|Informal group:||Paenarthrobacter ureafaciens KI72|
GTDB r95 & NCBI, 2020 (Busse HJ, 2016)
(Due to an OCR error, the strain name has occasionally been reported as "K172".)
Paenarthrobacter ureafaciens KI72, popularly known as nylon-eating bacteria, is a strain of Paenarthrobacter ureafaciens that can digest certain by-products of nylon 6 manufacture. It uses a set of enzymes to digest nylon, popularly known as nylonase.
Discovery and nomenclature
In 1975, a team of Japanese scientists discovered a strain of bacterium, living in ponds containing waste water from a nylon factory, that could digest certain byproducts of nylon 6 manufacture, such as the linear dimer of 6-aminohexanoate. These substances are not known to have existed before the invention of nylon in 1935. It was initially named as Achromobacter guttatus.
Studies in 1977 revealed that the three enzymes that the bacteria were using to digest the byproducts were significantly different from any other enzymes produced by any other bacteria, and not effective on any material other than the manmade nylon byproducts.
The bacterium is reassigned to Flavobacterium in 1980. Its genome was resolved in 2017, again reassigning it to Arthrobacter. The Genome Taxonomy Database considers it a strain of Paenarthrobacter ureafaciens following a 2016 reclassification. As of January 2021, the NCBI taxonomy browser has been updated to match GTDB.
A few newer strains have been created by growing the original KI72 in different conditions. These include KI722, KI723, KI723T1, KI725, KI725R, and many more.
The bacterium contains the following three enzymes:
- 6-aminohexanoate-cyclic-dimer hydrolase (EI, NylA, )
- 6-aminohexanoate-dimer hydrolase (EII, NylB, )
- 6-aminohexanoate-oligomer endohydrolase (EIII, NylC, )
EII has evolved by gene duplication followed by base substitution of another protein EII'. Both enzymes have 345 identical aminoacids out of 392 aminoacids (88% homology). The enzymes are similar to beta-lactamase.
The structure of EIII was resolved in 2018. Instead of being a completely novel enzyme, it appears to be a member of the N-terminal nucleophile (N-tn) hydrolase family. Specifically, computational approaches classifiy it as a MEROPS S58 (now renamed P1) hydrolase.
EIII was originally thought to be completely novel. Susumu Ohno proposed that it had come about from the combination of a gene-duplication event with a frameshift mutation. An insertion of thymidine change would turn an arginine-rich 427aa protein into the 392aa enzyme.
Role in evolution teaching
There is scientific consensus that the capacity to synthesize nylonase most probably developed as a single-step mutation that survived because it improved the fitness of the bacteria possessing the mutation. More importantly, the enzyme involved was produced by a mutation completely randomizing the original gene.[dubious ] Despite this, the new gene still had a novel, albeit weak, catalytic capacity. This is seen as a good example of how mutations easily can provide the raw material for evolution by natural selection.
A 1995 paper showed that scientists have also been able to induce another species of bacterium, Pseudomonas aeruginosa, to evolve the capability to break down the same nylon byproducts in a laboratory by forcing them to live in an environment with no other source of nutrients. The P. aeruginosa strain NK87 did not seem to use the same enzymes[dubious ] that had been utilized by the original KI72 strain.
- Organisms breaking down plastic
- Biodegradable plastic
- E. coli long-term evolution experiment
- Radiotrophic fungus
- London Underground mosquito
- Lonicera fly
- Mealworms are capable of digesting polystyrene
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