PNP agar

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PNP Agar is an agar medium used in microbiology to identify Staphylococcus species that have phosphatase activity. The medium changes color when p-nitrophenylphosphate disodium (PNP) is dephosphorylated.[1]

PNP agar is composed of Mueller-Hinton agar buffered to pH 5.6 to 5.8, with the addition of 0.495 mg/mL PNP.[2]


  1. ^ Janda, W.M.; K. Ristow; D. Novak (September 1994). "Evaluation of RapiDEC Staph for Identification of Staphylococcus aureus, Staphylococcus epidermidis, and Staphylococcus saprophyticus". Journal of Clinical Microbiology. 32 (9): 2056–2059. PMC 263941. PMID 7814525.
  2. ^ Geary, C.; M. Stevens (October 1989). "Detection of phosphatase production by Staphylococcus species: a new method". Med Lab Sci. 46 (4): 291–294. PMID 2559282.