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Protein NCK2 PDB 1u5s.png
Available structures
PDB Ortholog search: PDBe RCSB
Aliases NCK2, GRB4, NCKbeta, NCK adaptor protein 2
External IDs MGI: 1306821 HomoloGene: 20794 GeneCards: NCK2
Gene location (Human)
Chromosome 2 (human)
Chr. Chromosome 2 (human)[1]
Chromosome 2 (human)
Genomic location for NCK2
Genomic location for NCK2
Band 2q12.2 Start 105,744,897 bp[1]
End 105,894,274 bp[1]
Species Human Mouse
RefSeq (mRNA)



RefSeq (protein)



Location (UCSC) Chr 2: 105.74 – 105.89 Mb Chr 1: 43.44 – 43.57 Mb
PubMed search [3] [4]
View/Edit Human View/Edit Mouse

Cytoplasmic protein NCK2 (also known as NCK-beta and Grb4) is a protein that in humans is encoded by the NCK2 gene.[5][6][7]


NCK belongs to family of adaptor proteins,There are two mammalian NCK genes, NCK1 and NCK2. NCK1 is located in chromosome 3 and NCK2 is located in chromosome 2. The protein contains three SH3 domains and one SH2 domain. The protein has no known catalytic function but has been shown to bind and recruit various proteins involved in the regulation of receptor protein tyrosine kinases. It is through these regulatory activities that this protein is believed to be involved in cytoskeletal reorganization. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.[7]


NCK2 has been shown to interact with:


  1. ^ a b c GRCh38: Ensembl release 89: ENSG00000071051 - Ensembl, May 2017
  2. ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000066877 - Ensembl, May 2017
  3. ^ "Human PubMed Reference:". 
  4. ^ "Mouse PubMed Reference:". 
  5. ^ a b c Chen M, She H, Davis EM, Spicer CM, Kim L, Ren R, Le Beau MM, Li W (Sep 1998). "Identification of Nck family genes, chromosomal localization, expression, and signaling specificity". The Journal of Biological Chemistry. 273 (39): 25171–8. doi:10.1074/jbc.273.39.25171. PMID 9737977. 
  6. ^ Liu J, Li M, Ran X, Fan JS, Song J (Jun 2006). "Structural insight into the binding diversity between the human Nck2 SH3 domains and proline-rich proteins". Biochemistry. 45 (23): 7171–84. doi:10.1021/bi060091y. PMID 16752908. 
  7. ^ a b "Entrez Gene: NCK2 NCK adaptor protein 2". 
  8. ^ a b Tu Y, Li F, Wu C (Dec 1998). "Nck-2, a novel Src homology2/3-containing adaptor protein that interacts with the LIM-only protein PINCH and components of growth factor receptor kinase-signaling pathways". Molecular Biology of the Cell. 9 (12): 3367–82. doi:10.1091/mbc.9.12.3367. PMC 25640Freely accessible. PMID 9843575. 
  9. ^ a b Braverman LE, Quilliam LA (Feb 1999). "Identification of Grb4/Nckbeta, a src homology 2 and 3 domain-containing adapter protein having similar binding and biological properties to Nck". The Journal of Biological Chemistry. 274 (9): 5542–9. doi:10.1074/jbc.274.9.5542. PMID 10026169. 
  10. ^ Tu Y, Li F, Goicoechea S, Wu C (Mar 1999). "The LIM-only protein PINCH directly interacts with integrin-linked kinase and is recruited to integrin-rich sites in spreading cells". Molecular and Cellular Biology. 19 (3): 2425–34. doi:10.1128/mcb.19.3.2425. PMC 84035Freely accessible. PMID 10022929. 
  11. ^ Chen M, She H, Kim A, Woodley DT, Li W (Nov 2000). "Nckbeta adapter regulates actin polymerization in NIH 3T3 fibroblasts in response to platelet-derived growth factor bb". Molecular and Cellular Biology. 20 (21): 7867–80. doi:10.1128/mcb.20.21.7867-7880.2000. PMC 86398Freely accessible. PMID 11027258. 
  12. ^ Goicoechea SM, Tu Y, Hua Y, Chen K, Shen TL, Guan JL, Wu C (Jul 2002). "Nck-2 interacts with focal adhesion kinase and modulates cell motility". The International Journal of Biochemistry & Cell Biology. 34 (7): 791–805. doi:10.1016/s1357-2725(02)00002-x. PMID 11950595. 
  13. ^ Gil D, Schamel WW, Montoya M, Sánchez-Madrid F, Alarcón B (Jun 2002). "Recruitment of Nck by CD3 epsilon reveals a ligand-induced conformational change essential for T cell receptor signaling and synapse formation". Cell. 109 (7): 901–12. doi:10.1016/S0092-8674(02)00799-7. PMID 12110186. 
  14. ^ Suzuki S, Mizutani M, Suzuki K, Yamada M, Kojima M, Hatanaka H, Koizumi S (Jun 2002). "Brain-derived neurotrophic factor promotes interaction of the Nck2 adaptor protein with the TrkB tyrosine kinase receptor". Biochemical and Biophysical Research Communications. 294 (5): 1087–92. doi:10.1016/S0006-291X(02)00606-X. PMID 12074588. 

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