|Transmission electron micrograph of Norwalk virus. The white bar = 50 nm|
|Group:||Group IV ((+)ssRNA)|
Norovirus, sometimes referred to as the winter vomiting bug in the UK and Ireland, is the most common cause of viral gastroenteritis in humans. It affects people of all ages. The virus is transmitted by fecally contaminated food or water, by person-to-person contact, and via aerosolization of vomited virus and subsequent contamination of surfaces. The annual number of diarrhea-associated events in outpatients is estimated at 7.7 million in industrialized countries, with 0.5 million hospitalizations, and 9.0 million hospitalizations in developing countries, with nearly 2 million deaths.
Norovirus infection is characterized by nausea, vomiting, watery diarrhea, abdominal pain, and in some cases, loss of taste. A person usually develops symptoms of gastroenteritis 12 to 48 hours after being exposed to norovirus. General lethargy, weakness, muscle aches, headaches, and low-grade fevers may occur. The disease is usually self-limiting, and severe illness is rare. Although having norovirus can be unpleasant, it is not usually dangerous and most who contract it make a full recovery within two to three days. On surfaces, norovirus is rapidly inactivated by either sufficient heating or by chlorine-based disinfectants and polyquaternary amines, but the virus is less susceptible to alcohols and detergents.
After infection, immunity to norovirus is usually incomplete and temporary, with one publication drawing the conclusion that protective immunity to the same strain of norovirus lasts for six months, but that all such immunity is gone after two years. Outbreaks of norovirus infection often occur in closed or semiclosed communities, such as long-term care facilities, overnight camps, hospitals, schools, prisons, clubs, dormitories, and cruise ships, where the infection spreads very rapidly either by person-to-person transmission or through contaminated food. Many norovirus outbreaks have been traced to food that was handled by one infected person.
The genus name Norovirus is derived from Norwalk virus, the only species of the genus. The species causes approximately 90% of epidemic nonbacterial outbreaks of gastroenteritis around the world, and may be responsible for 50% of all foodborne outbreaks of gastroenteritis in the United States.
- 1 Diagnosis
- 2 Virology
- 3 Life cycle
- 4 Pathophysiology
- 5 Treatment
- 6 Prevention
- 7 Epidemiology
- 8 Human genetics
- 9 History
- 10 See also
- 11 References
- 12 External links
Specific diagnosis of norovirus is routinely made by polymerase chain reaction (PCR) assays or quantitative PCR assays, which give results within a few hours. These assays are very sensitive and can detect as few as 10 virus particles.
Due to a lack of specific therapy, the need for expensive stool diagnostics is being questioned by experts if gastroenteritis by noroviruses has already been detected in the environment.
Noroviruses are transmitted directly from person to person (62–84% of all reported outbreaks) and indirectly via contaminated water and food. They are extremely contagious, and fewer than twenty virus particles can cause an infection (some research suggests as few as five). Transmission can be aerosolized when those stricken with the illness vomit, and can be aerosolized by a toilet flush when vomit or diarrhea is present; infection can follow eating food or breathing air near an episode of vomiting, even if cleaned up. The viruses continue to be shed after symptoms have subsided and shedding can still be detected many weeks after infection.
Vomiting, in particular, transmits infection effectively, and appears to allow airborne transmission. In one incident, a person who vomited spread infection right across a restaurant, suggesting that many unexplained cases of food poisoning may have their source in vomit. In December 1998, 126 people were dining at six tables; one woman vomited onto the floor. Staff quickly cleaned up, and people continued eating. Three days later others started falling ill; 52 people reported a range of symptoms, from fever and nausea to vomiting and diarrhea. The cause was not immediately identified. Researchers plotted the seating arrangement: more than 90% of the people at the same table as the sick woman later reported becoming ill. There was a direct correlation between the risk of infection of people at other tables and how close they were to the sick woman. More than 70% of the diners at an adjacent table fell ill; at a table on the other side of the restaurant, the attack rate was still 25%. The outbreak was attributed to a Norwalk-like virus (norovirus). Other cases of transmission by vomit were later identified.
In one outbreak at an international scout jamboree in the Netherlands, each person with gastroenteritis infected an average of 14 people before increased hygiene measures were put in place. Even after these new measures were enacted, an ill person still infected an average of 2.1 other people. A US CDC study of 11 outbreaks in New York State lists the suspected mode of transmission as person-to-person in seven outbreaks, foodborne in two, waterborne in one, and one unknown. The source of waterborne outbreaks may include water from municipal supplies, wells, recreational lakes, swimming pools and ice machines.
