Nucleic acid templated chemistry

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The schematic presentation how the nucleic acid templated chemistry works within cells
Schematic presentation of chemical reaction within cells to combine two precursors into an active drug

Nucleic acid templated chemistry (NATC), or DNA-templated chemistry, is a tool used in the controlled synthesis of chemical compounds. The main advantage of NAT-chemistry (NATC) is that it allows one to perform the chemical reaction as an intramolecular reaction. Two oligonucleotides or their analogues are linked via chemical groups to precursors of chemical compounds. The oligonucleotides recognize specific nucleic acids and are hybridized sterically close to each other. Afterwards the chemical active groups interact with each other to combine the precursors into a completely new chemical compound. NATC is usually used to perform synthesis of complex compounds without need to protect chemically active groups during the synthesis.

In 1999 Pavel Sergeev suggested the use of NATC to synthesize biologically active compounds within living organisms.,[1] including use within human cells. In this application, the precursors are distributed in the whole human body and the chemical reactions are performed only within cells having specific RNA molecules. This approach allows very specific synthesis within peculiar tissues or within specific cells of the tissue. It is especially a new tool to deliver medications to cancer cells. Additionally biologically active compounds could be delivered to specific cells within humans to promote the targeted cells to divisions. NATC also opens the possibility to treat bacterial diseases. Many scientific groups performed NATC in vivo to visualize eucaryotic as well as bacterial cells. As a principle it opens new perspectives to treat oncological and bacterial diseases as well as to visualize them.[2][3][4][5][6][7]

See also[edit]


  1. ^ Sergeev, Pavel, Patent application, WO200061775, filling date 8 April 1999 "Synthesis of biologically active compounds in cells" PCT/IB1999/000616.
  2. ^ Franzini RM, Kool ET (November 2009). "Efficient nucleic acid detection by templated reductive quencher release". J Am Chem Soc. 131 (44): 16021–16023. doi:10.1021/ja904138v. PMC 2774910. PMID 19886694.
  3. ^ Kleiner RE, Brudno Y, Birnbaum ME, Liu DR (April 2008). "DNA-templated polymerization of side-chain-functionalized peptide nucleic acid aldehydes". J. Am. Chem. Soc. 130 (14): 4646–4652. doi:10.1021/ja0753997. PMC 2748799. PMID 18341334.
  4. ^ Snyder TM, Tse BN, Liu DR (January 2008). "Effects of Template Sequence and Secondary Structure on DNA-Templated Reactivity". J. Am. Chem. Soc. 130 (4): 1392–1401. doi:10.1021/ja076780u. PMC 2533274. PMID 18179216.
  5. ^ Miller GP, Silver AP, Kool ET (January 2008). "New, Stronger Nucleophiles for Nucleic Acid-Templated Chemistry: Synthesis and Application in Fluorescence Detection of Cellular RNA". Bioorg. Med. Chem. 16 (1): 56–64. doi:10.1016/j.bmc.2007.04.051. PMC 2265789. PMID 17502150.
  6. ^ Gorska K, Huang KT, Chaloin O, Winssinger N (April 2009). "DNA-templated homo- and heterodimerization of peptide nucleic acid encoded oligosaccharides that mimick the carbohydrate epitope of HIV". Angew. Chem. Int. Ed. Engl. 48 (41): 7695–7700. doi:10.1002/anie.200903328. PMID 19774579. Archived from the original on 5 January 2013.
  7. ^ Pianowski Z, Gorska K, Oswald L, Merten CA, Winssinger N (May 2009). "Imaging of mRNA in live cells using nucleic acid-templated reduction of azidorhodamine probes". J. Am. Chem. Soc. 131 (19): 6492–6497. doi:10.1021/ja809656k. PMID 19378999.