3D model (JSmol)
|Molar mass||2680.1386 grams/mol|
|Appearance||white amorphous hygroscopic solid|
|Melting point||decomposes at 300°C|
|Solubility||Very soluble in water, dimethyl sulfoxide, pyridine; slightly soluble in methanol and ethanol; insoluble in chloroform and diethyl ether|
|Main hazards||Highly toxic, symptoms of poisoning include: chest pains, breathing difficulties, tachycardia, unstable blood pressure and hemolysis.|
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
|what is ?)(|
Palytoxin, PTX or PLTX is an intense vasoconstrictor, and is considered to be one of the most poisonous non-protein substances known, second only to maitotoxin in terms of toxicity in mice.
Palytoxin is an polyhydroxylated and partially unsaturated compound (8 double bonds) with a long carbon chain. It has water- and fat-soluble parts, 40 hydroxy groups and 64 chiral centers. Due to chirality and possible double bond cis-trans isomerism, it has over 1021 alternative stereoisomers. It is thermostable, and treatment with boiling water does not remove its toxicity. It remains stable in aqueous solutions for prolonged periods, but rapidly decomposes and loses its toxicity in acidic or alkaline solutions. It has multiple analogues with a similar structure like ostreocin-D, mascarenotoxin-A and -B.
Palytoxin occurs at least in tropics and subtropics where it is made by Palythoa corals and Ostreopsis dinoflagellates, or possibly by bacteria occurring in these organisms. It can be found in many more species like fish and crabs due to the process of biomagnification. It can also be found in organisms living close to palytoxin producing organisms like sponges, mussels, starfish and cnidaria.
People are rarely exposed to palytoxin. Exposures have happened in people who have eaten sea animals like fish and crabs, but also in aquarium hobbyists who have handled Palythoa corals incorrectly and in those who have been exposed to certain algal blooms.
Palytoxin targets the sodium-potassium pump protein by locking it into a position where it allows passive transport of both sodium and potassium ions, thereby destroying the ion gradient that is essential for life. Because palytoxin can affect every type of cell in the body, the symptoms can be very different for the various routes of exposure.
Palytoxin's planar chemical structure was solved in 1981 by two research groups independently from each other. Stereochemistry was solved in 1982. Palytoxin carboxylic acid was synthesized by Yoshito Kishi and colleagues in 1989 and actual palytoxin in 1994 by Kishi and Suh.
According to an ancient Hawaiian legend, on the island of Maui near the harbor of Hana there was a village of fishermen haunted by a curse. Upon their return from the sea, one of the fishermen would go missing. One day, enraged by another loss, the fishermen assaulted a hunchbacked hermit deemed to be the culprit of the town's misery. While ripping the cloak off the hermit the villagers were shocked because they uncovered rows of sharp and triangular teeth within huge jaws. A shark god had been caught. It was clear that the missing villagers had been eaten by the god on their journeys to the sea. The men mercilessly tore the shark god into pieces, burned him and threw the ashes into a tide pool near the harbor of Hana. Shortly after, a thick brown "moss" started to grow on the walls of the tide pool causing instant death to victims hit by spears smeared with the moss. Thus was the evil of the demon. The moss growing in the cursed tide pool became known as "limu-make-o-Hana" which literally means "seaweed of death from Hana." The Hawaiians believed that an ill curse came over them if they tried to collect the deadly "seaweed".
Palytoxin was first isolated, named and described from Palythoa toxica by Moore and Scheuer in a study published in 1971. They measured that its molar mass is approximately 3300 g/mol. They also identified it to be the substance that was probably responsible for the toxicity of P. toxica, but it was uncertain at the time if the coral also had other toxic compounds in it. It was then assessed by Walsh and Bowers that the limu-make-o-Hana was not a seaweed but a zoanthid coral, subsequently described as Palythoa toxica. Moore and Scheuer were aware of the study that Walsh and Bowers were writing.
Structure and total synthesis
In 1978 by plasmadesorption the mass of the palytoxin was measured to be 2861 g/mol and that it had 8 double bonds. Because palytoxin is such a large molecule, it took some time before the complete structure (including stereochemistry) was elucidated. Uemura et al. solved its planar chemical structure first and published their results in January 1981. Shortly afterwards Moore and Bartolini solved the same structure and published their results in May 1981. Forementioned groups solved the structure independently from each other. Palytoxin's stereochemistry was solved first by Moore et al in June 1982 and then by Uemura et al. in December in a study of four parts.
