|3D model (Jmol)||Interactive image|
|Molar mass||281.27 g·mol−1|
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
N6-Methyladenosine (m6A) is an abundant modification in mRNA and is found within some viruses, and most eukaryotes including mammals, insects, plants and yeast. It is also found in tRNA, rRNA, and small nuclear RNA (snRNA) as well as several long non-coding RNA, such as Xist.
Adenosine methylation is directed by a large m6A methyltransferase complex containing METTL3 as the SAM-binding sub-unit. In vitro, this methyltransferase complex preferentially methylates RNA oligonucleotides containing GGACU and a similar preference was identified in vivo in mapped m6A sites in Rous sarcoma virus genomic RNA and in bovine prolactin mRNA. More recent studies have characterized other key components of the m6A methyltransferase complex in mammals, including METTL14, Wilms tumor 1 associated protein (WTAP) and KIAA1429. Following a 2010 speculation of m6A in mRNA being dynamic and reversible, the discovery of the first m6A demethylase, fat mass and obesity-associated protein (FTO) in 2011 confirmed this hypothesis and revitalized the interests in the study of m6A. A second m6A demethylase alkB homolog 5 (ALKBH5) was later discovered as well.
The biological functions of m6A are mediated through a group of RNA binding proteins that specifically recognize the methylated adenosine on RNA. These binding proteins are named m6A readers. The YT521-B homology (YTH) domain family of proteins (YTHDF1, YTHDF2, YTHDF3 and YTHDC1) have been characterized as direct m6A readers and have a conserved m6A-binding pocket. These m6A readers, together with m6A methyltransferases (writers) and demethylases (erasers), establish a complex mechanism of m6A regulation in which writers and erasers determine the distributions of m6A on RNA, whereas readers mediate m6A-dependent functions. m6A has also been shown to mediate a structural switch termed m6A switch.
In budding yeast (Sacharomyces cerevisiae), the homologue of METTL3, IME4 is induced in diploid cells in response to nitrogen and fermentable carbon source starvation and is required for mRNA methylation and the initiation of correct meiosis and sporulation. mRNAs of IME1 and IME2, key early regulators of meiosis, are known to be targets for methylation, as are transcripts of IME4 itself.
Mutations of MTA, the Arabidopsis thaliana homologue of METTL3, results in embryo arrest at the globular stage. A >90% reduction of m6A levels in mature plants leads to dramatically altered growth patterns and floral homeotic abnormalities.
Mapping of m6A in human and mouse RNA has identified over 18,000 m6A sites in the transcripts of more than 7,000 human genes with a consensus sequence of [G/A/U][G>A]m6AC[U>A/C] consistent with the previously identified motif. The localization of individual m6A sites in many mRNAs is highly similar between human and mouse, and transcriptome-wide analysis reveals that m6A is found in regions of high evolutionary conservation. m6A is found within long internal exons and is preferentially enriched within 3’ UTRs and around stop codons. m6A within 3’ UTRs is also associated with the presence of microRNA binding sites; roughly 2/3 of the mRNAs which contain an m6A site within their 3’ UTR also have at least one microRNA binding site. By integrating all m6A sequencing data, a novel database called RMBase has identified and provided ~200,000 N6-Methyladenosines (m6A) sites in the human and mouse genomes.
Precise m6A mapping by m6A-CLIP/IP  (briefly m6A-CLIP, in multiple tissues/cultured cells of mouse and human) revealed that a majority of m6A locates in the last exon of mRNAs, and the m6A enrichment around stop codons is a coincidence that many stop codons locate round the start of last exons where m6A is truly enriched. The major presence of m6A in last exon (>=70%) allows the potential for 3'UTR regulation, including alternative polyadenylation.
m6A is susceptible to dynamic regulation both throughout development and in response to cellular stimuli. Analysis of m6A in mouse brain RNA reveals that m6A levels are low during embryonic development and increase dramatically by adulthood. Additionally, silencing the m6A methyltransferase significantly affects gene expression and alternative RNA splicing patterns, resulting in modulation of the p53 (also known as TP53) signalling pathway and apoptosis.