Shellfish and salad ingredients are the foods most often implicated in norovirus outbreaks. Ingestion of shellfish that have not been sufficiently heated (under 75 °C / 165 °F) poses a high risk for norovirus infection. Foods other than shellfish may be contaminated by infected food handlers.
Noroviruses (NoV) are a genetically diverse group of single-stranded positive-sense RNA, non-enveloped viruses belonging to the Caliciviridae family. According to the International Committee on Taxonomy of Viruses, the genus Norovirus has one species, which is called Norwalk virus. Serotypes, strains and isolates include:
- Norwalk virus;
- Hawaii virus;
- Snow Mountain virus;
- Mexico virus;
- Desert Shield virus;
- Southampton virus;
- Lordsdale virus;
- Wilkinson virus.
Noroviruses commonly isolated in cases of acute gastroenteritis belong to two genogroups: genogroup I (GI) includes Norwalk virus, Desert Shield virus and Southampton virus; and II (GII), which includes Bristol virus, Lordsdale virus, Toronto virus, Mexico virus, Hawaii virus and Snow Mountain virus.
Noroviruses can genetically be classified into five different genogroups (GI, GII, GIII, GIV, and GV), which can be further divided into different genetic clusters or genotypes. For example, genogroup II, the most prevalent human genogroup, presently contains 19 genotypes. Genogroups I, II and IV infect humans, whereas genogroup III infects bovine species, and genogroup V has recently been isolated in mice.
Most noroviruses that infect humans belong to genogroups GI and GII. Noroviruses from Genogroup II, genotype 4 (abbreviated as GII.4) account for the majority of adult outbreaks of gastroenteritis and often sweep across the globe. Recent examples include US95/96-US strain, associated with global outbreaks in the mid- to late-1990s; Farmington Hills virus associated with outbreaks in Europe and the United States in 2002 and in 2004; and Hunter virus which was associated with outbreaks in Europe, Japan and Australasia. In 2006, there was another large increase in NoV infection around the globe. Reports have shown a link between the expression of human histo-blood group antigens (HBGAs) and the susceptibility to norovirus infection. Studies have suggested the viral capsid of noroviruses may have evolved from selective pressure of human HBGAs.
A 2008 study suggests the protein MDA-5 may be the primary immune sensor that detects the presence of noroviruses in the body. Some people have common variations of the MDA-5 gene that could make them more susceptible to norovirus infection.
A 2010 study suggested a specific genetic version of norovirus (which would not be distinguishable from other types of the virus using standard viral antibody tests) interacts with a specific mutation in the ATG16L1 gene to help trigger symptomatic Crohn's disease in mice that have been subjected to a chemical that causes intestinal injury similar to the process in humans. (There are other similar ways for such diseases to happen like this, and this study in itself does not prove norovirus causes Crohn's in humans).
Viruses in Norovirus are non-enveloped, with icosahedral geometries. Capsid diameters vary widely, from 23–40 nm in diameter. The larger capsids (38–40 nm) exhibit T=3 symmetry and are composed of 180 VP1 proteins. Small capsids (23 nm) show T=1 symmetry, and are composed of 60 VP1 proteins. The virus particles demonstrate an amorphous surface structure when visualized using electron microscopy.
Noroviruses contain a linear, non-segmented, positive-sense RNA genome of approximately 7.5 kbp, encoding a large polyprotein which is cleaved into six smaller non-structural proteins (NS1/2 to NS7) by the viral 3C-like protease (NS6), a major structural protein (VP1) of about 58~60 kDa and a minor capsid protein (VP2).
The estimated mutation rate (1.21×10−2 to 1.41 ×10−2 substitutions per site per year) in this virus is high even compared with other RNA viruses.
|Genus||Structure||Symmetry||Capsid||Genomic arrangement||Genomic segmentation|
Viral replication is cytoplasmic. Entry into the host cell is achieved by attachment to host receptors, which mediates endocytosis. Replication follows the positive stranded RNA virus replication model. Positive stranded RNA virus transcription is the method of transcription. Translation takes place by leaky scanning and RNA termination-reinitiation. Humans and other mammals serve as the natural host. Transmission routes are fecal-oral and contamination.
|Genus||Host details||Tissue tropism||Entry details||Release details||Replication site||Assembly site||Transmission|
|Norovirus||Humans; mammals||Intestinal epithelium||Cell receptor endocytosis||Lysis||Cytoplasm||Cytoplasm||Oral-fecal|
When a person becomes infected with norovirus, the virus is replicated within the small intestine. After approximately one to two days, norovirus infection symptoms can appear. The principal symptom is acute gastroenteritis that develops between 12 and 48 hours after exposure, and lasts for 24–72 hours. The disease is usually self-limiting, and characterized by nausea, forceful vomiting, watery diarrhea, and abdominal pain, and in some cases, loss of taste. General lethargy, weakness, muscle aches, headache, coughs, and low-grade fever may occur.