Palytoxin carboxylic acid was synthesized in 1989 by the group of Harvard professor Yoshito Kishi. Synthesis happened in 8 parts and then the parts were joined together to form the carboxylic acid. In 1994 Kishi et al. succeeded in making the actual palytoxin from this carboxylic acid. The accomplishment of palytoxin carboxylic acid synthesis was described as "the Mount Everest of organic synthesis, the largest single molecule that anyone has ever even thought about making" by Crawford in 1989.
Some of the organisms that contain palytoxin or its close analogues are listed below. These are either able to produce these compounds or have been found to contain them in some occasions due to bioaccumulation.
Certain bacteria might be able to produce palytoxin and may be the actual producers in some of the organisms listed above. Bacteria that have some evidence of palytoxin or its analogue production include Pseudomonas, Brevibacterium, Acinetobacter, Bacillus cereus, Vibrio sp. ja Aeromonas.
The toxicity of palytoxin is due to its binding to external part of Na+/K+-ATPase (the sodium–potassium pump), where it interacts with the natural binding site of ouabain with very high affinity. Na+/K+-ATPase is a transmembrane protein, which is found on the surface of every vertebrate cell. The sodium–potassium pump is necessary for viability of all cells, and this explains the fact that palytoxin affects all cells. Through this channel, which it forms within the sodium–potassium pump, monovalent positive ions such as sodium and potassium can diffuse freely, thereby destroying the ion gradient of the cell. Once palytoxin is bound to the pump, it flips constantly between open and normal conformations. The open conformation is more likely (over 90% probability). If palytoxin detaches, the pump will return to closed conformation. In open conformation, millions of ions diffuse through the pump per second, whereas only about one hundred ions per second are transported through a normally functioning transporter.
First evidence of the mechanism described above was obtained in 1981 and the proposed mechanism was published in 1982. Because the mechanism of action of palytoxin was so unlike any other, it was initially not widely accepted. This was primarily because it was not expected that a pump which provides active transport, could become an ion channel by binding of a compound such as palytoxin. Therefore, there were some alternative hypotheses, which were reviewed by Frelin and van Renterghem in 1995. The breakthrough research which is seen as proof for the sodium–potassium pump mechanism was performed in yeast cells (Saccharomyces cerevisiae). These cells do not have the sodium–potassium pump, and hence palytoxin does not affect them. But once they were given the DNA to encode for complete sheep Na+/K+-ATPase, they were killed by palytoxin.
From intravenous (IV) animal studies the toxic dose (LD50) of palytoxin via IV for humans has been estimated by extrapolation to be between 2.3–31.5 micrograms (µg) of palytoxin. An acute oral reference dose has been suggested to be 64 µg for a person with weight of 60 kg. Acute reference dose means a dose that can be safely ingested over a short period of time, usually during one meal or one day.
In comparison to IV injection, the toxicity of palytoxin in various animals via intramuscular and subcutaneous injections are 2.5 and 4–30 times higher, respectively. Upon ingestion the toxicity in animals has been 200 times less than via IV. In the table below, there are listed some LD50 values for partially pure palytoxin obtained from different Palythoa. These values represent the amount of palytoxin required to kill half of the test animals. Values are in micrograms (µg) per kilogram of the animal's weight and have been measured 24 hours after the initial exposure.