The importance of m6A methylation for physiological processes was recently demonstrated. Inhibition of m6A methylation via pharmacological inhibition of cellular methylations or more specifically by siRNA-mediated silencing of the m6A methylase Mettl3 led to the elongation of the circadian period. In contrast, overexpression of Mettl3 led to a shorter period. The mammalian circadian clock, composed of a transcription feedback loop tightly regulated to oscillate with a period of about 24 hours, is therefore extremely sensitive to perturbations in m6A-dependent RNA processing, likely due to the presence of m6A sites within clock gene transcripts.
Considering the versatile functions of m6A in various physiological processes, it is thus not surprising to find links between m6A and numerous human diseases; many originated from mutations or single nucleotide polymorphisms (SNPs) of cognate factors of m6A. The linkages between m6A and numerous cancer types have been indicated in reports that include stomach cancer, prostate cancer, breast cancer, pancreatic cancer, kidney cancer, mesothelioma, sarcoma, and leukaemia. The impacts of m6A on cancer cell proliferation might be much more profound with more data emerging. The depletion of METTL3 is known to cause apoptosis of cancer cells and reduce invasiveness of cancer cells, while the activation of ALKBH5 by hypoxia was shown to cause cancer stem cell enrichment. m6A has also been indicated in the regulation of energy homeostasis and obesity, as FTO is a key regulatory gene for energy metabolism and obesity. SNPs of FTO have been shown to associate with body mass index in human populations and occurrence of obesity and diabetes. The influence of FTO on pre-adipocyte differentiation has been suggested. The connection between m6A and neuronal disorders has also been studied. For instance, neurodegenerative diseases may be affected by m6A as the cognate dopamine signalling was shown to be dependent on FTO and correct m6A methylation on key signalling transcripts. The mutations in HNRNPA2B1, a potential reader of m6A, have been known to cause neurodegeneration.
Additionally, m6A has been reported to impact viral infections. Many RNA viruses including SV40, adenovirus, herpes virus, Rous sarcoma virus, and influenza virus have been known to contain the internal m6A methylation on virus genomic RNA. Several more recent studies have revealed that m6A regulators govern the efficiency of infection and replication of RNA viruses such as human immunodeficiency virus (HIV), hepatitis C virus (HCV), and Zika virus (ZIKV). These results suggest m6A and its cognate factors play crucial roles in regulating virus life cycle and host-viral interactions.
- Beemon K, Keith J (June 1977). "Localization of N6-methyladenosine in the Rous sarcoma virus genome". Journal of Molecular Biology. 113 (1): 165–79. doi:10.1016/0022-2836(77)90047-X. PMID 196091.
- Aloni Y, Dhar R, Khoury G (October 1979). "Methylation of nuclear simian virus 40 RNAs". Journal of Virology. 32 (1): 52–60. PMC . PMID 232187.
- Desrosiers R, Friderici K, Rottman F (October 1974). "Identification of methylated nucleosides in messenger RNA from Novikoff hepatoma cells". Proceedings of the National Academy of Sciences of the United States of America. 71 (10): 3971–5. doi:10.1073/pnas.71.10.3971. PMC . PMID 4372599.
- Adams JM, Cory S (May 1975). "Modified nucleosides and bizarre 5'-termini in mouse myeloma mRNA". Nature. 255 (5503): 28–33. doi:10.1038/255028a0. PMID 1128665.
- Wei CM, Gershowitz A, Moss B (January 1976). "5'-Terminal and internal methylated nucleotide sequences in HeLa cell mRNA". Biochemistry. 15 (2): 397–401. doi:10.1021/bi00647a024. PMID 174715.
- Perry RP, Kelley DE, Friderici K, Rottman F (April 1975). "The methylated constituents of L cell messenger RNA: evidence for an unusual cluster at the 5' terminus". Cell. 4 (4): 387–94. doi:10.1016/0092-8674(75)90159-2. PMID 1168101.
- Levis R, Penman S (April 1978). "5'-terminal structures of poly(A)+ cytoplasmic messenger RNA and of poly(A)+ and poly(A)- heterogeneous nuclear RNA of cells of the dipteran Drosophila melanogaster". Journal of Molecular Biology. 120 (4): 487–515. doi:10.1016/0022-2836(78)90350-9. PMID 418182.
