Route 320 is a collector road in the Canadian province of Nova Scotia.
It is located in Richmond County and connects Louisdale at Exit 26 on Highway 104 with Arichat at Route 206 .
It crosses the Lennox Passage using the Burnt Island Bridge (a drawbridge) that connects Cape Breton Island to Isle Madame.
- Pondville Provincial Park
- Lennox Passage Provincial Park
The section of Collector Highway 320 from Arichat to Harbourview Crescent was once designated as Trunk Highway 20.