Severe illness is rare; although people are frequently treated at the emergency ward, they are rarely admitted to the hospital. The number of deaths from norovirus in the United States is estimated to be around 300 each year, with most of these occurring in the very young, the elderly, and persons with weakened immune systems. Symptoms may become life-threatening in these groups if dehydration or electrolyte imbalance is ignored or not treated.
There is no specific medicine to treat people with norovirus illness. Norovirus infection cannot be treated with antibiotics because it is not a bacterial infection. Treatments aim to avoid complications such as dehydration from fluid loss caused by vomiting and diarrhea, and to mitigate symptoms using antiemetics and antidiarrheals.
Hand washing and disinfectants
Hand washing with soap and water is an effective method for reducing the transmission of norovirus pathogens. Alcohol rubs (≥62% ethanol) may be used as an adjunct, but are less effective than hand-washing, as norovirus lacks a lipid viral envelope. Surfaces where norovirus particles may be present can be sanitised with a solution of 1.5% to 7.5% of household bleach in water, or other disinfectants effective against norovirus.
In health care facilities
In health-care environments, the prevention of nosocomial infections involves routine and terminal cleaning. Nonflammable alcohol vapor in CO2 systems is used in health care environments where medical electronics would be adversely affected by aerosolized chlorine or other caustic compounds.
In 2011, the Centers for Disease Control and Prevention (CDC) published a clinical practice guideline addressing strategies for the prevention and control of norovirus gastroenteritis outbreaks in health-care settings. Based on a systematic review of published scientific studies, the guideline presents 51 specific evidence-based recommendations, which were organized into 12 categories: 1) patient cohorting and isolation precautions, 2) hand hygiene, 3) patient transfer and ward closure, 4) food handlers in healthcare, 5) diagnostics, 6) personal protective equipment, 7) environmental cleaning, 8) staff leave and policy, 9) visitors, 10) education, 11) active case-finding, and 12) communication and notification. The guideline also identifies eight high-priority recommendations, and suggests several areas in need of future research.
Ligocyte announced in 2007 that it was working on a vaccine and had started phase 1 trials. The company has since been taken over by Takeda. As of 2011[update], a monovalent nasal vaccine had completed phase I/II trials, while bivalent intramuscular and nasal vaccines were at earlier stages of development. The two vaccines rely on using a virus-like particle that is made of the norovirus capsid proteins in order to mimic the external structure of the virus. Since there is no RNA in this particle, it is incapable of reproducing and cannot cause an infection.
The norovirus can survive for long periods outside a human host depending on the surface and temperature conditions: it can stay for weeks on hard surfaces, and up to twelve days on contaminated fabrics, and it can survive for months, maybe even years in contaminated still water. A 2006 study found the virus remained on surfaces used for food preparation seven days after contamination.
Detection in food
Routine protocols to detect norovirus in clams and oysters by reverse transcription polymerase chain reaction are being employed by governmental laboratories such as the Food and Drug Administration (FDA) in the USA.
Norovirus causes about 18% of all cases of acute gastroenteritis worldwide. It is relatively common in developed countries and in low-mortality developing countries (20% and 19% respectively) compared to high-mortality developing countries (14%). Proportionately it causes more illness in people in the community or in hospital outpatients (24% and 20% respectively) as compared with hospital inpatients (17%) in whom other causes are more common.
Age and emergence of new norovirus strains do not appear to affect the proportion of gastroenteritis attributable to norovirus.
Norovirus is a common cause of epidemics of gastroenteritis on cruise ships. The US Centers for Disease Control and Prevention through its Vessel Sanitation Program record and investigate outbreaks of gastrointestinal illness—mostly caused by norovirus—on cruise ships with both a U.S. and foreign itinerary; there were 12 in 2015, and 10 from 1 January to 9 May 2016. An outbreak may affect over 25% of passengers, and a smaller proportion of crew members.