- Nichols JL (1979). "In maize poly(A)-containing RNA". Plant Science Letters. 15 (4): 357–361. doi:10.1016/0304-4211(79)90141-X.
- Kennedy TD, Lane BG (June 1979). "Wheat embryo ribonucleates. XIII. Methyl-substituted nucleoside constituents and 5'-terminal dinucleotide sequences in bulk poly(AR)-rich RNA from imbibing wheat embryos". Canadian Journal of Biochemistry. 57 (6): 927–31. doi:10.1139/o79-112. PMID 476526.
- Zhong S, Li H, Bodi Z, Button J, Vespa L, Herzog M, Fray RG (May 2008). "MTA is an Arabidopsis messenger RNA adenosine methylase and interacts with a homolog of a sex-specific splicing factor". The Plant Cell. 20 (5): 1278–88. doi:10.1105/tpc.108.058883. PMC . PMID 18505803.
- Clancy MJ, Shambaugh ME, Timpte CS, Bokar JA (October 2002). "Induction of sporulation in Saccharomyces cerevisiae leads to the formation of N6-methyladenosine in mRNA: a potential mechanism for the activity of the IME4 gene". Nucleic Acids Research. 30 (20): 4509–18. doi:10.1093/nar/gkf573. PMC . PMID 12384598.
- Bodi Z, Button JD, Grierson D, Fray RG (September 2010). "Yeast targets for mRNA methylation". Nucleic Acids Research. 38 (16): 5327–35. doi:10.1093/nar/gkq266. PMC . PMID 20421205.
- Meyer KD, Saletore Y, Zumbo P, Elemento O, Mason CE, Jaffrey SR (June 2012). "Comprehensive analysis of mRNA methylation reveals enrichment in 3' UTRs and near stop codons". Cell. 149 (7): 1635–46. doi:10.1016/j.cell.2012.05.003. PMC . PMID 22608085.
- Dominissini D, Moshitch-Moshkovitz S, Schwartz S, Salmon-Divon M, Ungar L, Osenberg S, Cesarkas K, Jacob-Hirsch J, Amariglio N, Kupiec M, Sorek R, Rechavi G (April 2012). "Topology of the human and mouse m6A RNA methylomes revealed by m6A-seq". Nature. 485 (7397): 201–6. doi:10.1038/nature11112. PMID 22575960.
- Bokar JA, Shambaugh ME, Polayes D, Matera AG, Rottman FM (November 1997). "Purification and cDNA cloning of the AdoMet-binding subunit of the human mRNA (N6-adenosine)-methyltransferase". Rna. 3 (11): 1233–47. PMC . PMID 9409616.
- Harper JE, Miceli SM, Roberts RJ, Manley JL (October 1990). "Sequence specificity of the human mRNA N6-adenosine methylase in vitro". Nucleic Acids Research. 18 (19): 5735–41. doi:10.1093/nar/18.19.5735. PMC . PMID 2216767.
- Kane SE, Beemon K (September 1985). "Precise localization of m6A in Rous sarcoma virus RNA reveals clustering of methylation sites: implications for RNA processing". Molecular and Cellular Biology. 5 (9): 2298–306. doi:10.1128/mcb.5.9.2298. PMC . PMID 3016525.
- Horowitz S, Horowitz A, Nilsen TW, Munns TW, Rottman FM (September 1984). "Mapping of N6-methyladenosine residues in bovine prolactin mRNA". Proceedings of the National Academy of Sciences of the United States of America. 81 (18): 5667–71. doi:10.1073/pnas.81.18.5667. PMC . PMID 6592581.
- Liu J, Yue Y, Han D, Wang X, Fu Y, Zhang L, Jia G, Yu M, Lu Z, Deng X, Dai Q, Chen W, He C (February 2014). "A METTL3-METTL14 complex mediates mammalian nuclear RNA N6-adenosine methylation". Nature Chemical Biology. 10 (2): 93–5. doi:10.1038/nchembio.1432. PMC . PMID 24316715.
