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Before working in Hollyoaks, Barber-Lane appeared in Emmerdale, Bodies, The Cops, and in a Yorkshire Building Society commercial. She has featured in Woman, Red and NOW magazines. Barber-Lane has also appeared in theatres such as The Dance House Theatre, The Courtyard Theatre and The Robert Powell Theatre. She has also performed at the Bridgewater Hall in Manchester.
She is also one of the many celebrities who have teamed up with Cancer Research UK to re-release "Girls Just Want to Have Fun". She is married to Emmerdale actor Liam Fox, who plays Dan Spencer. They met in 1997 and married on the 10 August 1999, however they split in 2015. They have two children together.
- Tansley, Janet (22 May 2008), "My Last Supper — Nicole Barber Lane", Liverpool Echo, retrieved 1 March 2010
- "Hitting the high notes: UK women and celebrities unite for charity record" (Press release). Cancer Research UK. 2 March 2010. Retrieved 28 April 2010.
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