A non-functional fucosyltransferase FUT2 provides high protection from the most common norovirus GII.4. Functional FUT2 fucosyltransferase transfers a fucose sugar to the end of the Histo-blood group ABO(H) precursor in gastrointestinal cells and saliva glands. The ABH antigen produced is thought to act as receptors for human norovirus. Homozygous carriers of any nonsense mutation in the FUT2 gene are called non-secretors, as no ABH antigen is produced. Approximately 20% of Caucasians are non-secretors due to the G428A and C571T nonsense mutations in FUT2 and therefore have strong although not absolute protection from the norovirus GII.4. Non-secretors can still produce ABH antigens in erythrocytes, as the precursor is formed by FUT1. Some norovirus genotypes (GI.3) can infect non-secretors.
Epidemiological studies have shown that individuals with different ABH phenotypes are infected with NoV strains in a genotype-specific manner. GII.4 includes global epidemic strains and binds to more Histo-blood group antigens than other genogroups.
The norovirus was originally named the "Norwalk agent" after Norwalk, Ohio, in the United States, where an outbreak of acute gastroenteritis occurred among children at Bronson Elementary School in November 1968. In 1972, electron microscopy on stored human stool samples identified a virus, which was given the name "Norwalk virus". Numerous outbreaks with similar symptoms have been reported since. The cloning and sequencing of the Norwalk virus genome showed that these viruses have a genomic organization consistent with viruses belonging to the family Caliciviridae. The name "norovirus" (Norovirus for the genus) was approved by the International Committee on Taxonomy of Viruses (ICTV) in 2002. In 2011, however, a press release and a newsletter were published by ICTV, which strongly encouraged the media, national health authorities and the scientific community to use the virus name Norwalk virus, rather than the genus name Norovirus, when referring to outbreaks of the disease. This was also a public response by ICTV to the request from an individual in Japan to rename the Norovirus genus because of the possibility of negative associations for people in Japan and elsewhere who have the family name "Noro". Before this position of ICTV was made public, ICTV consulted widely with members of the Caliciviridae Study Group and carefully discussed the case.
In addition to "Norwalk agent" and "Norwalk virus", the virus has also been called "Norwalk-like virus", "small, round-structured viruses" (SRSVs), Spencer flu and "Snow Mountain virus". Common names of the illness caused by noroviruses still in use include "winter vomiting disease", "winter vomiting bug", "viral gastroenteritis", and "acute nonbacterial gastroenteritis". It also colloquially is known as "stomach flu", but this actually is a broad name that refers to gastric inflammation caused by a range of viruses and bacteria.
- Morillo SG, Timenetsky Mdo C (2011). "Norovirus: an overview". Revista Da Associação Médica Brasileira (1992). 57 (4): 453–8. doi:10.1016/s0104-4230(11)70094-x. PMID 21876931.
- Goodgame R (2006). "Norovirus gastroenteritis". Curr Gastroenterol Rep. 8 (5): 401–08. doi:10.1007/s11894-006-0026-4. PMID 16968608.
- Said MA, Perl TM, Sears CL (November 2008). "Healthcare epidemiology: gastrointestinal flu: norovirus in health care and long-term care facilities". Clinical Infectious Diseases. 47 (9): 1202–8. doi:10.1086/592299. PMID 18808354.
- Patel, MM; Widdowson, MA; Glass, RI; Akazawa, K; Vinjé, J; Parashar, UD (August 2008). "Systematic literature review of role of noroviruses in sporadic gastroenteritis.". Emerging infectious diseases. 14 (8): 1224–31. PMID 18680645.
- "Norovirus | Clinical Overview | CDC". www.cdc.gov. Retrieved 2016-03-28.
- "Norovirus - NHS Choices". Nhs.uk. 2014-01-20. Retrieved 2014-02-09.
- Jimenez L, Chiang M (2006). "Virucidal activity of a quaternary ammonium compound disinfectant against feline calicivirus: a surrogate for norovirus". Am J Infect Control. 34 (5): 269–73. doi:10.1016/j.ajic.2005.11.009. PMID 16765204.
- Lindesmith L, Moe C, Lependu J, Frelinger JA, Treanor J, Baric RS (2005). "Cellular and Humoral Immunity following Snow Mountain Virus Challenge". J. Virol. 79 (5): 2900–9. doi:10.1128/JVI.79.5.2900-2909.2005. PMC . PMID 15709009.
