This 85-kDa protein is one of two subunit types that comprise the seven tetrameric PFK isozymes. The muscle isozyme (PFK-1) is comprised solely of PFKM. The liver PFK (PFK-5) contains solely the second subunit type, PFKL, while the erythrocyte PFK includes five isozymes composed of different combinations of PFKM and PFKL. These subunits evolved from a common prokaryotic ancestor via gene duplication and mutation events. Generally, the [N-terminal]] of the subunits carries out their catalytic activity while the C-terminal contains allosteric ligand binding sites. In particular, the binding site for the PFK inhibitor citrate is found in the PFKL C-terminal region.
This gene encodes one of three protein subunits of PFK, which are expressed and combined to form the tetrameric PFK in a tissue-specific manner. As a PFK subunit, PFKL is involved in catalyzing the phosphorylation of fructose 6-phosphate to fructose 1,6-bisphosphate. This irreversible reaction serves as the major rate-limiting step of glycolysis.
Though the PFKM subunit majorly incorporates into muscle and erythrocyte PFKs, PFKM also is expressed in the heart, brain, and testis.
As the erythrocyte PFK comprises of both PFKL and PFKM, this heterogeneic composition is attributed with the differential PFK activity and organ involvement observed in some inherited PFK deficiency states in which myopathy or hemolysis or both can occur, such as glycogenosis type VII, also known as Tarui disease. Notably, mutations in PFKM have been shown to cause Tarui disease due to homozygosity for catalytically inactive M subunits. PFKM is confirmed to be involved in muscle PFK deficiency with early-onset hyperuricemia.
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