Nucleoporin 153 (Nup153) is a protein which in humans is encoded by the NUP153 gene. It is an essential component of the basket of nuclear pore complexes (NPCs) in vertebrates, and required for the achoring of NPCs. It also acts as the docking site of an importing karyopherin. On the cytoplasmic side of the NPC, Nup358 fulfills an analogous role.
Nuclear pore complexes are extremely elaborate structures that mediate the regulated movement of macromolecules between the nucleus and cytoplasm. These complexes are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. Nucleoporins are pore complex-specific glycoproteins characterized by cytoplasmically oriented O-linked N-acetylglucosamine residues and numerous repeats of the pentapeptide sequence XFXFG.
Nucleoporin 153 has a mass of 153 kDA (hence its name). It is filamentous and it contains three distinct domains: a N-terminal region within which a pore targeting domain has been identified, a central region containing multiple zinc finger motifs, and a C-terminal region containing multiple XFXFG repeats.
McMorrow I, Bastos R, Horton H, Burke B (1994). "Sequence analysis of a cDNA encoding a human nuclear pore complex protein, hnup153.". Biochim. Biophys. Acta. 1217 (2): 219–23. doi:10.1016/0167-4781(94)90040-X. PMID8110839.
Kehlenbach RH, Gerace L (2000). "Phosphorylation of the nuclear transport machinery down-regulates nuclear protein import in vitro.". J. Biol. Chem. 275 (23): 17848–56. doi:10.1074/jbc.M001455200. PMID10749866.
Kutay U, Hartmann E, Treichel N, et al. (2001). "Identification of two novel RanGTP-binding proteins belonging to the importin beta superfamily.". J. Biol. Chem. 275 (51): 40163–8. doi:10.1074/jbc.M006242200. PMID11024021.
Dimaano C, Ball JR, Prunuske AJ, Ullman KS (2002). "RNA association defines a functionally conserved domain in the nuclear pore protein Nup153.". J. Biol. Chem. 276 (48): 45349–57. doi:10.1074/jbc.M102592200. PMID11567018.