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|Impact of nanotechnology|
Nanomedicine is the medical application of nanotechnology. Nanomedicine ranges from the medical applications of nanomaterials, to nanoelectronic biosensors, and even possible future applications of molecular nanotechnology. Current problems for nanomedicine involve understanding the issues related to toxicity and environmental impact of nanoscale materials (materials whose structure is on the scale of nanometers, i.e. billionths of a meter).
Nanomedicine research is receiving funding from the US National Institutes of Health. Of note is the funding in 2005 of a five-year plan to set up four nanomedicine centers. In April 2006, the journal Nature Materials estimated that 130 nanotech-based drugs and delivery systems were being developed worldwide.
- 1 Overview
- 2 Medical use of nanomaterials
- 3 Diagnostic and medical devices
- 4 Neuro-electronic interfaces
- 5 Medical applications of molecular nanotechnology
- 6 See also
- 7 References
- 8 External links
The biological and medical research communities have exploited the unique properties of nanomaterials for various applications (e.g., contrast agents for cell imaging and therapeutics for treating cancer). Terms such as biomedical nanotechnology, nanobiotechnology, and nanomedicine are used to describe this hybrid field. Functionalities can be added to nanomaterials by interfacing them with biological molecules or structures. The size of nanomaterials is similar to that of most biological molecules and structures; therefore, nanomaterials can be useful for both in vivo and in vitro biomedical research and applications. Thus far, the integration of nanomaterials with biology has led to the development of diagnostic devices, contrast agents, analytical tools, physical therapy applications, and drug delivery vehicles.
Nanomedicine seeks to deliver a valuable set of research tools and clinically useful devices in the near future. The National Nanotechnology Initiative expects new commercial applications in the pharmaceutical industry that may include advanced drug delivery systems, new therapies, and in vivo imaging. Neuro-electronic interfaces and other nanoelectronics-based sensors are another active goal of research. Further down the line, the speculative field of molecular nanotechnology believes that cell repair machines could revolutionize medicine and the medical field.
Nanomedicine is a large industry, with nanomedicine sales reaching $6.8 billion in 2004, and with over 200 companies and 38 products worldwide, a minimum of $3.8 billion in nanotechnology R&D is being invested every year. As the nanomedicine industry continues to grow, it is expected to have a significant impact on the economy.
Medical use of nanomaterials
Two forms of nanomedicine that have already been tested in mice and are awaiting human trials that will be using gold nanoshells to help diagnose and treat cancer, and using liposomes as vaccine adjuvants and as vehicles for drug transport. Similarly, drug detoxification is also another application for nanomedicine which has shown promising results in rats. A benefit of using nanoscale for medical technologies is that smaller devices are less invasive and can possibly be implanted inside the body, plus biochemical reaction times are much shorter. These devices are faster and more sensitive than typical drug delivery. Advances in Lipid nanotechnology was also instrumental in engineering medical nanodevices and novel drug delivery systems as well as in developing sensing applications.
Nanotechnology has provided the possibility of delivering drugs to specific cells using nanoparticles. The overall drug consumption and side-effects may be lowered significantly by depositing the active agent in the morbid region only and in no higher dose than needed. This highly selective approach would reduce costs and human suffering. An example can be found in dendrimers and nanoporous materials. Another example is to use block co-polymers, which form micelles for drug encapsulation. They could hold small drug molecules transporting them to the desired location. Another vision is based on small electromechanical systems; nanoelectromechanical systems are being investigated for the active release of drugs. Some potentially important applications include cancer treatment with iron nanoparticles or gold shells. Targeted drug delivery is intended to reduce the side effects of drugs with concomitant decreases in consumption and treatment expenses. The increased efficiency of delivery results in overall societal benefit by reducing the amount of drug needed in an equipotent preparation of said therapy, and thus reduced cost to the consumer.
Nanomedical approaches to drug delivery center on developing nanoscale particles or molecules to improve drug bioavailability. Bioavailability refers to the presence of drug molecules where they are needed in the body and where they will do the most good. Drug delivery focuses on maximizing bioavailability both at specific places in the body and over a period of time. This can potentially be achieved by molecular targeting by nanoengineered devices. It is all about targeting the molecules and delivering drugs with cell precision. More than $65 billion are wasted each year due to poor bioavailability. In vivo imaging is another area where tools and devices are being developed. Using nanoparticle contrast agents, images such as ultrasound and MRI have a favorable distribution and improved contrast. The new methods of nanoengineered materials that are being developed might be effective in treating illnesses and diseases such as cancer. What nanoscientists will be able to achieve in the future is beyond current imagination. This might be accomplished by self assembled biocompatible nanodevices that will detect, evaluate, treat and report to the clinical doctor automatically.