- Wang Y, Li Y, Toth JI, Petroski MD, Zhang Z, Zhao JC (February 2014). "N6-methyladenosine modification destabilizes developmental regulators in embryonic stem cells". Nature Cell Biology. 16 (2): 191–8. doi:10.1038/ncb2902. PMC . PMID 24394384.
- Ping XL, Sun BF, Wang L, Xiao W, Yang X, Wang WJ, Adhikari S, Shi Y, Lv Y, Chen YS, Zhao X, Li A, Yang Y, Dahal U, Lou XM, Liu X, Huang J, Yuan WP, Zhu XF, Cheng T, Zhao YL, Wang X, Rendtlew Danielsen JM, Liu F, Yang YG (February 2014). "Mammalian WTAP is a regulatory subunit of the RNA N6-methyladenosine methyltransferase". Cell Research. 24 (2): 177–89. doi:10.1038/cr.2014.3. PMC . PMID 24407421.
- Schwartz S, Mumbach MR, Jovanovic M, Wang T, Maciag K, Bushkin GG, Mertins P, Ter-Ovanesyan D, Habib N, Cacchiarelli D, Sanjana NE, Freinkman E, Pacold ME, Satija R, Mikkelsen TS, Hacohen N, Zhang F, Carr SA, Lander ES, Regev A (July 2014). "Perturbation of m6A writers reveals two distinct classes of mRNA methylation at internal and 5' sites". Cell Reports. 8 (1): 284–96. doi:10.1016/j.celrep.2014.05.048. PMC . PMID 24981863.
- He C (December 2010). "Grand challenge commentary: RNA epigenetics?". Nature Chemical Biology. 6 (12): 863–5. doi:10.1038/nchembio.482. PMID 21079590.
- Jia G, Fu Y, Zhao X, Dai Q, Zheng G, Yang Y, Yi C, Lindahl T, Pan T, Yang YG, He C (October 2011). "N6-methyladenosine in nuclear RNA is a major substrate of the obesity-associated FTO". Nature Chemical Biology. 7 (12): 885–7. doi:10.1038/nchembio.687. PMC . PMID 22002720.
- Zheng G, Dahl JA, Niu Y, Fedorcsak P, Huang CM, Li CJ, Vågbø CB, Shi Y, Wang WL, Song SH, Lu Z, Bosmans RP, Dai Q, Hao YJ, Yang X, Zhao WM, Tong WM, Wang XJ, Bogdan F, Furu K, Fu Y, Jia G, Zhao X, Liu J, Krokan HE, Klungland A, Yang YG, He C (January 2013). "ALKBH5 is a mammalian RNA demethylase that impacts RNA metabolism and mouse fertility". Molecular Cell. 49 (1): 18–29. doi:10.1016/j.molcel.2012.10.015. PMC . PMID 23177736.
- Wang X, Lu Z, Gomez A, Hon GC, Yue Y, Han D, Fu Y, Parisien M, Dai Q, Jia G, Ren B, Pan T, He C (January 2014). "N6-methyladenosine-dependent regulation of messenger RNA stability". Nature. 505 (7481): 117–20. doi:10.1038/nature12730. PMC . PMID 24284625.
- Wang X, Zhao BS, Roundtree IA, Lu Z, Han D, Ma H, Weng X, Chen K, Shi H, He C (June 2015). "N(6)-methyladenosine Modulates Messenger RNA Translation Efficiency". Cell. 161 (6): 1388–99. doi:10.1016/j.cell.2015.05.014. PMC . PMID 26046440.
- Xu C, Wang X, Liu K, Roundtree IA, Tempel W, Li Y, Lu Z, He C, Min J (November 2014). "Structural basis for selective binding of m6A RNA by the YTHDC1 YTH domain". Nature Chemical Biology. 10 (11): 927–9. doi:10.1038/nchembio.1654. PMID 25242552.
- Xiao W, Adhikari S, Dahal U, Chen YS, Hao YJ, Sun BF, Sun HY, Li A, Ping XL, Lai WY, Wang X, Ma HL, Huang CM, Yang Y, Huang N, Jiang GB, Wang HL, Zhou Q, Wang XJ, Zhao YL, Yang YG (February 2016). "Nuclear m(6)A Reader YTHDC1 Regulates mRNA Splicing". Molecular Cell. 61 (4): 507–19. doi:10.1016/j.molcel.2016.01.012. PMID 26876937.
