Aldobrandini was born 20 April 1623, the daughter of Giorgio Aldobrandini (Prince of Meldola, Sarsina and Rossano) and Ippolita Ludovisi (daughter of Orazio Ludovisi, sister of Niccolò Ludovisi and a niece of Pope Gregory XV).
In 1638, she married Prince Paolo Borghese of the Borghese family who died in 1646. The following year, in 1647, she married Camillo Pamphili (son of Olimpia Maidalchini and nephew of Pope Innocent X) who renounced a cardinalate to become her husband. Part of her dowry of her second marriage was a collection of paintings (including masterpieces removed from the Duke of Ferrara's "Camerino d’Alabastro"), villas in Montemagnanapoli and Frascati, the great Aldobrandini estates in Romagna on the Corso in Rome and the Palazzo Aldobrandini.
Aldobrandini and Camillo Pamphili had five children including Giovan Battista Pamphili, Benedetto Pamphili and Anna Pamphili who married the Genoese nobleman Giovanni Andrea III Doria Landi in 1671. When the Roman branch of the Pamphlili family ended in 1760, it was the descendants of Anna and Giovanni who inherited the palazzo in Rome.
Children with Prince Borghese:
- Giovanni Giorgio Borghese
- Camillo Borghese
- Francesco Borghese
- Giovanni Battista Borghese, Principe Borghese (1639-1717) married Eleonora Boncompagni and had issue (including Camillo Borghese, Prince of Sulmona, husband of Pauline Bonaparte).
- Maria Virginia Borghese (1642-1718) married Agostino Chigi, Prince of Farnese, Duke of Ariccia and had issue.
Children with Pamphili:
- Flaminia Pamphili (d.1709) married (1) Bernardino Savelli, Duke of Castelgandolfo and had no children; married (2) Niccolo Francesco Pallavicini, Prince of Civitella and had no children.
- Teresa Pamphili (1650-1704) married Carlo II Cybo-Malaspina, Duke of Massa and had progeny.
- Anna Pamphili (1652-1728) married Giannandrea Doria, Prince of Melfi and founded the Doria-Pamphili-Landi line.
- Giambattista Pamphili, Prince of Carpinetti & of Belvedere (d.1717) married Violante Facchinetti and a daughter Olimpia (1672-1731) who married Filippo Colonna, Prince of Paliano, son of Marie Mancini
- Pamphili family, with inclusive family tree.