Drug delivery systems, lipid- or polymer-based nanoparticles, can be designed to improve the pharmacological and therapeutic properties of drugs. The strength of drug delivery systems is their ability to alter the pharmacokinetics and biodistribution of the drug. However, the pharmacokinetics and pharmacodynamics of nanomedicine is highly variable among different patients. When designed to avoid the body's defence mechanisms, nanoparticles have beneficial properties that can be used to improve drug delivery. Where larger particles would have been cleared from the body, cells take up these nanoparticles because of their size. Complex drug delivery mechanisms are being developed, including the ability to get drugs through cell membranes and into cell cytoplasm. Efficiency is important because many diseases depend upon processes within the cell and can only be impeded by drugs that make their way into the cell. Triggered response is one way for drug molecules to be used more efficiently. Drugs are placed in the body and only activate on encountering a particular signal. For example, a drug with poor solubility will be replaced by a drug delivery system where both hydrophilic and hydrophobic environments exist, improving the solubility. Also, a drug may cause tissue damage, but with drug delivery, regulated drug release can eliminate the problem. If a drug is cleared too quickly from the body, this could force a patient to use high doses, but with drug delivery systems clearance can be reduced by altering the pharmacokinetics of the drug. Poor biodistribution is a problem that can affect normal tissues through widespread distribution, but the particulates from drug delivery systems lower the volume of distribution and reduce the effect on non-target tissue. Potential nanodrugs will work by very specific and well-understood mechanisms; one of the major impacts of nanotechnology and nanoscience will be in leading development of completely new drugs with more useful behavior and less side effects. Polymeric nano-particles are a competing technology to lipidic (based mainly on Phospholipids) nano-particles. There is an additional risk of toxicity associated with polymers not widely studied or understood. This toxicity could include (but not limited to) hepatotoxicity, nephrotoxicity etc. and can have long term impacts not easily evaluated in short term in-vivo clinical trials either in animals or humans. Since the degradation of polymers to either their monomers or other degradation products in the body cannot be accurately predicted (unclear metabolic pathway), this is a real risk, specially in medicines intended for long term patient use. Even if the toxicity is ignored, there is additional hepatic load of metabolism, again an area of concern for long term medication. The major advantages of polymers is stability, lower cost and predictable characterisation, so the formulation chemists prefer this. However, in the patient's body this very stability (slow degradation) is a negative factor. Phospholipids on the other hand are membrane lipids (already present in the body and surrounding each cell), have a GRAS (Generally Recognised As Safe) status from FDA and are derived from natural sources without any complex chemistry involved. They are not metabolised but rather absorbed by the body and the degradation products are themselves nutrients (fats or micronutrients). It is greatly observed that[who?] nanoparticles are promising tools for the advancement of drug delivery, medical imaging, and as diagnostic sensors. However, the biodistribution of these nanoparticles is still imperfect due to the complex host's reactions to nano- and microsized materials and the difficulty in targeting specific organs in the body. Nevertheless, a lot of work is still ongoing to optimize and better understand the potential and limitations of nanoparticulate systems. For example, current research in the excretory systems of mice shows the ability of gold composites to selectively target certain organs based on their size and charge. These composites are encapsulated by a dendrimer and assigned a specific charge and size. Positively-charged gold nanoparticles were found to enter the kidneys while negatively-charged gold nanoparticles remained in the liver and spleen. It is suggested that the positive surface charge of the nanoparticle decreases the rate of opsonization of nanoparticles in the liver, thus affecting the excretory pathway. Even at a relatively small size of 5 nm, though, these particles can become compartmentalized in the peripheral tissues, and will therefore accumulate in the body over time. While advancement of research proves that targeting and distribution can be augmented by nanoparticles, the dangers of nanotoxicity become an important next step in further understanding of their medical uses.
