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Available structures
PDB Ortholog search: PDBe RCSB
Aliases PUF60, FIR, RoBPI, SIAHBP1, VRJS, poly(U) binding splicing factor 60KDa, poly(U) binding splicing factor 60
External IDs MGI: 1915209 HomoloGene: 8614 GeneCards: PUF60
RNA expression pattern
PBB GE SIAHBP1 209899 s at fs.png

PBB GE SIAHBP1 gnf1h08795 at fs.png
More reference expression data
Species Human Mouse
RefSeq (mRNA)


RefSeq (protein)
Location (UCSC) Chr 8: 143.82 – 143.83 Mb Chr 15: 76.07 – 76.08 Mb
PubMed search [1] [2]
View/Edit Human View/Edit Mouse

Poly(U)-binding-splicing factor PUF60 is a protein that in humans is encoded by the PUF60 gene.[3][4][5][6]

The protein encoded by this gene is a Ro RNP-binding protein. It interacts with Ro RNPs and their interaction is thought to represent a gain of function for Ro RNPs. This protein also forms a ternary complex with far upstream element (FUSE) and FUSE-binding protein. It can repress a c-myc reporter via the FUSE. It is also known to target transcription factor IIH and inhibit activated transcription. This gene is implicated in the xeroderma pigmentosum disorder. There are two alternatively spliced transcript variants of this gene encoding different isoforms. There seems to be evidence of multiple polyadenylation sites for this gene.[6]


PUF60 has been shown to interact with U2AF2.[7][8]


  1. ^ "Human PubMed Reference:". 
  2. ^ "Mouse PubMed Reference:". 
  3. ^ Bouffard P, Barbar E, Briere F, Boire G (Feb 2000). "Interaction cloning and characterization of RoBPI, a novel protein binding to human Ro ribonucleoproteins". RNA. 6 (1): 66–78. doi:10.1017/S1355838200990277. PMC 1369894Freely accessible. PMID 10668799. 
  4. ^ Liu J, He L, Collins I, Ge H, Libutti D, Li J, Egly JM, Levens D (Jul 2000). "The FBP interacting repressor targets TFIIH to inhibit activated transcription". Mol Cell. 5 (2): 331–41. doi:10.1016/S1097-2765(00)80428-1. PMID 10882074. 
  5. ^ Hastings ML, Allemand E, Duelli DM, Myers MP, Krainer AR (Jun 2007). Valcarcel J, ed. "Control of pre-mRNA splicing by the general splicing factors PUF60 and U2AF65". PLoS ONE. 2 (6): e538. doi:10.1371/journal.pone.0000538. PMC 1888729Freely accessible. PMID 17579712.  open access publication – free to read
  6. ^ a b "Entrez Gene: SIAHBP1 fuse-binding protein-interacting repressor". 
  7. ^ Rual, Jean-François; Venkatesan Kavitha; Hao Tong; Hirozane-Kishikawa Tomoko; Dricot Amélie; Li Ning; Berriz Gabriel F; Gibbons Francis D; Dreze Matija; Ayivi-Guedehoussou Nono; Klitgord Niels; Simon Christophe; Boxem Mike; Milstein Stuart; Rosenberg Jennifer; Goldberg Debra S; Zhang Lan V; Wong Sharyl L; Franklin Giovanni; Li Siming; Albala Joanna S; Lim Janghoo; Fraughton Carlene; Llamosas Estelle; Cevik Sebiha; Bex Camille; Lamesch Philippe; Sikorski Robert S; Vandenhaute Jean; Zoghbi Huda Y; Smolyar Alex; Bosak Stephanie; Sequerra Reynaldo; Doucette-Stamm Lynn; Cusick Michael E; Hill David E; Roth Frederick P; Vidal Marc (Oct 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. England. 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514. 
  8. ^ Ewing, Rob M; Chu Peter; Elisma Fred; Li Hongyan; Taylor Paul; Climie Shane; McBroom-Cerajewski Linda; Robinson Mark D; O'Connor Liam; Li Michael; Taylor Rod; Dharsee Moyez; Ho Yuen; Heilbut Adrian; Moore Lynda; Zhang Shudong; Ornatsky Olga; Bukhman Yury V; Ethier Martin; Sheng Yinglun; Vasilescu Julian; Abu-Farha Mohamed; Lambert Jean-Philippe; Duewel Henry S; Stewart Ian I; Kuehl Bonnie; Hogue Kelly; Colwill Karen; Gladwish Katharine; Muskat Brenda; Kinach Robert; Adams Sally-Lin; Moran Michael F; Morin Gregg B; Topaloglou Thodoros; Figeys Daniel (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Mol. Syst. Biol. England. 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948Freely accessible. PMID 17353931. 

Further reading[edit]