|Microphotograph of Mycobacterium leprae, the small brick-red rods in clusters, taken from a skin lesion. Source: CDC|
Mycobacterium leprae, also known as Hansen’s bacillus spirilly, mostly found in warm tropical countries, is a bacterium that causes leprosy (Hansen's disease). It is an intracellular, pleomorphic, acid-fast, pathogenic bacterium. M. leprae is an aerobic bacillus (rod-shaped) surrounded by the characteristic waxy coating unique to mycobacteria. In size and shape, it closely resembles Mycobacterium tuberculosis. Due to its thick waxy coating, M. leprae stains with a carbol fuchsin rather than with the traditional Gram stain. The culture takes several weeks to mature.
It was discovered in 1873 by the Norwegian physician Gerhard Armauer Hansen, who was searching for the bacteria in the skin nodules of patients with leprosy. It was the first bacterium to be identified as causing disease in humans.[verification needed] The organism has been successfully grown on an artificial cell culture medium on a very limited basis by researcher Arvind Dhople. Since in vitro cultivation is not generally possible, it has instead been grown in mouse foot pads and more recently in nine-banded armadillos because they, like humans, are susceptible to leprosy. Armadillos have a much lower body temperature than most mammals, and the bacteria are often found in their lungs, liver, and spleen. This can be used as a diagnostic test for the presence of bacilli in body lesions of suspected leprosy patients. The difficulty in culturing the organism appears to be because it is an obligate intracellular parasite that lacks many necessary genes for independent survival. The complex and unique cell wall that makes members of the genus Mycobacterium difficult to destroy is apparently also the reason for the extremely slow replication rate.
Mycobacterium leprae was sensitive to dapsone (diaminodiphenylsulfone, the first effective treatment which was discovered for leprosy in the 1940s), but resistance against this antibiotic has developed over time. Therapy with dapsone alone is now strongly contraindicated. Currently, a multidrug treatment (MDT) is recommended by the World Health Organization, including dapsone, rifampicin. and clofazimine. In patients receiving the MDT, a high proportion of the bacilli die within a short amount of time without immediate relief of symptoms. This suggests many symptoms of leprosy must be due in part to the presence of dead cells.
The incubation period of M. leprae can range between 9 months and 20 years. It replicates intracellularly inside histiocytes and nerve cells and has two forms. One form is "tuberculoid", which induces a cell-mediated response that limits its growth. Through this form, M. leprae multiplies at the site of entry, usually the skin, invading and colonizing Schwann cells. The microbe then induces T-helper lymphocytes, epitheloid cells, and giant cell infiltration of the skin, causing infected individuals to exhibit large flattened patches with raised and elevated red edges on their skin. These patches have dry, pale, hairless centers, accompanied by a loss of sensation on the skin. The loss of sensation may develop as a result of invasion of the peripheral sensory nerves. The macule at the cutaneous site of entry and the loss of pain sensation are key clinical indications that an individual has a tuberculoid form of leprosy.
The second form of leprosy is the "lepromatous" form, in which the microbes proliferate within the macrophages at the site of entry. They also grow within the epithelial tissues of the face and ear lobes. The suppressor T-cells that are induced are numerous, but the epithelioid and giant cells are rare or absent. With cell-mediated immunity impaired, large numbers of M. leprae appear in the macrophages and the infected patients develop papules at the entry site, marked by a folding of the skin. Gradual destruction of cutaneous nerves lead to what is referred to as "classic lion face". Extensive penetration of this microbe may lead to severe body damage; for example the loss of bones, fingers, and toes.
M. leprae has the longest doubling time of all known bacteria and has thwarted every effort at culture in the laboratory. Comparing the genome sequence of M. leprae with that of M. tuberculosis provides clear explanations for these properties, and reveals an extreme case of reductive evolution. Less than half of the genome contains functional genes. Gene deletion and decay appear to have eliminated many important metabolic activities, including siderophore production, part of the oxidative and most of the microaerophilic and anaerobic respiratory chains, and numerous catabolic systems and their regulatory circuits.