Nanoparticles are also used to circumvent multidrug resistance (MDR) mechanisms. Mechanisms of MDR include decreased uptake of drugs, reduced intracellular drug concentration by activation of the efflux transporters, modifications in cellular pathways by altering cell cycle checkpoints, increased metabolism of drugs, induced emergency response genes to impair apoptotic pathways and altered DNA repair mechanisms.
Applications and reported research studies
- Abraxane, approved by the U.S. Food and Drug Administration (FDA) to treat breast cancer, non-small- cell lung cancer (NSCLC) and pancreatic cancer, is the nanoparticle albumin bound paclitaxel.
- Doxil was originally approved by the FDA for the use on HIV-related Kaposi's sarcoma. It is now being used to also treat ovarian cancer and multiple myeloma. The drug is encased in liposomes, which helps to extend the life of the drug that is being distributed. Liposomes are self-assembling, spherical, closed colloidal structures that are composed of lipid bilayers that surround an aqueous space. The liposomes also help to increase the functionality and it helps to decrease the damage that the drug does to the heart muscles specifically.
- In a mice study, scientists from Rice University and University of Texas MD Anderson Cancer Center reported enhanced effectiveness and reduced toxicity of an existing treatment for head and neck cancer when using the nanoparticles to deliver the drug. The hydrophilic carbonic clusters functionalized with polyethylene glycol or PEG-HCC are mixed with the chemotherapeutic drug paclitaxel (Taxol) and the epidermal growth factor receptor (EGFR) targeted Cetuximab and injected intravenously. They found the tumors were killed more effectively with radiation and the healthy tissue suffered less toxicity than without the nanotechnology drug delivery. The standard treatment contains Cremophor EL which allows the hydrophobic paclitaxel to be delivered intravenously. Replacing the toxic Cremophor with carbon nanoparticles eliminated its side effect and improved drug targeting which in turn required a lower dose of the toxic paclitaxel.
- Researchers at Case Western Reserve University reported using nanoparticle chain to deliver doxorubicin to breast cancer cells in a mice study. Three magnetic, iron-oxide nanospheres were chemically linked to one doxorubicin-loaded liposome and formed a 100 nm long nanoparticle chain. After the nanochains penetrated the tumor, radiofrequency field was generated that caused the magnetic nanoparticles to vibrate and rupture the liposome, dispersing the drug in its free form throughout the tumor. The result showed that the nano treatment was more effective in halting tumor growth than the standard treatment with doxorubicin. It is also less harmful to healthy cells since only 5% to 10% of the standard dose of doxorubicin were used.
- Nanoparticles made of polyethylene glycol (PEG) carrying payload of antibiotics at its core could swift charge thus allowing them to target bacterial infection more precisely inside the body, a group of MIT researchers reported. The nanoparticles, containing a sub-layer of pH sensitive chains of the amino acid histidine, carry a slightly negative charge when circulating in the blood stream, can evade detection and clearing by the immune system. When they encounter an infection site the particles gain a positive charge provoked by the slightly acidic environment at the infection sites, allowing them to bind to the negatively charged bacterial cell walls and release antibiotics at locally high concentration. This nano delivery system can potentially destroy bacteria even if it has developed resistance to antibiotics because of the targeted high dose and prolonged release of the drug. Although a lot of work is still needed, the researchers believe that it points to a new direction of using nanotechnology to treat infectious disease.
- Using the biomimetic strategy, researchers in the Harvard University Wyss Institute demonstrated in a mouse model that the drug coated nanoparticles can dissolve blood clots by selectively binding to the narrowed regions in the blood vessels – just like the platelets do. Aggregates of biodegradable nanoparticles coated with tissue plasminogen activator (tPA), each about the size of a platelet, were injected intravenously. In the region of vessel narrowing, shear stresses dissociate the aggregates and release the tPA-coated nanoparticles which bind and degrade the blood clots. By précised targeting and concentrating drug at the location of obstruction, the dose used is less than 1/50th of the normal dose. The nanotherapeutics will greatly reduce the severe side effect of bleeding, commonly found in standard treatment of thrombosis.
