P1-derived artificial chromosome

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A P1-derived artificial chromosome is a DNA construct that was derived from the DNA of P1 bacteriophage. It can carry large amounts (about 100–300 kilobases) of other sequences for a variety of bioengineering purposes. It is one type of vector used to clone DNA fragments (100- to 300-kb insert size; average, 150 kb) in Escherichia coli cells.

P1 was developed as a cloning vector by Nat Sternberg and colleagues in the 1990s.[1][2]

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  1. ^ Yarmolinsky M, Hoess R (November 2015). "The Legacy of Nat Sternberg: The Genesis of Cre-lox Technology". Annual Review of Virology. 2 (1): 25–40. doi:10.1146/annurev-virology-100114-054930. PMID 26958905.
  2. ^ Sternberg N (January 1990). "Bacteriophage P1 cloning system for the isolation, amplification, and recovery of DNA fragments as large as 100 kilobase pairs". Proceedings of the National Academy of Sciences of the United States of America. 87 (1): 103–7. Bibcode:1990PNAS...87..103S. doi:10.1073/pnas.87.1.103. JSTOR 2353636. PMC 53208. PMID 2404272.

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