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NCK adaptor protein 2
Protein NCK2 PDB 1u5s.png
PDB rendering based on 1u5s.
Available structures
PDB Ortholog search: PDBe, RCSB
Symbols NCK2 ; GRB4; NCKbeta
External IDs OMIM604930 MGI1306821 HomoloGene20794 GeneCards: NCK2 Gene
Species Human Mouse
Entrez 8440 17974
Ensembl ENSG00000071051 ENSMUSG00000066877
UniProt O43639 O55033
RefSeq (mRNA) NM_001004720 NM_010879
RefSeq (protein) NP_001004720 NP_035009
Location (UCSC) Chr 2:
106.36 – 106.51 Mb
Chr 1:
43.45 – 43.57 Mb
PubMed search [1] [2]

Cytoplasmic protein NCK2 (also known as NCK-beta and Grb4) is a protein that in humans is encoded by the NCK2 gene.[1][2][3]

NCK belongs to family of adaptor proteins,There are two mammalian NCK genes, NCK1 and NCK2. NCK1 is located in chromosome 3 and NCK2 is located in chromosome 2. The protein contains three SH3 domains and one SH2 domain. The protein has no known catalytic function but has been shown to bind and recruit various proteins involved in the regulation of receptor protein tyrosine kinases. It is through these regulatory activities that this protein is believed to be involved in cytoskeletal reorganization. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.[3]


NCK2 has been shown to interact with LIMS1,[4][5] Epidermal growth factor receptor,[1][5][6] PDGFRB,[1][6][7] PTK2,[8] T-cell surface glycoprotein CD3 epsilon chain[9] and TrkB.[10]


  1. ^ a b c Chen M, She H, Davis EM, Spicer CM, Kim L, Ren R, Le Beau MM, Li W (October 1998). "Identification of Nck family genes, chromosomal localization, expression, and signaling specificity". J Biol Chem 273 (39): 25171–8. doi:10.1074/jbc.273.39.25171. PMID 9737977. 
  2. ^ Liu J, Li M, Ran X, Fan JS, Song J (June 2006). "Structural insight into the binding diversity between the human Nck2 SH3 domains and proline-rich proteins". Biochemistry 45 (23): 7171–84. doi:10.1021/bi060091y. PMID 16752908. 
  3. ^ a b "Entrez Gene: NCK2 NCK adaptor protein 2". 
  4. ^ Tu, Y; Li F; Goicoechea S; Wu C (March 1999). "The LIM-Only Protein PINCH Directly Interacts with Integrin-Linked Kinase and Is Recruited to Integrin-Rich Sites in Spreading Cells". Mol. Cell. Biol. (UNITED STATES) 19 (3): 2425–34. ISSN 0270-7306. PMC 84035. PMID 10022929. 
  5. ^ a b Tu, Y; Li F; Wu C (December 1998). "Nck-2, a Novel Src Homology2/3-containing Adaptor Protein That Interacts with the LIM-only Protein PINCH and Components of Growth Factor Receptor Kinase-signaling Pathways". Mol. Biol. Cell (UNITED STATES) 9 (12): 3367–82. doi:10.1091/mbc.9.12.3367. ISSN 1059-1524. PMC 25640. PMID 9843575. 
  6. ^ a b Braverman, L E; Quilliam L A (February 1999). "Identification of Grb4/Nckbeta, a src homology 2 and 3 domain-containing adapter protein having similar binding and biological properties to Nck". J. Biol. Chem. (UNITED STATES) 274 (9): 5542–9. doi:10.1074/jbc.274.9.5542. ISSN 0021-9258. PMID 10026169. 
  7. ^ Chen, M; She H; Kim A; Woodley D T; Li W (November 2000). "Nckβ Adapter Regulates Actin Polymerization in NIH 3T3 Fibroblasts in Response to Platelet-Derived Growth Factor bb". Mol. Cell. Biol. (UNITED STATES) 20 (21): 7867–80. doi:10.1128/MCB.20.21.7867-7880.2000. ISSN 0270-7306. PMC 86398. PMID 11027258. 
  8. ^ Goicoechea, Silvia M; Tu Yizeng; Hua Yun; Chen Ka; Shen Tang-Long; Guan Jun-Lin; Wu Chuanyue (July 2002). "Nck-2 interacts with focal adhesion kinase and modulates cell motility". Int. J. Biochem. Cell Biol. (England) 34 (7): 791–805. doi:10.1016/S1357-2725(02)00002-X. ISSN 1357-2725. PMID 11950595. 
  9. ^ Gil, Diana; Schamel Wolfgang W A, Montoya María, Sánchez-Madrid Francisco, Alarcón Balbino (June 2002). "Recruitment of Nck by CD3 epsilon reveals a ligand-induced conformational change essential for T cell receptor signaling and synapse formation". Cell (United States) 109 (7): 901–12. doi:10.1016/S0092-8674(02)00799-7. ISSN 0092-8674. PMID 12110186. 
  10. ^ Suzuki, Shingo; Mizutani Masaharu; Suzuki Kenji; Yamada Masashi; Kojima Masami; Hatanaka Hiroshi; Koizumi Shinichi (June 2002). "Brain-derived neurotrophic factor promotes interaction of the Nck2 adaptor protein with the TrkB tyrosine kinase receptor". Biochem. Biophys. Res. Commun. (United States) 294 (5): 1087–92. doi:10.1016/S0006-291X(02)00606-X. ISSN 0006-291X. PMID 12074588. 

Further reading[edit]

  • Liu, Zhifa (2013). "NCK2 is significantly associated with opiates addiction in African-origin men". Scientific World Journal. 2013: 24714–20. doi:10.1155/2013/748979. PMID 23533358. 

External links[edit]