- An early phase clinical trial using the platform of ‘Minicell’ nanoparticle for drug delivery have been tested on patients with advanced and untreatable cancer. Built from the membranes of mutant bacteria, the minicells were loaded with paclitaxel and coated with cetuximab, antibodies that bind the epidermal growth factor receptor (EGFR) which is often overexpressed in a number of cancers, as a 'homing' device to the tumor cells. The tumor cells recognize the bacteria from which the minicells have been derived, regard it as invading microorganism and engulf it. Once inside, the payload of anti-cancer drug kills the tumor cells. Measured at 400 nanometers, the minicell is bigger than synthetic particles developed for drug delivery. The researchers indicated that this larger size gives the minicells a better profile in side-effects because the minicells will preferentially leak out of the porous blood vessels around the tumor cells and do not reach the liver, digestive system and skin. This Phase 1 clinical trial demonstrated that this treatment is well tolerated by the patients. As a platform technology, the minicell drug delivery system can be used to treat a number of different cancers with different anti-cancer drugs with the benefit of lower dose and less side-effects.
- Researchers at The Methodist Hospital Research Institute in Houston have created 'LeukoLike Vectors' or LLVs. LLVs are the drug-carrying silicon nanoparticles coated with the lipoprotein removed from the membranes of the white blood cells, leukocytes. The 'cloaked' nanoparticles were found to behave like leukocytes, are able to evade the body's immune system and survive much longer in vivo when studied in mice. The scientists expressed that LLVs are able to overcome a major hurdle in nanomedicine delivery by avoiding clearance in blood stream, crossing the biological barriers and localizing at target tissues due to its leukocyte like characters. Synthetic membranes are expected to develop in the future to replace harvest from the white blood cells due to the limited source of leukocyte.
- Inhalable nanocarriers were developed using a nanostructured lipid nanocarrier-based system (NLCS). The lipid nanoparticles contain the drug payload (doxorubicin or paclitaxel); two types of small interfering RNA (siRNA, targeted the BCL-2 mRNA to suppress nonpump cellular resistance, and MRP1 mRNA to suppress pump drug resistance); and the targeting molecules, an analog of luteinizing hormone-releasing hormone (LHRH) which homes in the overexpressed receptors of the cancer cells. The disease model tested was a mouse model of human lung cancer. Results showed enhanced antitumor activity and significant reduction of exposure of healthy organs when compared to the conventional intravenous treatment.
- Self-powered nanoparticles with a payload of osteoporosis drug are reported to be pulled toward the fresh bone crack site, powered by the ion gradients generated by leaked minerals (ions) from the bone, and deliver highly targeted drugs for healing. Using a model system of human tibia and femur, researchers from Pennsylvania State University and Boston University used nanoparticles of polylactic-coglycolic acid (widely used in medical devices) carrying sodium alendronate (an osteoporosis drug) for the in vitro experiments and found the 'nanotrucks' successfully carry the drug to the fresh crack of the human bone.
- Nanodiamond is diamond particle with a diameter of 2 to 8 nm and surface functional groups to bind various kinds of molecule. Clusters of the nanodiamonds in the range of 50-100 nm in diameter can be manufactured to trap drug inside and slowly release the payload. Experiments by researchers in Northwestern University on mouse models with chemotherapy-resistant liver and breast cancers show that the nanodiamonds with a normally lethal dose of doxorubicin significantly reduce the size of the tumors without toxic side effects expected from the drug if administered alone. The nanodiamond drug complex is found to have a much longer half-life in circulation without lowering the white blood cell count and, once inside the tumor, the nanoparticles are retained much longer. Additional studies using nanodiamonds include treating the triple-negative breast cancer with epirubicin  and glioblastoma with doxorubicin. Increase in survival rate with reduced side effects were observed in both the mouse models. UCLA reported that contact lens embedded with nanodiamonds at the size of 5 nm in diameter and loaded with the glaucoma drug timolol maleate allow controlled and sustained release of the medicine. The nanodiamonds were combined with the polymer chitosan which is cleaved when the lens make contact with the lysozyme in tears. The drug releases slowly in the eyes when the nanodiamond structure breaks down. 
- In regenerative medicine research, silica based nanoparticles are used to deliver molecules to transplanted cells derived from stem cells. Using a mouse model, scientists delivered two synthetic versions of neurotrophic growth factors locally using mesoporous nanoparticles to motor neurons derived from transplanted embryonic stem cells and reported improved long term survival and functional differentiation of the transplant in vivo.
