para-Nitrophenylphosphate

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para-Nitrophenylphosphate
Skeletal formula of para-nitrophenylphosphate
Space-filling model of the para-nitrophenylphosphate molecule
Names
IUPAC name
(4-nitrophenyl) dihydrogen phosphate
Other names
pNPP
Identifiers
330-13-2 N
ChEBI CHEBI:17440 N
ChEMBL ChEMBL24231 N
ChemSpider 369 N
Jmol 3D model Interactive image
PubChem 378
Properties
C6H6NO6P
Molar mass 219.088701
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
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Infobox references

para-Nitrophenylphosphate (pNPP) is a chromogenic substrate for acid and alkaline phosphatase in ELISA and conventional spectrophotometric assays. These enzymes catalyse the hydrolysis of pNPP to yellow para-nitrophenol. This product absorbs strongly at 405 nm and can be measured with a visible light spectrophotometer.

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