Torkko et al. 2000, ATCC 700701
Mycobacterium botniense is a slowly growing Mycobacterium, which produces a yellow pigment. It was first isolated from a stream of water. M. botniense is most closely related to Mycobacterium xenopi. Etymology: botniense; of Botnia, referring to the Latin name of the province of Finland from which the isolation was made.
- Gram-positive, nonmotile and acid-fast rods.
- Colonies on Löwenstein-Jensen media and on Middlebrook 7H11 agar are small, dysgonic and scotochromogenic, and produce yellow pigment.
- Visible growth from diluted inocula requires 5 to 8 weeks. Growth occurs at 37 to 50 °C.
- The type strain is positive for 10-d arylsulfatase and pyrazinamidase.
- Negative for 3-d arylsulfatase, urease, nitrate reductase, semi-quantitative catalase, heat-stable catalase, acid phosphatase, b-galactosidase and 5% NaCl tolerance.
- Tween 80 is not hydrolysed in 10 d.
- A phylogenetic tree based on the evaluation of 16S rDNA sequences places M. botniense among the slow-growing mycobacteria, closest to M. xenopi.
- Not known, but first isolated from an environmental source.
- First isolated in Finland from stream waters. Strain E347 = ATCC 700701 = CCUG 47976 = CIP 106753 = DSM 44537.
- Torkko P. 2000., Mycobacterium xenopi and related organisms isolated from stream waters in Finland and description of Mycobacterium botniense sp. nov. Int. J. Syst. Evol. Microbiol., 50, 283-289.
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