POLQ

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POLQ
Available structures
PDBOrtholog search: PDBe RCSB
Identifiers
AliasesPOLQ, POLH, PRO0327, polymerase (DNA) theta, DNA polymerase theta
External IDsMGI: 2155399 HomoloGene: 32727 GeneCards: POLQ
Gene location (Human)
Chromosome 3 (human)
Chr.Chromosome 3 (human)[1]
Chromosome 3 (human)
Genomic location for POLQ
Genomic location for POLQ
Band3q13.33Start121,431,427 bp[1]
End121,546,641 bp[1]
RNA expression pattern
PBB GE POLQ 219510 at fs.png
More reference expression data
Orthologs
SpeciesHumanMouse
Entrez
Ensembl
UniProt
RefSeq (mRNA)

NM_199420

NM_001159369
NM_029977

RefSeq (protein)

NP_955452

NP_001152841
NP_084253

Location (UCSC)Chr 3: 121.43 – 121.55 MbChr 16: 37.01 – 37.1 Mb
PubMed search[3][4]
Wikidata
View/Edit HumanView/Edit Mouse

DNA polymerase theta is an enzyme that in humans is encoded by the POLQ gene.[5][6] Current evidence suggests that this polymerase is involved in repair of DNA double-strand breaks in a process termed microhomology-mediated end joining (MMEJ) .[7] Most double-strand breaks are repaired by non-homologous end joining (NHEJ) or homology directed repair (HDR). MMEJ is a variant of NHEJ, but differs in that it lacks a requirement for the Ku heterodimer, and involves DNA ends with 3′ single-stranded overhangs.[8] Following annealing of short (i.e., a few nucleotides) regions on the DNA overhangs, DNA polymerase theta catalyzes template-dependent DNA synthesis across the broken ends, stabilizing the paired structure.[9][10]

References[edit]

  1. ^ a b c GRCh38: Ensembl release 89: ENSG00000051341 - Ensembl, May 2017
  2. ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000034206 - Ensembl, May 2017
  3. ^ "Human PubMed Reference:".
  4. ^ "Mouse PubMed Reference:".
  5. ^ Sharief FS, Vojta PJ, Ropp PA, Copeland WC (Aug 1999). "Cloning and chromosomal mapping of the human DNA polymerase theta (POLQ), the eighth human DNA polymerase". Genomics. 59 (1): 90–6. doi:10.1006/geno.1999.5843. PMID 10395804.
  6. ^ "Entrez Gene: POLQ polymerase (DNA directed), theta".
  7. ^ Wood RD, Doublié S (August 2016). "DNA polymerase θ (POLQ), double-strand break repair, and cancer". DNA Repair (Amst). 44: 22–32. doi:10.1016/j.dnarep.2016.05.003. PMC 5114520. PMID 27264557.
  8. ^ Yousefzadeh MJ, Wyatt DW, Mu Y, Hensley SC, Tomida J, Bylund GO, Doublié S, Johansson E, Ramsden DA, McBride KM, Wood RD (October 2014). "Mechanism of suppression of chromosomal instability by DNA polymerase POLQ". PLoS Genet. 10 (10): e1004654. doi:10.1371/journal.pgen.1004654. PMID 25275444.
  9. ^ Mateos-Gomez PA, Gong F, Nair N, Miller KM, Lazzerini-Denchi E, Sfeir A (2015). "Mammalian polymerase θ promotes alternative NHEJ and suppresses recombination". Nature. 518 (7538): 254–7. doi:10.1038/nature14157. PMID 25642960.
  10. ^ Ceccaldi R, Liu JC, Amunugama R, Hajdu I, Primack B, Petalcorin MI, O'Connor KW, Konstantinopoulos PA, Elledge SJ, Boulton SJ, Yusufzai T, D'Andrea AD (2015). "Homologous-recombination-deficient tumours are dependent on Polθ-mediated repair". Nature. 518 (7538): 258–62. doi:10.1038/nature14184. PMID 25642963.

Further reading[edit]