- Nanoparticle with a liposomal core and a gel like shell carrying two different types of drug is demonstrated to shrink breast cancer tumor in a mouse model. Researchers in the North Carolina State University designed this nanoparticle, called Gelipo, to deliver both drugs simultaneously and maximize the efficiency of both. The cross-linked hyaluronic acid (HA) shell holds and protects the tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) while the core carries liposomes filled with doxorubicin embedded with peptide. HA preferentially concentrates in hyaluronidase (HAase) rich tumor environment and interacts with receptors on the cancer cell membranes. When the HAase degrades the gel shell of Gelipo, the TRAIL protein is released onto the cell membrane and triggers apoptosis (cell death). The core liposomes are internalized by the tumor cells into endosomes, the cellular transport system. The peptide breaks down the endosome and releases doxorubicin intracellularly, maximizes the efficacy while minimizes the toxic side effects.
Protein and peptide delivery
Protein and peptides exert multiple biological actions in the human body and they have been identified as showing great promise for treatment of various diseases and disorders. These macromolecules are called biopharmaceuticals. Targeted and/or controlled delivery of these biopharmaceuticals using nanomaterials like nanoparticles and Dendrimers is an emerging field called nanobiopharmaceutics, and these products are called nanobiopharmaceuticals.
Applications and reported research studies
- Nanoparticles delivering the myelin antigens were found to induce immune tolerance in a mouse model with relapsing multiple sclerosis. Biodegradable polystyrene microparticles coated with the myelin sheath peptides reset the mouse's immune system and prevent the disease from recurring or reduce the symptoms by halting the attack of the immune system to the protective myelin sheath coating the nerve fibers of the central nervous system. Team of researchers in Northwestern University indicated that this treatment method can potentially be used in other autoimmune diseases.
- Researchers at UCLA developed a nanoparticle system with water-soluble shell encapsulated a protein derived from bird anemia virus, apoptin. Apoptin selectively sends ‘self-destruct’ signal to tumor cells and trigger the programmed cell death (apoptosis) as it builds up in the nucleus, while leaving healthy cells unharmed. In the mouse model of human breast cancer, the treatment was found to significantly reduce the tumors’ growth. This new form of treatment resembles chemotherapy and gene therapy but without the risk of harming healthy cells, a common pitfall of chemotherapy, and no probability of genetic mutation that usually accompanies gene therapy.
- A nano-network of nanoparticles with a solid core of insulin, modified dextran and glucose oxidase was demonstrated to be able to regulate and maintain normal blood sugar level in a mouse model with Type 1 diabetes for up to 10 days. The nanoparticle is coated with either the positively charged chitosan (from shrimp shells) or negatively charged alginate (from seaweed). The opposite charges hold the nanoparticles together as a nano-network even after they are injected subcutaneously. The porous particles allow blood to make contact with the nanoparticle cores. When there is high blood sugar level, glucose oxidase is activated and breaks down the dextran into gluconic acid. The disintegration of the cores releases the insulin which controls the blood sugar level to the normal range.
The small size of nanoparticles endows them with properties that can be very useful in oncology, particularly in imaging. Quantum dots (nanoparticles with quantum confinement properties, such as size-tunable light emission), when used in conjunction with MRI (magnetic resonance imaging), can produce exceptional images of tumor sites. These nanoparticles are much brighter than organic dyes and only need one light source for excitation. This means that the use of fluorescent quantum dots could produce a higher contrast image and at a lower cost than today's organic dyes used as contrast media. The downside, however, is that quantum dots are usually made of quite toxic elements.
Another nanoproperty, high surface area to volume ratio, allows many functional groups to be attached to a nanoparticle, which can seek out and bind to certain tumor cells. Additionally, the small size of nanoparticles (10 to 100 nanometers), allows them to preferentially accumulate at tumor sites (because tumors lack an effective lymphatic drainage system). Research into multifunctional nanoparticles that would detect, image, and then proceed to treat a tumor is under way. A promising new cancer treatment that may one day replace radiation and chemotherapy is edging closer to human trials. Kanzius RF therapy attaches microscopic nanoparticles to cancer cells and then "cooks" tumors inside the body with radio waves that heat only the nanoparticles and the adjacent (cancerous) cells.
Sensor test chips containing thousands of nanowires, able to detect proteins and other biomarkers left behind by cancer cells, could enable the detection and diagnosis of cancer in the early stages from a few drops of a patient's blood.
The basic point to use drug delivery is based upon three facts: a) efficient encapsulation of the drugs, b) successful delivery of said drugs to the targeted region of the body, and c) successful release of that drug there.