|Oral||Mouse||510 or 767|
An early toxicological characterization classified palytoxin as "relatively non-toxic" after intragastric administration to rats. The lethal dose (LD50) was greater than 40 µg/kg. The LD50 after parenteral administration was lower than 1 µg/kg. However the doubtful purity of this study increased because of uncertainty concerning the toxicological data. In 1974, the structure of palytoxin was not completely elucidated and the molecular weight was a lot higher (3300 Da instead of 2681 Da). A 2004 study discovered an LD50 of 510 µg/kg after intragastric administration in mice, but histological or biochemical information was missing. (Rhodes and Munday, 2004) Furthermore, palytoxin was not lethal to mice given an oral dose of 200 µg/kg. It was also found that palytoxin is very toxic after intraperitoneal injection. The LD50 in mice was less than 1 µg/kg. Because toxin-producing organisms spread to temperate climates and palytoxin-contaminated shellfish were discovered in the Mediterranean Sea a study was done to better define the toxic effects of palytoxin after oral exposure in mice. Palytoxin was lethal from 600 µg/kg doses. The number of deaths were dose-dependent and the LD50 calculated to be 767 µg/kg. This is comparable to the LD50 of 510 µg/kg referred by Munday (2008). The toxicity was not different if the mice had some food in their stomach. The oral toxicity is several times lower than the intraperitoneal toxicity. One of the possible causes of this behavior is that palytoxin is a very big hydrophilic molecule and therefore the absorption could be less efficient through the gastrointestinal tract than through the peritoneum. A recent study by Fernandez et al. further investigated on this issue using an in vitro model of intestinal permeability with differentiated monolayers of human colonic Caco-2 cells, confirming that palytoxin was unable to cross the intestinal barrier significantly, despite the damage the toxin exerted on cells and on the integrity of the monolayer. The same study also revealed that palytoxin does not affect tight-junctions on such cells. Palytoxin is most toxic after intravenous injection. The LD50 in mice is 0.045 µg/kg and in rats 0.089 µg/kg. In other mammals (rabbits, dogs, monkeys and guinea pigs) the LD50 is ranged between 0.025 and 0.45 µg/kg. They all died in several minutes from heart failure. The lethal dose for mice by the intratracheal route is above 2 µg/kg in 2 hours. Palytoxin is also very toxic after intramuscular or subcutaneous injection. No toxicity is found after intrarectal administration. Palytoxin is not lethal when topically applied to skin or eyes. Palytoxin can travel in water vapor and cause poisoning by inhalation.
In this context despite the increasing reports of palytoxin contaminated seafood in temperate waters (i.e., Mediterranean Sea), there are no validated and accepted protocols for the detection and quantification of this class of biomolecules. However, in the last years, many methodologies has been described with particular attention on the develop of new techniques for the ultrasensitive detection of palytoxin in real matrix such as mussels and microalgae (based on LC-MS-MS or immunoassay).
The symptoms of palytoxin poisoning and how quickly they appear depend partially on how much and through what route one has been exposed, e.g. if the poison has been inhaled or if the exposure has happened via skin.
In some non-lethal cases the symptoms in people have appeared in 6–8 hours after inhalation or skin exposure, and have lasted for 1–2 days. In different animals the symptoms have appeared in 30–60 minutes after intravenous injection and after 4 hours of eye-exposure.
The most common complication of severe palytoxin poisoning is rhabdomyolysis. This involves skeletal muscle breakdown and the leakage of intracellular contents into the blood. Other symptoms in humans are bitter/metallic taste, abdominal cramps, nausea, vomiting, diarrhea, mild to acute lethargy, tingling, slow heart rate, kidney failure, impairment of sensation, muscle spasms, tremor myalgia, cyanosis, and respiratory distress. In lethal cases palytoxin usually causes death by cardiac arrest via myocardial injury.
Exposure to aerosols of palytoxin analogue ovatoxin-a have resulted mainly in respiratory illness. Other symptoms caused by these aerosols included fever associated with serious respiratory disturbances, such as bronchoconstriction, mild dyspnea, and wheezes, while conjunctivitis was observed in some cases.
Clupeotoxism, poisoning after consuming clupeoid fish, is also suggested to be caused by palytoxin. Neurological and gastrointestinal disturbances are associated with clupeotoxism. Haff disease might be related to palytoxin and is characterized by rhabdomyolysis and gastrointestinal problems. In addition to ciguatoxin, palytoxin could be related to ciguatera seafood poisoning in some cases and thus give rise to a number of symptoms in this poisoning.
Animal studies have shown that vasodilators, such as papaverine and isosorbide dinitrate, can be used as antidotes. The animal experiments only showed benefit if the antidotes were injected into the heart immediately following exposure.
There have been cases where people died after eating foods containing palytoxin or poisons similar to it. In the Philippines people died after eating Demania reynaudii, a crab species. After eating bluestripe herring some people died in Madagascar. People who had eaten smoked fish and parrotfish experienced near fatal poisoning in Hawaii and Japan respectively.
Cases of inhalation are also known. A man inhaled palytoxin when he tried to kill a Palythoa in his aquarium with boiling water. In 2018, six people from Steventon, Oxfordshire, England were hospitalized after probable exposure by inhalation to "palytoxins" which were released by coral that was being removed from a personal aquarium. Four firefighters, who responded to the incident, were also hospitalized. The patients presented "flu-like symptoms" and eye-irritation. Also in 2018, a woman in Cedar Park, Texas was poisoned when she scraped growing algae from Palythoa polyps in her home aquarium. Other members of the family, including children, also reportedly fell ill. The woman described intense flu-like respiratory symptoms and high fever within hours of inhalation and was hospitalized. Confused physicians initially misdiagnosed the palytoxin poisoning to viral infection. The toxin also killed most of the fish in the aquarium. Many aquatic hobbyists purchase the coral for their bright coloring unaware of the toxins present and the danger of the toxin if it is disturbed.
