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Banai was born in Beersheba, Israel. Her father was a local judge, and her mother was an education executive for the City of Beersheba. Many of her relatives are successful Israeli actors and singers. The next generation of the Banai family, including Orna and her brothers, Meir and Eviatar, followed this tradition. After three years of studying acting in Nissan Nativ's studio in Tel Aviv, Banai started performing in stand-up comedy and entertainment shows.
Banai is most known for her character "Limor" in the humorous Israeli television shows Action and Rak BeIsrael (with Erez Tal). Since 2003, Banai has appeared in the popular satirical television show Eretz Nehederet. In addition, Banai acted in television dramas like Merchav-Yarkon and Max VeMoris. Banai also appeared in theatrical plays like Singles and The Last Striptease.
- Arad, Dafna (22 July 2014). "Israeli artists opposing the war come under attack on social networks". Haaretz. Retrieved 22 July 2014.