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Classification and external resources
Specialty Infectious disease
ICD-10 B66.0
ICD-9-CM 121.0
DiseasesDB 29303
Patient UK Opisthorchiasis
MeSH D009889

Opisthorchiasis is a parasitic disease caused by species in the genus Opisthorchis (specifically, Opisthorchis viverrini and Opisthorchis felineus).

Medical care and loss of wages caused by Opisthorchis viverrini in Laos and in Thailand costs about $120 million annually[1] or $120 million per year can cost Northeast Thailand only.[2]

Infection of Opisthorchis viverrini and of other liver flukes in Asia affect the poor and poorest people.[3] Opisthorchiasis has received less attention than other diseases and is a neglected disease in Asia.[3]

Signs and symptoms[edit]

Symptoms of opisthorchiasis/clonorchiasis.
Main article: clonorchiasis

Symptoms of opisthorchiasis (caused by Opisthorchis spp.) are indistinguishable from clonorchiasis (caused by Clonorchis sinensis),[2] so the disease should be referred as clonorchiasis.[2]

About 80% of infected people have no symptoms, though they can have eosinophilia.[1] This is when the infection is weak and there are less than 1000 eggs in one gram in feces.[1]

When there are 10,000-30,000 eggs in one gram of feces, then the infection is heavy.[1] Symptoms of heavier infections with Opisthorchis viverrini may include: diarrhoea, pain in epigastric and pain in the upper right quadrant, lack of appetite (anorexia), fatigue, yellowing of the eyes and skin (jaundice) and mild fever.[1]

These parasites are long-lived and cause heavy chronic infections may led to accumulation of fluid in legs (edema) and in the peritoneal cavity (ascites),[1] enlarged non-functional gall-bladder[1] and also cholangitis, which can lead to periductal fibrosis, cholecystitis and cholelithiasis, obstructive jaundice, hepatomegaly and/or fibrosis of the periportal system.


For medical diagnosis there is need to find eggs of Opisthorchis viverrini in feces[1] using Kato technique.[4]

An antigen 89 kDa of Opisthorchis viverrini can be detected by ELISA test.[1]

A PCR test capable of amplifying a segment of the internal transcribed spacer region of ribosomal DNA for the opisthorchiid and heterophyid flukes eggs taken directly from faeces was developed and evaluated in a rural community in central Thailand.[5] The lowest quantity of DNA that could be amplified from individual adults of Opisthorchis viverrini was estimated to 0.6 pg.[5]


Currently, there is no effective chemotherapy to combat cholangiocarcinoma, such that intervention strategies need to rely on the prevention or treatment of liver fluke infection/disease. Although effective prevention could be readily achieved by persuading people to consume cooked fish (via education programs), the ancient cultural custom to consume raw, undercooked or freshly pickled fish persists in endemic areas. Cooking or deep-freezing (-20 °C for 7 days)[6] of food made of fish is sure method of prevention.[1] Methods for prevention of Opisthorchis viverrini in aquaculture fish ponds were proposed by Khamboonruang et al. (1997).[7]


There was unsuccessful use of chloroquine for opisthorchiasis treatment in 1951-1968.[4]

Thus, currently, the control of opisthorchiasis relies predominantly on antihelminthic treatment with praziquantel. The single dose of praziquantel of 40 mg/kg is effective against opisthorchiasis (and also against schistosomiasis).[4] Despite the efficacy of this compound, the lack of an acquired immunity to infection predisposes humans to reinfections in endemic regions. In addition, under experimental conditions, the short-term treatment of Opisthorchis viverrini-infected hamsters with praziquantel (400 mg per kg of live weight) has been shown to induce a dispersion of parasite antigens, resulting in adverse immunopathological changes as a result of oxidative and nitrative stresses following re-infection with Opisthorchis viverrini, a process which has been proposed to initiate and/or promote the development of cholangiocarcinoma in humans.[8] Albendazole can be used as an alternative.[9]

A randomised-controlled trial published in 2011 showed that the broad-spectrum anti-helminthic, tribendimidine, appears to be at least as efficacious as praziquantel.[10] Artemisinin was also found to have anthelmintic activity against Opisthorchis viverrini.[11]


Opisthorchiasis is prevalent where raw cyprinid fishes are a staple of the diet.[12] Prevalence rises with age; children under the age of 5 years are rarely infected by Opisthorchis viverrini. Males may be affected more than females.[13][14] The WHO estimates that foodborne trematodiases (infection by worms or "flukes", mainly Clonorchis, Opisthorchis, Fasciola and Paragonimus species) affect 56 million people worldwide and 750 million are at risk of infection.[15][16] Eighty million are at risk of opisthorchiasis,[17] 67 million from infection with Opisthorchis viverrini in Southeast Asia and 13 million from Opisthorchis felineus in Kazakhstan, Russia including Siberia, and Ukraine.[18] In the lower Mekong River basin, the disease is highly endemic, more so in lowlands,[12] with a prevalence up to 60% in some areas of northeast Thailand. However, estimates using newer polymerase chain reaction-based diagnostic techniques indicate that prevalence is probably grossly underestimated.[19] In one study from the 1980s, a prevalence of over 90% was found in persons greater than 10 years old in a small village near Khon Kaen in northeast Thailand in the region known as Isaan.[20] Sporadic cases have been reported in case reports from Malaysia, Singapore, and the Philippines.[16] Although overall prevalence declined after initial surveys in the 1950s, increases since the 1990s in some areas seem associated with large increases in aquaculture.[18]

Link to cholangiocarcinoma[edit]

Incidence of cholangiocarcinoma and O. viverrini in Thailand from 1990–2001.

Both experimental and epidemiological evidence strongly implicates Opisthorchis viverrini infections in the etiology of a malignant cancer of the bile ducts (cholangiocarcinoma) in humans which has a very poor prognosis.[21] Clonorchis sinensis and Opisthorchis viverrini are both categorized by the International Agency for Research on Cancer (IARC) as Group 1 carcinogens.

In humans, the onset of cholangiocarcinoma occurs with chronic opisthorchiasis, associated with hepatobiliary damage, inflammation, periductal fibrosis and/or cellular responses to antigens from the infecting fluke.[21] These conditions predispose to cholangiocarcinoma, possibly through an enhanced susceptibility of DNA to damage by carcinogens. Chronic hepatobiliary damage is reported to be multi-factorial and considered to arise from a continued mechanical irritation of the epithelium by the flukes present, particularly via their suckers, metabolites and excreted/secreted antigens as well as immunopathological processes. In silico analyses using techniques of genomics and bioinformatics is unraveling information on molecular mechanisms that may be relevant to the development of cholangiocarcinoma.[8]

In regions where Opisthorchis viverrini is highly endemic, the incidence of cholangiocarcinoma is unprecedented.[21] For instance, cholangiocarcinomas represent 15% of primary liver cancer worldwide, but in Thailand's Khon Kaen region, this figure escalates to 90%, the highest recorded incidence of this cancer in the world. Of all cancers worldwide from 2002, 0.02% were cholangiocarcinoma caused by Opisthorchis viverrini.[21] The cancer of the bile ducts caused by opisthorchiasis occur in the ages 25–44 years in Thailand.[4]


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