- Luo S, Tong L (September 2014). "Molecular basis for the recognition of methylated adenines in RNA by the eukaryotic YTH domain". Proceedings of the National Academy of Sciences of the United States of America. 111 (38): 13834–9. doi:10.1073/pnas.1412742111. PMC . PMID 25201973.
- Zhu T, Roundtree IA, Wang P, Wang X, Wang L, Sun C, Tian Y, Li J, He C, Xu Y (December 2014). "Crystal structure of the YTH domain of YTHDF2 reveals mechanism for recognition of N6-methyladenosine". Cell Research. 24 (12): 1493–6. doi:10.1038/cr.2014.152. PMC . PMID 25412661.
- Liu N, Dai Q, Zheng G, He C, Parisien M, Pan T (February 2015). "N(6)-methyladenosine-dependent RNA structural switches regulate RNA-protein interactions". Nature. 518 (7540): 560–4. doi:10.1038/nature14234. PMC . PMID 25719671.
- Bodi Z, Zhong S, Mehra S, Song J, Graham N, Li H, May S, Fray RG (2012). "Adenosine Methylation in Arabidopsis mRNA is Associated with the 3' End and Reduced Levels Cause Developmental Defects". Frontiers in Plant Science. 3: 48. doi:10.3389/fpls.2012.00048. PMC . PMID 22639649.
- Sun WJ, Li JH, Liu S, Wu J, Zhou H, Qu LH, Yang JH (January 2016). "RMBase: a resource for decoding the landscape of RNA modifications from high-throughput sequencing data". Nucleic Acids Research. 44 (D1): D259–65. doi:10.1093/nar/gkv1036. PMID 26464443.
- Ke S, Alemu EA, Mertens C, Gantman EC, Fak JJ, Mele A, Haripal B, Zucker-Scharff I, Moore MJ, Park CY, Vågbø CB, Kusśnierczyk A, Klungland A, Darnell JE, Darnell RB (October 2015). "A majority of m6A residues are in the last exons, allowing the potential for 3' UTR regulation". Genes & Development. 29 (19): 2037–53. doi:10.1101/gad.269415.115. PMC . PMID 26404942.
- Fustin JM, Doi M, Yamaguchi Y, Hida H, Nishimura S, Yoshida M, Isagawa T, Morioka MS, Kakeya H, Manabe I, Okamura H (November 2013). "RNA-methylation-dependent RNA processing controls the speed of the circadian clock". Cell. 155 (4): 793–806. doi:10.1016/j.cell.2013.10.026. PMID 24209618.
- Hastings MH (November 2013). "m(6)A mRNA methylation: a new circadian pacesetter". Cell. 155 (4): 740–1. doi:10.1016/j.cell.2013.10.028. PMID 24209613.
- Akilzhanova A, Nurkina Z, Momynaliev K, Ramanculov E, Zhumadilov Z, Zhumadilov Z, Rakhypbekov T, Hayashida N, Nakashima M, Takamura N (September 2013). "Genetic profile and determinants of homocysteine levels in Kazakhstan patients with breast cancer". Anticancer Research. 33 (9): 4049–59. PMID 24023349.
- Reddy SM, Sadim M, Li J, Yi N, Agarwal S, Mantzoros CS, Kaklamani VG (August 2013). "Clinical and genetic predictors of weight gain in patients diagnosed with breast cancer". British Journal of Cancer. 109 (4): 872–81. doi:10.1038/bjc.2013.441. PMC . PMID 23922112.
- Heiliger KJ, Hess J, Vitagliano D, Salerno P, Braselmann H, Salvatore G, Ugolini C, Summerer I, Bogdanova T, Unger K, Thomas G, Santoro M, Zitzelsberger H (June 2012). "Novel candidate genes of thyroid tumourigenesis identified in Trk-T1 transgenic mice". Endocrine-Related Cancer. 19 (3): 409–21. doi:10.1530/ERC-11-0387. PMID 22454401.