- Leon, Juan (2008). "Chapter 9". In Vajdy, Michael. Immunity Against Mucosal Pathogens. Springer. p. 232. ISBN 9781402084126.
- Noda M, Fukuda S, Nishio O (2007). "Statistical analysis of attack rate in norovirus foodborne outbreaks". Int J Food Microbiol. 122 (1–2): 216–20. doi:10.1016/j.ijfoodmicro.2007.11.073. PMID 18177970.
- Koopmans M, Duizer E (2004). "Foodborne viruses: an emerging problem". Int. J. Food Microbiol. 90 (1): 23–41. doi:10.1016/S0168-1605(03)00169-7. PMID 14672828.
- Lindesmith L, Moe C, Marionneau S, Ruvoen N, Jiang X, Lindblad L, Stewart P, LePendu J, Baric R (2003). "Human susceptibility and resistance to Norwalk virus infection". Nat. Med. 9 (5): 548–53. doi:10.1038/nm860. PMID 12692541.
- Widdowson MA, Sulka A, Bulens SN, Beard RS, Chaves SS, Hammond R, Salehi ED, Swanson E, Totaro J, Woron R, Mead PS, Bresee JS, Monroe SS, Glass RI (2005). "Norovirus and foodborne disease, United States, 1991–2000". Emerging Infect. Dis. 11 (1): 95–102. doi:10.3201/eid1101.040426. PMC . PMID 15705329.
- "Norovirus: Technical Fact Sheet". National Center for Infectious Diseases, CDC.
- Marshall JA, Bruggink LD (2006). "Laboratory diagnosis of norovirus". Clin. Lab. 52 (11–12): 571–81. PMID 17175887.
- Wilhelmi de Cal I, Revilla A, del Alamo JM, Román E, Moreno S, Sánchez-Fauquier A (2007). "Evaluation of two commercial enzyme immunoassays for the detection of norovirus in faecal samples from hospitalised children with sporadic acute gastroenteritis". Clin. Microbiol. Infect. 13 (3): 341–3. doi:10.1111/j.1469-0691.2006.01594.x. PMID 17391396.
- Fisman, David N.; Greer, Amy L.; Brouhanski, George; Drews, Steven J. (2009-01-01). "Of gastro and the gold standard: evaluation and policy implications of norovirus test performance for outbreak detection". Journal of Translational Medicine. 7: 23. doi:10.1186/1479-5876-7-23. ISSN 1479-5876. PMC . PMID 19323808.
- Moore MD, Goulter RM, Jaykus L (April 2015). "Human Norovirus as a Foodborne Pathogen: Challenges and Developments". Annual Review of Food Science and Technology. 6 (1): 411–33. doi:10.1146/annurev-food-022814-015643.
- Robert Matthews. "I've lost my appetite… - New Scientist". New Scientist. Retrieved 21 February 2016.
- Atmar RL, Opekun AR, Gilger MA, Estes MK, Crawford SE, Neill FH, Graham DY (October 2008). "Norwalk Virus Shedding after Experimental Human Infection". Emerging Infect. Dis. 14 (10): 1553–7. doi:10.3201/eid1410.080117. PMC . PMID 18826818.
- Marks PJ, Vipond IB, Carlisle D, Deakin D, Fey RE, Caul EO (June 2000). "Evidence for airborne transmission of Norwalk-like virus (NLV) in a hotel restaurant". Epidemiol. Infect. 124 (3): 481–487. doi:10.1017/s0950268899003805. PMC . PMID 10982072.
- Marks PJ, Vipond IB, Regan FM, Wedgwood K, Fey RE, Caul EO (Aug 2003). "A school outbreak of Norwalk-like virus: evidence for airborne transmission". Epidemiol. Infect. 131 (1): 727–736. doi:10.1017/s0950268803008689. PMC . PMID 12948373.
- Heijne JC, Teunis P, Morroy G, Wijkmans C, Oostveen S, Duizer E, Kretzschmar M, Wallinga J (2009). "Enhanced Hygiene Measures and Norovirus Transmission during an Outbreak" (PDF). Emerg. Infect. Dis. 15 (1): 24–30. doi:10.3201/eid1501.080299. PMC . PMID 19116045.
- Hedberg CW, Osterholm MT (1993). "Outbreaks of food-borne and waterborne viral gastroenteritis". Clin. Microbiol. Rev. 6 (3): 199–210. PMC . PMID 8395330.