The first genome sequence of a strain of M. leprae was completed in 1998. The genome sequence of a strain originally isolated in Tamil Nadu, India, and designated TN, was completed in 2013. The sequence was obtained by a combined approach, employing automated DNA sequence analysis of selected cosmids and whole-genome 'shotgun' clones. After the finishing process, the genome sequence was found to contain 3,268,203 base pairs (bp), and to have an average G+C content of 57.8%, values much lower than the corresponding values for M. tuberculosis, which are 4,441,529 bp and 65.6% G+C.
Evolution and pseudogenes
M. leprae has undergone a dramatic reduction in genome size with the loss of many genes. This genome reduction is not complete and numerous genes are still present as nonfunctional pseudogenes. Downsizing from a genome of 4.42 Mbp, such as that of M. tuberculosis, to one of 3.27 Mbp would account for the loss of some 1200 protein-coding sequences. Some evidence shows that many of the genes that were present in the genome of the common ancestor of M. leprae and M. tuberculosis have been lost in the M. leprae genome. 1500 genes are still common to both M. leprae and M. tuberculosis.
Information from the completed genome can be useful to develop diagnostic skin tests, to understand the mechanisms of nerve damage and drug resistance, and to identify novel drug targets for rational design of new therapeutic regimens and drugs to treat leprosy and its complications.
Ancient M. leprae
Almost complete sequences of M. leprae from medieval skeletons with osteological lesions suggestive of leprosy from different Europe geographic origins were obtained using DNA capture techniques and high-throughput sequencing. Ancient sequences were compared with those of modern strains from biopsies of leprosy patients representing diverse genotypes and geographic origins, giving new insights in the understanding of its evolution and course through history, phylogeography of the leprosy bacillus, and the disappearance of leprosy from Europe.
Verena J. Schuenemann et al. demonstrated a remarkable genomic conservation during the past 1000 years and a close similarity between modern and ancient strains, suggesting that the sudden decline of leprosy in Europe was not due to a loss of virulence, but due to extraneous factors, such as other infectious diseases, changes in host immunity, or improved social conditions.
The geographic occurrences of M. leprae include: Angola, Brazil, Central African Republic, the Democratic Republic of Congo, Federated States of Micronesia, India, Kiribati, Madagascar, Nepal, Republic of Marshall Islands, and the United Republic of Tanzania.
The closest relative to M. leprae is M. lepromatosis. These species diverged (95% highest posterior density – ) The most recent common ancestor of the extant M. leprae strains was calculated to have lived 3,607 years ago [95% highest posterior density 2204–5525 years ago]. The estimated substitution rate was 7.67 x 10−9 substitutions per site per year, similar to other bacteria.
A study of genomes isolated from medieval cases estimated the mutation rate to be 6.13 × 10−9. The authors also showed that the leprosy bacillus in the Americas was brought there from Europe.
The ancestors of M. leprae and M. tuberculosis have been estimated to have separated .
Another study suggests that M. leprae originated in East Africa and spread from there to Europe and the Middle East initially before spreading to West Africa and the Americas in the last 500 years.
Diagnostic criteria for leprosy
The diagnosis of leprosy is primarily a clinical one. In one Ethiopian study, the following criteria had a sensitivity of 94% with a positive predictive value of 98% in diagnosing leprosy. Diagnosis was based on one or more of three signs:
- Hypopigmented or reddish skin patches with definite loss of sensation
- Thickened peripheral nerves
- Acid-fast bacilli on skin smears or biopsy material
Multidrug therapy (MDT) uses antibiotics that destroy M. leprae bacilli, including: dapsone, rifampin, rifabutin, clofazamine, fluoroquinolones, macrolides, and minocycline.
A preventive measure of M. leprae is to avoid close contact with infectious people who are untreated. The Bacillus Calmette–Guérin vaccine offers a variable amount of protection against leprosy in addition to its main target of tuberculosis. Armadillos are also known hosts for Mycobacterium leprae and have infected humans in the southeastern United States, although the geographic range of the disease and its complexity has been spreading.
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