- Ortega A, Niksic M, Bachi A, Wilm M, Sánchez L, Hastie N, Valcárcel J (January 2003). "Biochemical function of female-lethal (2)D/Wilms' tumor suppressor-1-associated proteins in alternative pre-mRNA splicing". The Journal of Biological Chemistry. 278 (5): 3040–7. doi:10.1074/jbc.M210737200. PMID 12444081.
- Jin DI, Lee SW, Han ME, Kim HJ, Seo SA, Hur GY, Jung S, Kim BS, Oh SO (December 2012). "Expression and roles of Wilms' tumor 1-associating protein in glioblastoma". Cancer Science. 103 (12): 2102–9. doi:10.1111/cas.12022. PMID 22957919.
- Lin Y, Ueda J, Yagyu K, Ishii H, Ueno M, Egawa N, Nakao H, Mori M, Matsuo K, Kikuchi S (July 2013). "Association between variations in the fat mass and obesity-associated gene and pancreatic cancer risk: a case-control study in Japan". BMC Cancer. 13: 337. doi:10.1186/1471-2407-13-337. PMC . PMID 23835106.
- Casalegno-Garduño R, Schmitt A, Wang X, Xu X, Schmitt M (October 2010). "Wilms' tumor 1 as a novel target for immunotherapy of leukemia". Transplantation Proceedings. 42 (8): 3309–11. doi:10.1016/j.transproceed.2010.07.034. PMID 20970678.
- Linnebacher M, Wienck A, Boeck I, Klar E (2010-03-18). "Identification of an MSI-H tumor-specific cytotoxic T cell epitope generated by the (-1) frame of U79260(FTO)". Journal of Biomedicine & Biotechnology. 2010: 841451. doi:10.1155/2010/841451. PMC . PMID 20339516.
- Machiela MJ, Lindström S, Allen NE, Haiman CA, Albanes D, Barricarte A, Berndt SI, Bueno-de-Mesquita HB, Chanock S, Gaziano JM, Gapstur SM, Giovannucci E, Henderson BE, Jacobs EJ, Kolonel LN, Krogh V, Ma J, Stampfer MJ, Stevens VL, Stram DO, Tjønneland A, Travis R, Willett WC, Hunter DJ, Le Marchand L, Kraft P (December 2012). "Association of type 2 diabetes susceptibility variants with advanced prostate cancer risk in the Breast and Prostate Cancer Cohort Consortium". American Journal of Epidemiology. 176 (12): 1121–9. doi:10.1093/aje/kws191. PMC . PMID 23193118.
- Long J, Zhang B, Signorello LB, Cai Q, Deming-Halverson S, Shrubsole MJ, Sanderson M, Dennis J, Michailidou K, Michailiou K, Easton DF, Shu XO, Blot WJ, Zheng W (2013-04-08). "Evaluating genome-wide association study-identified breast cancer risk variants in African-American women". PloS One. 8 (4): e58350. doi:10.1371/journal.pone.0058350. PMC . PMID 23593120.
- Kaklamani V, Yi N, Sadim M, Siziopikou K, Zhang K, Xu Y, Tofilon S, Agarwal S, Pasche B, Mantzoros C (April 2011). "The role of the fat mass and obesity associated gene (FTO) in breast cancer risk". BMC Medical Genetics. 12: 52. doi:10.1186/1471-2350-12-52. PMC . PMID 21489227.
- Pierce BL, Austin MA, Ahsan H (June 2011). "Association study of type 2 diabetes genetic susceptibility variants and risk of pancreatic cancer: an analysis of PanScan-I data". Cancer Causes & Control. 22 (6): 877–83. doi:10.1007/s10552-011-9760-5. PMID 21445555.
- Bokar, Joseph A. (2005-01-01). Grosjean, Henri, ed. Fine-Tuning of RNA Functions by Modification and Editing. Topics in Current Genetics. Springer Berlin Heidelberg. pp. 141–177. doi:10.1007/b106365. ISBN 9783540244950.
- Lin S, Choe J, Du P, Triboulet R, Gregory RI (May 2016). "The m(6)A Methyltransferase METTL3 Promotes Translation in Human Cancer Cells". Molecular Cell. 62 (3): 335–45. doi:10.1016/j.molcel.2016.03.021. PMC . PMID 27117702.
