From Brno use road 43 (E461) direction Svitavy. Use exit Kuřim and Tišnov and follow the road 385 through Kuřim and Tišnov. In Tišnov there is a traffic circle -- continue straight (direction Předklášteří and Nové město na Moravě). Předklášteří is the first village right after exiting Tišnov (cca 2 km). After entering Předklášteří, turn left at the first crossroad to the village center. In the village you can find a local street signs, which will help you to locate the monastery.
From Brno take train line S3 and get off in Tišnov. There you can take a bus (lines 163, 334, 335 or 336) or you can use the blue Trail blaze and walk from the railway station to Předklašteří (cca 3 km).