 516434Freely accessible. PMID 15367611.