- "Safe Internal Cooking Temperatures Chart". www.healthycanadians.gc.ca. Government of Canada. 2015-05-07.
- "[ARCHIVED CONTENT] HPA - Shellfish consumption and the risk of norovirus infection". Archived from the original on 14 July 2014. Retrieved 21 February 2016.
- Parashar UD, Monroe SS (2001). ""Norwalk-like viruses" as a cause of foodborne disease outbreaks". Rev. Med. Virol. 11 (4): 243–52. doi:10.1002/rmv.321. PMID 11479930.
- Department of Health and Ageing Norovirus laboratory case definition
- Eric B. Carstens; King, Andrew; Elliot Lefkowitz; Adams, Michael Ian (2011). Virus Taxonomy: Ninth Report of the International Committee on Taxonomy of Viruses. Amsterdam: Elsevier. pp. 981–982. ISBN 0-12-384684-6.
- Schuffenecker I, Ando T, Thouvenot D, Lina B, Aymard M (2001). "Genetic classification of "Sapporo-like viruses"". Arch. Virol. 146 (11): 2115–32. doi:10.1007/s007050170024. PMID 11765915.
- Ramirez S, Giammanco GM, De Grazia S, Colomba C, Martella V, Arista S (2008). "Genotyping of GII.4 and GIIb norovirus RT-PCR amplicons by RFLP analysis". J. Virol. Methods. 147 (2): 250–6. doi:10.1016/j.jviromet.2007.09.005. PMID 17953996.
- Vinjé J, Green J, Lewis DC, Gallimore CI, Brown DW, Koopmans MP (2000). "Genetic polymorphism across regions of the three open reading frames of "Norwalk-like viruses"". Arch. Virol. 145 (2): 223–41. doi:10.1007/s007050050020. PMID 10752550.
- Noel JS, Fankhauser RL, Ando T, Monroe SS, Glass RI (2000). "Identification of a distinct common strain of "Norwalk-like viruses" having a global distribution". J. Infect. Dis. 179 (6): 1334–44. doi:10.1086/314783. PMID 10228052.
- Tu ET, Bull RA, Greening GE, Hewitt J, Lyon MJ, Marshall JA, McIver CJ, Rawlinson WD, White PA (2008). "Epidemics of gastroenteritis during 2006 were associated with the spread of norovirus GII.4 variants 2006a and 2006b". Clin. Infect. Dis. 46 (3): 413–20. doi:10.1086/525259. PMID 18177226.
- Shirato H (2011). "Norovirus and histo-blood group antigens". Japanese Journal of Infectious Diseases. 64 (2): 95–103. PMID 21519121.
- McCartney SA, Thackray LB, Gitlin L, Gilfillan S, Virgin HW, Virgin Iv HW, Colonna M (July 18, 2008). Baric RS, ed. "MDA-5 Recognition of a Murine Norovirus". PLoS Pathog. 4 (7): e1000108. doi:10.1371/journal.ppat.1000108. PMC . PMID 18636103.
- Researchers Discover Primary Sensor That Detects Stomach Viruses Newswise, Retrieved on July 20, 2008.
- "Viral Zone". ExPASy. Retrieved 15 June 2015.
- Prasad BV, Crawford S, Lawton JA, Pesavento J, Hardy M, Estes MK (2001). "Structural studies on gastroenteritis viruses". Novartis Found. Symp. Novartis Foundation Symposia. 238: 26–37; discussion 37–46. doi:10.1002/0470846534.ch3. ISBN 978-0-470-84653-7. PMID 11444031.
- Thorne, Lucy G.; Goodfellow, Ian G. (2014-02-01). "Norovirus gene expression and replication". The Journal of General Virology. 95 (Pt 2): 278–291. doi:10.1099/vir.0.059634-0. ISSN 1465-2099. PMID 24243731.
- Clarke IN, Lambden PR (2000). "Organization and expression of calicivirus genes". J. Infect. Dis. 181 Suppl 2: S309–16. doi:10.1086/315575. PMID 10804143.
- Tan M, Hegde RS, Jiang X (2004). "The P Domain of Norovirus Capsid Protein Forms Dimer and Binds to Histo-Blood Group Antigen Receptors". J. Virol. 78 (12): 6233–42. doi:10.1128/JVI.78.12.6233-6242.2004. PMC . PMID 15163716.
