para-Nitrophenylphosphate

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para-Nitrophenylphosphate
Skeletal formula of para-nitrophenylphosphate
Space-filling model of the para-nitrophenylphosphate molecule
Names
IUPAC name
(4-nitrophenyl) dihydrogen phosphate
Other names
pNPP
Identifiers
3D model (JSmol)
ChEBI
ChemSpider
ECHA InfoCard 100.005.777
Properties
C6H6NO6P
Molar mass 317.14
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
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Infobox references

para-Nitrophenylphosphate (pNPP) is a non-proteinaceous chromogenic substrate for alkaline and acid phosphatases used in ELISA and conventional spectrophotometric assays.[1] Phosphatases catalyze the hydrolysis of pNPP liberating inorganic phosphate and the conjugate base of para-nitrophenol (pNP). The resulting phenolate is yellow, with a maximal absorption at 405 nm.[2] This property can be used to determine the activity of various phosphatases including alkaline phosphatase (AP) and protein tyrosine phosphatase (PTP). The substance is sensitive to light, and thus should be stored protected from light. This is also important after adding the substrate to the mixture and before reading. -20°C is the optimal storage temperature.

Refhttps://www.neb.com/products/p0757-p-nitrophenyl-phosphate-pnpperences[edit]

  1. ^ Lorenz, Ulrike (2017-05-07). "Protein Tyrosine Phosphatase Assays". Current protocols in immunology / edited by John E. Coligan ... [et al.] CHAPTER: Unit–11.7. ISSN 1934-3671. PMC 3097125Freely accessible. PMID 21462163. doi:10.1002/0471142735.im1107s93. 
  2. ^ MacKintosh, C. (1993). In D.G. Hardie (Ed.). Protein Phosphorylation: A Practical Approach. 221. New York: IRL Press.

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