A formerly unknown derivative of palytoxin, ovatoxin-a, produced as a marine aerosol by the tropical dinoflagellate Ostreopsis ovata caused hundreds of people in Genoa, Italy, to fall ill. In 2005 and 2006 blooms of these algae occurred in the Mediterranean sea. All those affected needed hospitalization. Symptoms were high fever, coughs and wheezes.
- Budavari, Susan, ed. (2001), The Merck Index: An Encyclopedia of Chemicals, Drugs, and Biologicals (13th ed.), Merck, ISBN 0911910131
- Deeds JR, Schwartz MD (August 2010). "Human risk associated with palytoxin exposure". Toxicon. 56 (2): 150–62. doi:10.1016/j.toxicon.2009.05.035. PMID 19505494.
- Ramos V, Vasconcelos V (June 2010). "Palytoxin and analogs: biological and ecological effects". Marine Drugs. 8 (7): 2021–37. doi:10.3390/md8072021. PMC 2920541. PMID 20714422.
- Pelin M, Brovedani V, Sosa S, Tubaro A (February 2016). "Palytoxin-Containing Aquarium Soft Corals as an Emerging Sanitary Problem". Marine Drugs. 14 (2): 33. doi:10.3390/md14020033. PMC 4771986. PMID 26861356.
- Sud P, Su MK, Greller HA, Majlesi N, Gupta A (September 2013). "Case series: inhaled coral vapor--toxicity in a tank". Journal of Medical Toxicology. 9 (3): 282–6. doi:10.1007/s13181-013-0307-x. PMC 3770997. PMID 23702624.
- Gadsby DC, Takeuchi A, Artigas P, Reyes N (January 2009). "Review. Peering into an ATPase ion pump with single-channel recordings". Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences. 364 (1514): 229–38. doi:10.1098/rstb.2008.0243. PMC 2674102. PMID 18986966.
- Moore RE, Bartolini G, et al. (June 1982). "Absolute Stereochemistry of Palytoxin". Journal of the American Chemical Society. 104 (13): 3776–3779. doi:10.1021/ja00377a064.
- Klein LL, McWhorter WW, Ko SS, Pfaff KP, KishiB Y, Uemura D, Hirata Y (December 1982). "Stereochemistry of palytoxin. Part 1. C85-C115 segment". Journal of the American Chemical Society. 104 (25): 7362–7364. doi:10.1021/ja00389a098.
- Cha JK, Christ WJ, Finan JM, Fujioka H, Kishi Y, Klein LL, et al. (December 1982). "Stereochemistry of palytoxin. Part 4. Complete structure". Journal of the American Chemical Society. 104 (25): 7369–7371. doi:10.1021/ja00389a101. ISSN 0002-7863.
- Armstrong RW, Beau JM, Cheon SH, Christ WJ, Fujioka H, Ham WH, et al. (1989). "Total Synthesis of Palytoxin Carboxylic Acid and Palytoxin Amide". J. Am. Chem. Soc. 111 (19): 7530. doi:10.1021/ja00201a038.
- Suh EM, Kishi Y (1994). "Synthesis of Palytoxin from Palytoxin Carboxylic Acid". J. Am. Chem. Soc. 116 (24): 11205. doi:10.1021/ja00103a065.
- Malo D (1 July 1987) . Moolelo Hawaii [Hawaiian Antiquities] (in Hawaiian). Translated by Nathaniel Bright Emerson (2nd ed.). Bishop Museum Press. p. 201. ISBN 0-910240-15-9.
- Ciminiello P, Dell'Aversano C, Fattorusso E, Forino M (2010). "Palytoxins: A still haunting Hawaiian curse". Phytochemistry Reviews. 9 (4): 491–500. doi:10.1007/s11101-010-9185-x.
- Moore RE, Scheuer PJ (April 1971). "Palytoxin: a new marine toxin from a coelenterate". Science. 172 (3982): 495–8. doi:10.1126/science.172.3982.495. PMID 4396320.
- Walsh GE, Bowers RL (May 1971). "A review of Hawaiian zoanthids with descriptions of three new species". Zoological Journal of the Linnean Society. 50 (2): 161–180. doi:10.1111/j.1096-3642.1971.tb00757.x.