- Zhang C, Samanta D, Lu H, Bullen JW, Zhang H, Chen I, He X, Semenza GL (April 2016). "Hypoxia induces the breast cancer stem cell phenotype by HIF-dependent and ALKBH5-mediated m⁶A-demethylation of NANOG mRNA". Proceedings of the National Academy of Sciences of the United States of America. 113 (14): E2047–56. doi:10.1073/pnas.1602883113. PMC . PMID 27001847.
- Loos RJ, Yeo GS (January 2014). "The bigger picture of FTO: the first GWAS-identified obesity gene". Nature Reviews. Endocrinology. 10 (1): 51–61. doi:10.1038/nrendo.2013.227. PMC . PMID 24247219.
- Frayling TM, Timpson NJ, Weedon MN, Zeggini E, Freathy RM, Lindgren CM, Perry JR, Elliott KS, Lango H, Rayner NW, Shields B, Harries LW, Barrett JC, Ellard S, Groves CJ, Knight B, Patch AM, Ness AR, Ebrahim S, Lawlor DA, Ring SM, Ben-Shlomo Y, Jarvelin MR, Sovio U, Bennett AJ, Melzer D, Ferrucci L, Loos RJ, Barroso I, Wareham NJ, Karpe F, Owen KR, Cardon LR, Walker M, Hitman GA, Palmer CN, Doney AS, Morris AD, Smith GD, Hattersley AT, McCarthy MI (May 2007). "A common variant in the FTO gene is associated with body mass index and predisposes to childhood and adult obesity". Science. 316 (5826): 889–94. doi:10.1126/science.1141634. PMC . PMID 17434869.
- Wang L, Yu Q, Xiong Y, Liu L, Zhang X, Zhang Z, Wu J, Wang B. "Variant rs1421085 in the FTO gene contribute childhood obesity in Chinese children aged 3-6 years". Obesity Research & Clinical Practice. 7 (1): e14–22. doi:10.1016/j.orcp.2011.12.007. PMID 24331679.
- Kalnina I, Zaharenko L, Vaivade I, Rovite V, Nikitina-Zake L, Peculis R, Fridmanis D, Geldnere K, Jacobsson JA, Almen MS, Pirags V, Schiöth HB, Klovins J (September 2013). "Polymorphisms in FTO and near TMEM18 associate with type 2 diabetes and predispose to younger age at diagnosis of diabetes". Gene. 527 (2): 462–8. doi:10.1016/j.gene.2013.06.079. PMID 23860325.
- Karra E, O'Daly OG, Choudhury AI, Yousseif A, Millership S, Neary MT, Scott WR, Chandarana K, Manning S, Hess ME, Iwakura H, Akamizu T, Millet Q, Gelegen C, Drew ME, Rahman S, Emmanuel JJ, Williams SC, Rüther UU, Brüning JC, Withers DJ, Zelaya FO, Batterham RL (August 2013). "A link between FTO, ghrelin, and impaired brain food-cue responsivity". The Journal of Clinical Investigation. 123 (8): 3539–51. doi:10.1172/jci44403. PMID 23867619.
- Zhao X, Yang Y, Sun BF, Shi Y, Yang X, Xiao W, Hao YJ, Ping XL, Chen YS, Wang WJ, Jin KX, Wang X, Huang CM, Fu Y, Ge XM, Song SH, Jeong HS, Yanagisawa H, Niu Y, Jia GF, Wu W, Tong WM, Okamoto A, He C, Rendtlew Danielsen JM, Wang XJ, Yang YG (December 2014). "FTO-dependent demethylation of N6-methyladenosine regulates mRNA splicing and is required for adipogenesis". Cell Research. 24 (12): 1403–19. doi:10.1038/cr.2014.151. PMC . PMID 25412662.
- Merkestein M, Laber S, McMurray F, Andrew D, Sachse G, Sanderson J, Li M, Usher S, Sellayah D, Ashcroft FM, Cox RD (April 2015). "FTO influences adipogenesis by regulating mitotic clonal expansion". Nature Communications. 6: 6792. doi:10.1038/ncomms7792. PMC . PMID 25881961.
