From Wikipedia, the free encyclopedia
Original author(s)David Emms
Steven Kelly
Developer(s)David Emms
Initial release2015
Written inPython
Operating systemLinux
LicenseGNU GPL v3
OrthoFinder workflow and outputs.

OrthoFinder is a command-line software tool for comparative genomics.[1][2] OrthoFinder determines the correspondence between genes in different organisms (also known as orthology analysis). This correspondence provides a framework for understanding the evolution of life on Earth, and enables the extrapolation and transfer of biological knowledge between organisms.

OrthoFinder takes FASTA files of protein sequences as input (one per species) and as output provides:

  • Orthogroups
  • Rooted Phylogenetic trees of all orthogroups
  • A rooted species tree for the set of species included in the input dataset
  • Hierarchical orthogroups for each node in the species tree
  • Orthologs between all species
  • Gene duplication events mapped to branches in the species tree
  • Comparative genomic statistics

As of August 2021, the tool has been referenced by more than 1500 published studies.[3]

See also[edit]


  1. ^ Emms, David M; Kelly Steven (2015). "OrthoFinder: solving fundamental biases in whole genome comparisons dramatically improves orthogroup inference accuracy". Genome Biology. 16 (157): 157. doi:10.1186/s13059-015-0721-2. PMC 4531804. PMID 26243257.
  2. ^ Emms, David M; Kelly Steven (2019). "OrthoFinder: phylogenetic orthology inference for comparative genomics". Genome Biology. 20 (238): 238. doi:10.1186/s13059-019-1832-y. PMC 6857279. PMID 31727128.
  3. ^ "Citations for Emms & Kelly 2015". Google Scholar. Retrieved 6 August 2021.