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Protein PRPF8 PDB 3E9L.png
Available structures
PDB Ortholog search: PDBe RCSB
Aliases PRPF8, pre-mRNA processing factor 8, HPRP8, PRP8, PRPC8, RP13, SNRNP220
External IDs MGI: 2179381 HomoloGene: 4706 GeneCards: PRPF8
RNA expression pattern
PBB GE PRPF8 200004 at fs.png

PBB GE PRPF8 200001 at fs.png

PBB GE PRPF8 200003 s at fs.png
More reference expression data
Species Human Mouse
RefSeq (mRNA)



RefSeq (protein)



Location (UCSC) Chr 17: 1.65 – 1.68 Mb Chr 11: 75.49 – 75.51 Mb
PubMed search [1] [2]
View/Edit Human View/Edit Mouse

Pre-mRNA-processing-splicing factor 8 is a protein that in humans is encoded by the PRPF8 gene.[3][4]


Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this gene is a component of both U2- and U12-dependent spliceosomes, and found to be essential for the catalytic step II in pre-mRNA splicing process. It contains several WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to yeast Prp8 protein. This gene is a candidate gene for autosomal dominant retinitis pigmentosa.[5]


PRPF8 has been shown to interact with WDR57[6][7] and EFTUD2.[7]


  1. ^ "Human PubMed Reference:". 
  2. ^ "Mouse PubMed Reference:". 
  3. ^ McKie AB, McHale JC, Keen TJ, Tarttelin EE, Goliath R, van Lith-Verhoeven JJ, Greenberg J, Ramesar RS, Hoyng CB, Cremers FP, Mackey DA, Bhattacharya SS, Bird AC, Markham AF, Inglehearn CF (Jul 2001). "Mutations in the pre-mRNA splicing factor gene PRPC8 in autosomal dominant retinitis pigmentosa (RP13)". Human Molecular Genetics. 10 (15): 1555–62. PMID 11468273. doi:10.1093/hmg/10.15.1555. 
  4. ^ Luo HR, Moreau GA, Levin N, Moore MJ (Jul 1999). "The human Prp8 protein is a component of both U2- and U12-dependent spliceosomes". RNA. 5 (7): 893–908. PMC 1369814Freely accessible. PMID 10411133. doi:10.1017/S1355838299990520. 
  5. ^ "Entrez Gene: PRPF8 PRP8 pre-mRNA processing factor 8 homolog (S. cerevisiae)". 
  6. ^ Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S, McBroom-Cerajewski L, Robinson MD, O'Connor L, Li M, Taylor R, Dharsee M, Ho Y, Heilbut A, Moore L, Zhang S, Ornatsky O, Bukhman YV, Ethier M, Sheng Y, Vasilescu J, Abu-Farha M, Lambert JP, Duewel HS, Stewart II, Kuehl B, Hogue K, Colwill K, Gladwish K, Muskat B, Kinach R, Adams SL, Moran MF, Morin GB, Topaloglou T, Figeys D (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Molecular Systems Biology. 3 (1): 89. PMC 1847948Freely accessible. PMID 17353931. doi:10.1038/msb4100134. 
  7. ^ a b Achsel T, Ahrens K, Brahms H, Teigelkamp S, Lührmann R (Nov 1998). "The human U5-220kD protein (hPrp8) forms a stable RNA-free complex with several U5-specific proteins, including an RNA unwindase, a homologue of ribosomal elongation factor EF-2, and a novel WD-40 protein". Molecular and Cellular Biology. 18 (11): 6756–66. PMC 109259Freely accessible. PMID 9774689. doi:10.1128/mcb.18.11.6756. 

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