- Tan M, Huang PW, Meller J, Zhong WM, Farkas T, Jiang X (2004). "Mutations within the P2 domain of norovirus capsid affect binding to human histo-blood group antigens: evidence for a binding pocket". J. Virol. 78 (6): 3201. doi:10.1128/JVI.78.6.3201.2004.
- Cao S, Lou Z, Tan M, Chen Y, Liu Y, Zhang Z, Zhang XC, Jiang X, Li X, Rao Z (2007). "Structural Basis for the Recognition of Blood Group Trisaccharides by Norovirus". J. Virol. 81 (11): 5949–57. doi:10.1128/JVI.00219-07. PMC . PMID 17392366.
- Lundborg M, Ali E, Widmalm G (2013). "An in silico virtual screening study for the design of norovirus inhibitors: fragment-based molecular docking and binding free energy calculations". Carbohydr Res. 378: 133–8. doi:10.1016/j.carres.2013.03.012. PMID 23582100.
- Ali ES, Rajapaksha H, Jillian MC, Petrovsky N (2016). "Norovirus drug candidates that inhibit viral capsid attachment to human histo-blood group antigens". Antiviral Res. 133: 14–22. doi:10.1016/j.antiviral.2016.07.006. PMC . PMID 27421712.
- Victoria M, Miagostovich MP, Ferreira MS, Vieira CB, Fioretti JM, Leite JP, Colina R, Cristina J (2009). "Bayesian coalescent inference reveals high evolutionary rates and expansion of Norovirus populations". Infect Genet Evol. 9 (5): 927–932. doi:10.1016/j.meegid.2009.06.014. PMID 19559104.
- "Norovirus - Treatment - CDC". Retrieved 21 February 2016.
- "Traveler's Diarrhea". Merck Manuals Consumer Version. Retrieved 21 February 2016.
- "List G: EPA Registered Hospital Disinfectants Effective Against Norovirus (Norwalk-like virus)". US Environmental Protection Agency. Retrieved 9 May 2016.
- "Gastroenteritis and Noroviruses—Dr Jim Grey, Health Protection Agency". The Naked Scientists. 2007-12-09. Retrieved 2014-02-09.
- Chadwick PR, Beards G, Brown D, Caul EO, Cheesbrough J, Clarke I, Curry A, O'Brien S, Quigley K, Sellwood J, Westmoreland D (2000). "Management of hospital outbreaks of gastroenteritis due to small roundstructured viruses". J. Hosp. Infect. 45 (1): 1–10. doi:10.1053/jhin.2000.0662. PMID 10833336.
- HICPAC. "Guideline for the Prevention and Control of Norovirus Gastroenteritis Outbreaks in Healthcare Settings, 2011". Healthcare Infection Control Practices Advisory Committee (HICPAC). Centers for Disease Control and Prevention (CDC). Retrieved 19 March 2015.
- MacCannell, T; Umscheid, CA; Agarwal, RK; Lee, I; Kuntz, G; Stevenson, KB (2011). "Guideline for the prevention and control of norovirus gastroenteritis outbreaks in healthcare settings". Infection Control and Hospital Epidemiology. 32 (10): 939–969. doi:10.1086/662025. PMID 21931246.
- "Norovirus Vaccine" (PDF).
- "Takeda to Acquire LigoCyte Pharmaceuticals, Inc.".
- Norovirus; Ligocyte.com; accessed .
- "How To Stay Well (When Everyone Else Is Sick)". Webmd.com. Retrieved 2014-02-09.
- Frazer, J. (January 17, 2012). "Misery-inducing Norovirus Can Survive for Months—Perhaps Years—in Drinking Water". Scientific American. Retrieved February 27, 2012.
- D'Souza DH, Sair A, Williams K, Papafragkou E, Jean J, Moore C, Jaykus L (2006). "Persistence of caliciviruses on environmental surfaces and their transfer to food". International Journal of Food Microbiology. 108 (1): 84–91. doi:10.1016/j.ijfoodmicro.2005.10.024. PMID 16473426.
- Shieh Y, Monroe SS, Fankhauser RL, Langlois GW, Burkhardt W, Baric RS (2000). "Detection of norwalk-like virus in shellfish implicated in illness". J. Infect. Dis. 181 (Suppl 2): S360–6. doi:10.1086/315578. PMID 10804149.
