POLI

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POLI
Protein POLI PDB 1t3n.png
Available structures
PDB Ortholog search: PDBe RCSB
Identifiers
Aliases POLI, RAD30B, RAD3OB, polymerase (DNA) iota, DNA polymerase iota
External IDs MGI: 1347081 HomoloGene: 5209 GeneCards: POLI
RNA expression pattern
PBB GE POLI 219317 at fs.png
More reference expression data
Orthologs
Species Human Mouse
Entrez
Ensembl
UniProt
RefSeq (mRNA)

NM_007195

NM_001136090
NM_001289515
NM_001289516
NM_011972

RefSeq (protein)

NP_001129562
NP_001276444
NP_001276445
NP_036102

Location (UCSC) Chr 18: 54.27 – 54.32 Mb Chr 18: 70.51 – 70.53 Mb
PubMed search [1] [2]
Wikidata
View/Edit Human View/Edit Mouse

DNA polymerase iota is an enzyme that in humans is encoded by the POLI gene.[3] It is found in higher eukaryotes, and is believed to have arisen from a gene duplication from Pol η. Pol ι, is a Y family polymerase that is involved in translesion synthesis. It can bypass 6-4 pyrimidine adducts and abasic sites and has a high frequency of wrong base incorporation. Like many other Y family polymerases Pol ι, has low processivity, a large DNA binding pocket and doesn't undergo conformational changes when DNA binds. These attributes are what allow Pol ι to carry out its task as a translesion polymerase. Pol ι only uses Hoogsteen base pairing, during DNA synthesis, it will add adenine opposite to thymine in the syn conformation and can add both cytosine and thymine in the anti conformation across guanine, which it flips to the syn conformation.


References[edit]

  1. ^ "Human PubMed Reference:". 
  2. ^ "Mouse PubMed Reference:". 
  3. ^ Frank EG, Woodgate R (Aug 2007). "Increased catalytic activity and altered fidelity of human DNA polymerase iota in the presence of manganese". J Biol Chem. 282 (34): 24689–96. PMID 17609217. doi:10.1074/jbc.M702159200. 

Further reading[edit]