- Macfarlane RD, Uemura D, Ueda K, Hirata Y (January 1980). "Californium-252 plasma desorption mass spectrometry of palytoxin". Journal of the American Chemical Society. 102 (2): 875–876. doi:10.1021/ja00522a088.
- "CSJ Award 2005-Prof. Daisuke Uemura". www.csj.jp. Chemical Society of Japan, et al. Archived from the original on 28 March 2018. Retrieved 26 April 2018.
Its structural determination presented many difficulties. Dr. Uemura elucidated its planar structure in 1981 by repeatedly carrying out site-specific oxidative degradation and determined the structure of the degraded products using a sample that was originally isolated from Palythoa tuberculosa of Okinawa[n] origin.
- Uemura D, Ueda K, Hirata Y, Naoki H, Iwashita T (January 1981). "Further studies on palytoxin. I.". Tetrahedron Letters. 22 (20): 1909–1912. doi:10.1016/s0040-4039(01)90475-7.
- Uemura D, Ueda K, Hirata Y, Naoki H, Iwashita T (January 1981). "Further studies on palytoxin. II". Tetrahedron Letters. 22 (20): 2781–2784. doi:10.1016/S0040-4039(01)90551-9.
- Moore RE, Bartolini G (May 1981). "Structure of palytoxin". Journal of the American Chemical Society. 103 (9): 2491–2494. doi:10.1021/ja00399a093.
- Crawford MH (October 1989). "Harvard synthesizes palytoxin molecule". Science. 246 (4926): 34. doi:10.1126/science.246.4926.34-c. PMID 17837760.
- Wu CH (December 2009). "Palytoxin: membrane mechanisms of action". Toxicon. 54 (8): 1183–9. doi:10.1016/j.toxicon.2009.02.030. PMID 19269304.
- Habermann E (1989). "Palytoxin acts through Na+,K+-ATPase". Toxicon. 27 (11): 1171–87. doi:10.1016/0041-0101(89)90026-3. PMID 2575806.
- Redondo J, Fiedler B, Scheiner-Bobis G (January 1996). "Palytoxin-induced Na+ influx into yeast cells expressing the mammalian sodium pump is due to the formation of a channel within the enzyme". Molecular Pharmacology. 49 (1): 49–57. PMID 8569711.
- Habermann E, Chhatwal GS (1 May 1982). "Ouabain inhibits the increase due to palytoxin of cation permeability of erythrocytes". Naunyn-Schmiedeberg's Archives of Pharmacology. 319 (2): 101–107. doi:10.1007/BF00503920.
- Frelin C, Van Renterghem C (January 1995). "Palytoxin. Recent electrophysiological and pharmacological evidence for several mechanisms of action". General Pharmacology. 26 (1): 33–7. doi:10.1016/0306-3623(94)00133-8. PMID 7713364.
- Scheiner-Bobis G, Meyer zu Heringdorf D, Christ M, Habermann E (June 1994). "Palytoxin induces K+ efflux from yeast cells expressing the mammalian sodium pump". Molecular Pharmacology. 45 (6): 1132–6. PMID 7912814.
- Riobó, P; Paz, B; Franco, JM (2006). "Analysis of palytoxin-like in Ostreopsis cultures by liquid chromatography with precolumn derivatization and fluorescence detection". Analytica Chimica Acta. 566 (2): 217–223. doi:10.1016/j.aca.2006.03.013. ISSN 0003-2670.
- "Guidance for the Setting of an Acute Reference Dose (ARfD)" (PDF). European Commission. 5 July 2001. Archived (PDF) from the original on 30 April 2018.
- Wiles JS, Vick JA, Christensen MK (August 1974). "Toxicological evaluation of palytoxin in several animal species". Toxicon. 12 (4): 427–33. doi:10.1016/0041-0101(74)90011-7. PMID 4155146.
- Ito E, Yasumoto T (September 2009). "Toxicological studies on palytoxin and ostreocin-D administered to mice by three different routes". Toxicon. 54 (3): 244–51. doi:10.1016/j.toxicon.2009.04.009. PMID 19376151.
- Rhodes LL, Towers N, Briggs L, Munday R, Adamson JE (2002). "Uptake of palytoxin like compounds by shellfish fed Ostreopsis siamensis (Dinophyceae)". New Zealand J. Med. Freshwater Res. 36 (3): 631–636. doi:10.1080/00288330.2002.9517118.
- Aligizaki K, Katikou P, Nikolaidis G, Panou A (March 2008). "First episode of shellfish contamination by palytoxin-like compounds from Ostreopsis species (Aegean Sea, Greece)". Toxicon. 51 (3): 418–27. doi:10.1016/j.toxicon.2007.10.016. PMID 18067938.
