Novy-MacNeal-Nicolle medium

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Novy-MacNeal-Nicolle medium (NNN) is a culture medium used to grow Leishmania – needed when the amastigotes are not found by scraping. It consists of 0.6% sodium chloride (NaCl) added to a simple blood agar slope.[1] NNN can also be used to grow Trypanosoma cruzi.


  1. ^ Dawes, Ben (1967). Advances in parasitology, Volume 5. New York: Academic Press Inc.