- "Winter vomiting bug cases up 40 per cent: Health Protection Agency". Telegraph.co.uk. 24 November 2012. Retrieved 21 February 2016.
- Ahmed SM, Hall AJ, Robinson AE, et al. (August 2014). "Global prevalence of norovirus in cases of gastroenteritis: a systematic review and meta-analysis". Lancet Infect Dis. 14 (8): 725–30. doi:10.1016/S1473-3099(14)70767-4. PMID 24981041.
- CDC VSP. "Vessel Sanitation Program - Outbreak Updates for International Cruise Ships". Centers for Disease Control and Prevention. Retrieved 9 May 2016.
- "CDC - Vessel Sanitation Program - Balmoral, April 16, 2016". Cdc.gov. Retrieved 9 May 2016.
- Carlsson B, Kindberg E, Buesa J, Rydell GE, Lidón MF, Montava R, Abu Mallouh R, Grahn A, Rodríguez-Díaz J, Bellido J, Arnedo A, Larson G, Svensson L (May 2009). "The G428A Nonsense Mutation in FUT2 Provides Strong but Not Absolute Protection against Symptomatic GII.4 Norovirus Infection". PLoS ONE. 4 (5): e5593. Bibcode:2009PLoSO...4.5593C. doi:10.1371/journal.pone.0005593. PMC . PMID 19440360.
- Rydell GE, Kindberg E, Larson G, Svensson L (November 2011). "Susceptibility to winter vomiting disease: a sweet matter". Rev. Med. Virol. 21 (6): 370–82. doi:10.1002/rmv.704. PMID 22025362.
- Shirato H (2011). "Norovirus and histo-blood group antigens". Jpn. J. Infect. Dis. 64 (2): 95–103. PMID 21519121.
- Nordgren J, Kindberg E, Lindgren PE, Matussek A, Svensson L (January 2010). "Norovirus gastroenteritis outbreak with a secretor-independent susceptibility pattern, Sweden". Emerg. Infect. Dis. 16 (1): 81–7. doi:10.3201/eid1601.090633. PMC . PMID 20031047.
- Hennessy EP, Green AD, Connor MP, Darby R, MacDonald P (July 2003). "Norwalk virus infection and disease is associated with ABO histo-blood group type". J. Infect. Dis. 188 (1): 176–7. doi:10.1086/375829. PMID 12825190.
- Le Guyader FS, Krol J, Ambert-Balay K, Ruvoen-Clouet N, Desaubliaux B, Parnaudeau S, Le Saux JC, Ponge A, Pothier P, Atmar RL, Le Pendu J (March 2010). "Comprehensive Analysis of a Norovirus-Associated Gastroenteritis Outbreak, from the Environment to the Consumer". Journal of Clinical Microbiology. 48 (3): 915–20. doi:10.1128/JCM.01664-09. PMC . PMID 20053852.
- Kapikian AZ (1996). "Overview of viral gastroenteritis". Arch. Virol. Suppl. 12: 7–19. PMID 9015097.
- ICTVdB Management (2006). 00.012.0.03. Norovirus. In: ICTVdB—The Universal Virus Database, version 4. Büchen-Osmond, C. (Ed), Columbia University, New York, USA
- "2011 ICTV Newsletter #9, November 2011". ICTV. November 14, 2011.
- Appleton H (1987). "Small round viruses: classification and role in food-borne infections ...". Ciba Found. Symp. 128: 108–25. PMID 3036438.
- "'Norwalk-Like Viruses' Public Health Consequences and Outbreak Management". Morbidity and Mortality Weekly Reports—Recommendations and Reports. US CDC. 50 (RR-9): 1–18. 2001. Retrieved 14 February 2016.
- "Norovirus shuts wards and unit at three Sussex hospitals". BBC News. January 11, 2012. Retrieved January 20, 2012.
- "Norovirus at Norfolk hospitals: Disruption continues". BBC News. January 12, 2012. Retrieved January 20, 2012.
- NHS Choices Health A–Z Norovirus infections
- Global network and database noroviruses
- CDC Viral Gastroenteritis FAQs: Center for Disease Control and Prevention of Food Illness Fact Sheet
- "Norovirus in Healthcare Facilities Fact Sheet", CDC, released December 21, 2006
- tips from CDC for cruise vacationers
- Virus Pathogen Database and Analysis Resource (ViPR): Caliciviridae
- 3D macromolecular structures of Noroviruses from the EM Data Bank(EMDB)
- Viralzone: Norovirus