Researchers at Rice University under Prof. Jennifer West, have demonstrated the use of 120 nm diameter nanoshells coated with gold to kill cancer tumors in mice. The nanoshells can be targeted to bond to cancerous cells by conjugating antibodies or peptides to the nanoshell surface. By irradiating the area of the tumor with an infrared laser, which passes through flesh without heating it, the gold is heated sufficiently to cause death to the cancer cells.
Limitations to conventional cancer chemotherapy include drug resistance, lack of selectivity, and lack of solubility. Nanoparticles have the potential to overcome these problems.
Nanoparticles of cadmium selenide (quantum dots) glow when exposed to ultraviolet light. When injected, they seep into cancer tumors. The surgeon can see the glowing tumor, and use it as a guide for more accurate tumor removal.
In photodynamic therapy, a particle is placed within the body and is illuminated with light from the outside. The light gets absorbed by the particle and if the particle is metal, energy from the light will heat the particle and surrounding tissue. Light may also be used to produce high energy oxygen molecules which will chemically react with and destroy most organic molecules that are next to them (like tumors). This therapy is appealing for many reasons. It does not leave a "toxic trail" of reactive molecules throughout the body (chemotherapy) because it is directed where only the light is shined and the particles exist. Photodynamic therapy has potential for a noninvasive procedure for dealing with diseases, growth and tumors.
Also, gold nanoparticles have the potential to join numerous therapeutic functions into a single platform, by targeting specific tumor cells, tissues and organs. Actually, Conde et al. reported the evaluation of the inflammatory response and therapeutic siRNA silencing via RGD-nanoparticles in a lung cancer mouse model. This study reported the use of siRNA/RGD gold nanoparticles capable of targeting tumor cells in two lung cancer xenograft mouse models, resulting in successful and significant c-Myc oncogene downregulation followed by tumor growth inhibition and prolonged survival of the animals. This delivery system can achieve translocation of siRNA duplexes directly into the tumour cell cytoplasm and accomplish successful silencing of an oncogene expression. Actually, RGD/siRNA-AuNPs can target preferentially and be taken up by tumor cells via integrin αvβ3-receptor-mediated endocytosis with no cytotoxicity, showing that can accumulate in tumor tissues overexpressing αvβ3 integrins and selectively delivered c-Myc siRNA to suppress tumor growth and angiogenesis.
At Rice University, a flesh welder is used to fuse two pieces of chicken meat into a single piece. The two pieces of chicken are placed together touching. A greenish liquid containing gold-coated nanoshells is dribbled along the seam. An infrared laser is traced along the seam, causing the two sides to weld together. This could solve the difficulties and blood leaks caused when the surgeon tries to restitch the arteries that have been cut during a kidney or heart transplant. The flesh welder could weld the artery perfectly.
Tracking movement can help determine how well drugs are being distributed or how substances are metabolized. It is difficult to track a small group of cells throughout the body, so scientists used to dye the cells. These dyes needed to be excited by light of a certain wavelength in order for them to light up. While different color dyes absorb different frequencies of light, there was a need for as many light sources as cells. A way around this problem is with luminescent tags. These tags are quantum dots attached to proteins that penetrate cell membranes. The dots can be random in size, can be made of bio-inert material, and they demonstrate the nanoscale property that color is size-dependent. As a result, sizes are selected so that the frequency of light used to make a group of quantum dots fluoresce is an even multiple of the frequency required to make another group incandesce. Then both groups can be lit with a single light source. They have also found a way to insert nanoparticles into the affected parts of the body so that those parts of the body will glow showing the tumor growth or shrinkage or also organ trouble.
Nanotechnology may be able to help reproduce or repair damaged tissue. “Tissue engineering” makes use of artificially stimulated cell proliferation by using suitable nanomaterial-based scaffolds and growth factors. For example, bones could be regrown on carbon nanotube scaffolds. Tissue engineering might replace today's conventional treatments like organ transplants or artificial implants. Advanced forms of tissue engineering may lead to life extension. Artificial bone composites are also being manufactured from nanocrystalline calcium phosphates.
Nanoparticles can be used in combination therapy for decreasing antibiotic resistance or for their antimicrobial properties. It has been shown that Zinc Oxide nanoparticles can decrease the antibiotic resistance and enhance the antibacterial activity of Ciprofloxacin against microorganism in Vitro. Nanoparticles can interfere with the different proteins which are interacting in the antibiotic resistance or pharmacologic mechanisms of drugs. Another example is Silver which has been used for the treatment of burns and chronic wounds. Antibacterial and antifungal properties of nanoparticles have widened their applications in medicine and dentistry.