- Zhang M, Zhang Y, Ma J, Guo F, Cao Q, Zhang Y, Zhou B, Chai J, Zhao W, Zhao R (2015-07-28). "The Demethylase Activity of FTO (Fat Mass and Obesity Associated Protein) Is Required for Preadipocyte Differentiation". PloS One. 10 (7): e0133788. doi:10.1371/journal.pone.0133788. PMC . PMID 26218273.
- Hess ME, Hess S, Meyer KD, Verhagen LA, Koch L, Brönneke HS, Dietrich MO, Jordan SD, Saletore Y, Elemento O, Belgardt BF, Franz T, Horvath TL, Rüther U, Jaffrey SR, Kloppenburg P, Brüning JC (August 2013). "The fat mass and obesity associated gene (Fto) regulates activity of the dopaminergic midbrain circuitry". Nature Neuroscience. 16 (8): 1042–8. doi:10.1038/nn.3449. PMID 23817550.
- Kim HJ, Kim NC, Wang YD, Scarborough EA, Moore J, Diaz Z, MacLea KS, Freibaum B, Li S, Molliex A, Kanagaraj AP, Carter R, Boylan KB, Wojtas AM, Rademakers R, Pinkus JL, Greenberg SA, Trojanowski JQ, Traynor BJ, Smith BN, Topp S, Gkazi AS, Miller J, Shaw CE, Kottlors M, Kirschner J, Pestronk A, Li YR, Ford AF, Gitler AD, Benatar M, King OD, Kimonis VE, Ross ED, Weihl CC, Shorter J, Taylor JP (March 2013). "Mutations in prion-like domains in hnRNPA2B1 and hnRNPA1 cause multisystem proteinopathy and ALS". Nature. 495 (7442): 467–73. doi:10.1038/nature11922. PMC . PMID 23455423.
- Narayan, Prema; Rottman, Fritz M. (1992-01-01). Nord, F. F., ed. Advances in Enzymology and Related Areas of Molecular Biology. John Wiley & Sons, Inc. pp. 255–285. doi:10.1002/9780470123119.ch7/summary. ISBN 9780470123119.
- Kennedy EM, Bogerd HP, Kornepati AV, Kang D, Ghoshal D, Marshall JB, Poling BC, Tsai K, Gokhale NS, Horner SM, Cullen BR (May 2016). "Posttranscriptional m(6)A Editing of HIV-1 mRNAs Enhances Viral Gene Expression". Cell Host & Microbe. 19 (5): 675–85. doi:10.1016/j.chom.2016.04.002. PMC . PMID 27117054.
- Tirumuru N, Zhao BS, Lu W, Lu Z, He C, Wu L (July 2016). "N(6)-methyladenosine of HIV-1 RNA regulates viral infection and HIV-1 Gag protein expression". eLife. 5. doi:10.7554/eLife.15528. PMC . PMID 27371828.
- Lichinchi G, Gao S, Saletore Y, Gonzalez GM, Bansal V, Wang Y, Mason CE, Rana TM (February 2016). "Dynamics of the human and viral m(6)A RNA methylomes during HIV-1 infection of T cells". Nature Microbiology. 1 (4): 16011. doi:10.1038/nmicrobiol.2016.11. PMID 27572442.
- Lichinchi G, Zhao BS, Wu Y, Lu Z, Qin Y, He C, Rana TM (November 2016). "Dynamics of Human and Viral RNA Methylation during Zika Virus Infection". Cell Host & Microbe. 20 (5): 666–673. doi:10.1016/j.chom.2016.10.002. PMC . PMID 27773536.
- Gokhale NS, McIntyre AB, McFadden MJ, Roder AE, Kennedy EM, Gandara JA, Hopcraft SE, Quicke KM, Vazquez C, Willer J, Ilkayeva OR, Law BA, Holley CL, Garcia-Blanco MA, Evans MJ, Suthar MS, Bradrick SS, Mason CE, Horner SM (November 2016). "N6-Methyladenosine in Flaviviridae Viral RNA Genomes Regulates Infection". Cell Host & Microbe. 20 (5): 654–665. doi:10.1016/j.chom.2016.09.015. PMC . PMID 27773535.