He was born and died in Bologna. A close friend of Lorenzo Sabbatini, Samacchini traveled to Rome where he participated in 1563 in the decoration of the Vatican Belvedere and of the Sala Regia of Pius IV, along with Taddeo Zuccari and his brother. He returned to Bologna, where he was influenced by Pellegrino Tibaldi. Sammacchini painted for the Palazzo Vitelli a Sant'Egidio, Città di Castello, San Giacomo Maggiore, the church of Corpus Domini in Bologna, and Santa Maria Maggiore (Santa Maria della Vita?) in Bologna. He painted frescoes of Virtues, Prophets and Angels in Sant'Abbondio, Cremona.
- Freedberg, Sydney J. (1993). Pelican History of Art (ed.). Painting in Italy, 1500-1600. Penguin Books. p. 567. ISBN 0-300-05587-0.
- Memorie originali italiane risguardanti le belle arti, Volume 1, by Michelangelo Gualandi, M.G. pages 25-29.
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