Buckyballs have been investigated for the ability to "interrupt" the allergy/immune response by preventing mast cells (which cause allergic response) from releasing histamine into the blood and tissues, by binding to free radicals "dramatically better than any anti-oxidant currently available, such as vitamin E". In February 2013, University of Pennsylvania researchers developed a way to prevent nano particles from triggering an immune system response ; which could aid the delivery of medicinal nanoparticles some time in future. This is done by using the protein CD47, found on most mammalian cell membranes which binds to the SIRPa in macrophages; preventing an immune response from being triggered.
Nanotechnology is helping to advance the use of arthroscopes, which are pencil-sized devices that are used in surgeries with lights and cameras so surgeons can do the surgeries with smaller incisions. The smaller the incisions the faster the healing time which is better for the patients. It is also helping to find a way to make an arthroscope smaller than a strand of hair.
Diagnostic and medical devices
Nanotechnology-on-a-chip is one more dimension of lab-on-a-chip technology. Magnetic nanoparticles, bound to a suitable antibody, are used to label specific molecules, structures or microorganisms. Gold nanoparticles tagged with short segments of DNA can be used for detection of genetic sequence in a sample. Multicolor optical coding for biological assays has been achieved by embedding different-sized quantum dots into polymeric microbeads. Nanopore technology for analysis of nucleic acids converts strings of nucleotides directly into electronic signatures.
- C-dots (Cornell dots) are the smallest silica-based nanoparticles with the size <10 nm. The particles are infused with organic dye which will light up with fluorescence. Clinical trial is underway since 2011 to use the C-dots as diagnostic tool to assist surgeons to identify the location of tumor cells.
Nanotechnology is also opening up new opportunities in implantable delivery systems, which are often preferable to the use of injectable drugs, because the latter frequently display first-order kinetics (the blood concentration goes up rapidly, but drops exponentially over time). This rapid rise may cause difficulties with toxicity, and drug efficacy can diminish as the drug concentration falls below the targeted range.
Neuro-electronic interfacing is a visionary goal dealing with the construction of nanodevices that will permit computers to be joined and linked to the nervous system. This idea requires the building of a molecular structure that will permit control and detection of nerve impulses by an external computer. The computers will be able to interpret, register, and respond to signals the body gives off when it feels sensations. The demand for such structures is huge because many diseases involve the decay of the nervous system (ALS and multiple sclerosis). Also, many injuries and accidents may impair the nervous system resulting in dysfunctional systems and paraplegia. If computers could control the nervous system through neuro-electronic interface, problems that impair the system could be controlled so that effects of diseases and injuries could be overcome. Two considerations must be made when selecting the power source for such applications. They are refuelable and nonrefuelable strategies.
A refuelable strategy implies energy is refilled continuously or periodically with external sonic, chemical, tethered, magnetic, or biological electrical sources; A nanoscale enzymatic biofuel cell for self-powered nanotechnology devices have been developed that uses glucose from biofluids including human blood and watermelons.
A nonrefuelable strategy implies that all power is drawn from internal energy storage which would stop when all energy is drained. One limitation to this innovation is the fact that electrical interference is a possibility. Electric fields, Electromagnetic pulses (EMP), and stray fields from other in vivo electrical devices can all cause interference. Also, thick insulators are required to prevent electron leakage, and if high conductivity of the in vivo medium occurs there is a risk of sudden power loss and “shorting out.” Finally, thick wires are also needed to conduct substantial power levels without overheating. Little practical progress has been made even though research is happening. The wiring of the structure is extremely difficult because they must be positioned precisely in the nervous system so that it is able to monitor and respond to nervous signals. The structures that will provide the interface must also be compatible with the body's immune system so that they will remain unaffected in the body for a long time. In addition, the structures must also sense ionic currents and be able to cause currents to flow backward. While the potential for these structures is amazing, there is no timetable for when they will be available.