- Ito E, Ohkusu M, Yasumoto T (June 1996). "Intestinal injuries caused by experimental palytoxicosis in mice". Toxicon. 34 (6): 643–52. doi:10.1016/0041-0101(96)00005-0. PMID 8817810.
- Fernández DA, Louzao MC, Vilariño N, Espiña B, Fraga M, Vieytes MR, Román A, Poli M, Botana LM (August 2013). "The kinetic, mechanistic and cytomorphological effects of palytoxin in human intestinal cells (Caco-2) explain its lower-than-parenteral oral toxicity". The FEBS Journal. 280 (16): 3906–19. doi:10.1111/febs.12390. PMID 23773601.
- Ciminiello P, Dell'Aversano C, Dello Iacovo E, Fattorusso E, Forino M, Tartaglione L (March 2011). "LC-MS of palytoxin and its analogues: State of the art and future perspectives". Toxicon. 57 (3): 376–89. doi:10.1016/j.toxicon.2010.11.002. PMID 21070802.
- Zamolo VA, Valenti G, Venturelli E, Chaloin O, Marcaccio M, Boscolo S, Castagnola V, Sosa S, Berti F, Fontanive G, Poli M, Tubaro A, Bianco A, Paolucci F, Prato M (September 2012). "Highly sensitive electrochemiluminescent nanobiosensor for the detection of palytoxin". ACS Nano. 6 (9): 7989–97. doi:10.1021/nn302573c. PMID 22913785.
- Louzao MC, Ares IR, Cagide E (December 2008). "Marine toxins and the cytoskeleton: a new view of palytoxin toxicity". The FEBS Journal. 275 (24): 6067–74. doi:10.1111/j.1742-4658.2008.06712.x. PMID 19016862.
- Thakur LK, Jha KK (21 October 2016). "Palytoxin-induced acute respiratory failure". Respiratory Medicine Case Reports. 20: 4–6. doi:10.1016/j.rmcr.2016.10.014. PMC 5099280. PMID 27843763.
- Alcala AC, Alcala LC, Garth JS, Yasumura D, Yasumoto T (1988). "Human fatality due to ingestion of the crab Demania reynaudii that contained a palytoxin-like toxin". Toxicon. 26 (1): 105–7. doi:10.1016/0041-0101(88)90142-0. PMID 2894726.
- Onuma Y, Satake M, Ukena T, Roux J, Chanteau S, Rasolofonirina N, Ratsimaloto M, Naoki H, Yasumoto T (January 1999). "Identification of putative palytoxin as the cause of clupeotoxism". Toxicon. 37 (1): 55–65. doi:10.1016/S0041-0101(98)00133-0. PMID 9920480.
- Kodama AM, Hokama Y, Yasumoto T, Fukui M, Manea SJ, Sutherland N (1989). "Clinical and laboratory findings implicating palytoxin as cause of ciguatera poisoning due to Decapterus macrosoma (mackerel)". Toxicon. 27 (9): 1051–3. doi:10.1016/0041-0101(89)90156-6. PMID 2572075.
- Okano H, Masuoka H, Kamei S, Seko T, Koyabu S, Tsuneoka K, Tamai T, Ueda K, Nakazawa S, Sugawa M, Suzuki H, Watanabe M, Yatani R, Nakano T (1998). "Rhabdomyolysis and myocardial damage induced by palytoxin, a toxin of blue humphead parrotfish". Intern. Med. 37 (3): 330–333. doi:10.2169/internalmedicine.37.330.
- Hoffmann K, Hermanns-Clausen M, Buhl C, Büchler MW, Schemmer P, Mebs D, Kauferstein S (June 2008). "A case of palytoxin poisoning due to contact with zoanthid corals through a skin injury". Toxicon. 51 (8): 1535–7. doi:10.1016/j.toxicon.2008.03.009. PMID 18433818.
- Majlesi N, Su MK, Chan GM, Lee DC, Greller HA (2008). "A case of inhalational exposure to palytoxin". Clin. Toxicol. 46: 637.
- "Fish tank fumes leave 10 in hospital". BBC News. 27 March 2018. Archived from the original on 15 April 2018. Retrieved 27 April 2018.
- Perez P (27 April 2018). "Cedar Park mother warning others after aquarium coral nearly kills her family". KENS. Archived from the original on 29 April 2018. Retrieved 29 April 2018.