Medical applications of molecular nanotechnology
Molecular nanotechnology is a speculative subfield of nanotechnology regarding the possibility of engineering molecular assemblers, machines which could re-order matter at a molecular or atomic scale. Molecular nanotechnology is highly theoretical, seeking to anticipate what inventions nanotechnology might yield and to propose an agenda for future inquiry. The proposed elements of molecular nanotechnology, such as molecular assemblers and nanorobots are far beyond current capabilities.
The somewhat speculative claims about the possibility of using nanorobots in medicine, advocates say, would totally change the world of medicine once it is realized. Nanomedicine would make use of these nanorobots (e.g., Computational Genes), introduced into the body, to repair or detect damages and infections. According to Robert Freitas of the Institute for Molecular Manufacturing, a typical blood borne medical nanorobot would be between 0.5–3 micrometres in size, because that is the maximum size possible due to capillary passage requirement. Carbon could be the primary element used to build these nanorobots due to the inherent strength and other characteristics of some forms of carbon (diamond/fullerene composites), and nanorobots would be fabricated in desktop nanofactories specialized for this purpose.
Nanodevices could be observed at work inside the body using MRI, especially if their components were manufactured using mostly 13C atoms rather than the natural 12C isotope of carbon, since 13C has a nonzero nuclear magnetic moment. Medical nanodevices would first be injected into a human body, and would then go to work in a specific organ or tissue mass. The doctor will monitor the progress, and make certain that the nanodevices have gotten to the correct target treatment region. The doctor will also be able to scan a section of the body, and actually see the nanodevices congregated neatly around their target (a tumor mass, etc.) so that he or she can be sure that the procedure was successful.
Cell repair machines
||This article possibly contains original research. (January 2009)|
Using drugs and surgery, doctors can only encourage tissues to repair themselves. With molecular machines, there will be more direct repairs. Cell repair will utilize the same tasks that living systems already prove possible. Access to cells is possible because biologists can insert needles into cells without killing them. Thus, molecular machines are capable of entering the cell. Also, all specific biochemical interactions show that molecular systems can recognize other molecules by touch, build or rebuild every molecule in a cell, and can disassemble damaged molecules. Finally, cells that replicate prove that molecular systems can assemble every system found in a cell. Therefore, since nature has demonstrated the basic operations needed to perform molecular-level cell repair, in the future, nanomachine based systems will be built that are able to enter cells, sense differences from healthy ones and make modifications to the structure.
The healthcare possibilities of these cell repair machines are impressive. Comparable to the size of viruses or bacteria, their compact parts would allow them to be more complex. The early machines will be specialized. As they open and close cell membranes or travel through tissue and enter cells and viruses, machines will only be able to correct a single molecular disorder like DNA damage or enzyme deficiency. Later, cell repair machines will be programmed with more abilities with the help of advanced AI systems.
Nanocomputers will be needed to guide these machines. These computers will direct machines to examine, take apart, and rebuild damaged molecular structures. Repair machines will be able to repair whole cells by working structure by structure. Then by working cell by cell and tissue by tissue, whole organs can be repaired. Finally, by working organ by organ, health is restored to the body. Cells damaged to the point of inactivity can be repaired because of the ability of molecular machines to build cells from scratch. Therefore, cell repair machines will free medicine from reliance on self repair alone.
Nanonephrology is a branch of nanomedicine and nanotechnology that seeks to use nanomaterials and nanodevices for the diagnosis, therapy, and management of renal diseases. It includes the following goals:
- the study of kidney protein structures at the atomic level
- nano-imaging approaches to study cellular processes in kidney cells
- nanomedical treatments that utilize nanoparticles to treat various kidney diseases
Advances in Nanonephrology are expected to be based on discoveries in the above areas that can provide nano-scale information on the cellular molecular machinery involved in normal kidney processes and in pathological states. By understanding the physical and chemical properties of proteins and other macromolecules at the atomic level in various cells in the kidney, novel therapeutic approaches can be designed to combat major renal diseases. The nanoscale artificial kidney is a goal that many physicians dream of. Nanoscale engineering advances will permit programmable and controllable nano-scale robots to execute curative and reconstructive procedures in the human kidney at the cellular and molecular levels. Designing nanostructures compatible with the kidney cells and that can safely operate in vivo is also a future goal. The ability to direct events in a controlled fashion at the cellular nanolevel has the potential of significantly improving the lives of patients with kidney diseases.
- Nanomedicine, Volume I: Basic Capabilities, by Robert A. Freitas Jr. 1999, ISBN 1-57059-645-X
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