Pembrolizumab

From Wikipedia, the free encyclopedia
Jump to: navigation, search
Pembrolizumab
Pembrolizumab 5DK3.png
From PDB entry 5dk3
Monoclonal antibody
Type Whole antibody
Source Humanized (from mouse)
Target PD-1
Clinical data
Trade names Keytruda
Pregnancy
category
  • not for pregnant women
Routes of
administration
IV
ATC code
Legal status
Legal status
Identifiers
CAS Number
PubChem SID
DrugBank
ChemSpider
  • none
UNII
KEGG
Chemical and physical data
Formula C6534H10004N1716O2036S46
Molar mass 146 kDa

Pembrolizumab (formerly MK-3475 and lambrolizumab, trade name Keytruda)[1] is a humanized antibody used in cancer immunotherapy. It blocks a protective mechanism on cancer cells, and allows the immune system to destroy those cancer cells. It targets the programmed cell death 1 (PD-1) receptor. The drug was initially used to treat metastatic melanoma. In May 2017 it was approved for any unresectable or metastatic solid tumor with certain genetic qualities without regard to the tissue type or site of the tumor, a first for the FDA.

Medical uses[edit]

As of 2017, pembrolizumab is used via intravenous infusion to treat inoperable or metastatic melanoma, metastatic non-small cell lung cancer (NSCLC) in certain situations, as a second-line treatment for head and neck squamous cell carcinoma (HNSCC), after platinum-based chemotherapy, and for the treatment of adult and pediatric patients with refractory classic Hodgkin's lymphoma (cHL).[2][3][4][5][6]

For NSCLC, pembrolizumab is a first line treatment if the cancer overexpresses PD-L1, a PD-1 receptor ligand, and the cancer has no mutations in EGFR or in ALK; if chemotherapy has already been administered, then pembrolizumab can be used as a second line treatment but if the cancer has EGFR or ALK mutations, agents targeting those mutations should be used first.[2][7] Assessment of PD-L1 must be conducted with a validated and approved companion diagnostic.[2]

Contraindications[edit]

If a person is taking corticosteroids or immunosuppressants those drugs should be stopped before starting pembrolizumab because they may interfere with pembrolizumab; they can be used after pembrolizumab is started to deal with immune-related adverse effects.[3]

Women of child-bearing age should use contraception when taking pembrolizumab; it should not be administered to pregnant women because animal studies have shown that it can reduce tolerance to the fetus and increases the risk of miscarriage. It is not known if pembrolizumab is secreted into breast milk or not.[3]

As of 2017, the drug had not been tested in people with active infections including any HIV, hepatitis B or hepatitis C infection, kidney or liver disease, active CNS metastases, active systemic autoimmune disease, interstitial lung disease; prior pneumonia, and people with a history of severe reaction to another monoclonal antibody.[3]

Adverse effects[edit]

People have had severe infusion-related reactions to pembrolizumab. There have also been severe immune-related adverse effects including lung inflammation (including fatal cases) and inflammation of endocrine organs that caused inflammation of the pituitary gland, of the thyroid (causing both hypothyroidism and hyperthyroidism in different people), and pancreatitis that caused Type 1 diabetes and diabetic ketoacidosis; some people have had to go on lifelong hormone therapy as a result (e.g. insulin therapy or thyroid hormones). People have also had colon inflammation, liver inflammation, kidney inflammation due to the drug.[3][8]

The common adverse reactions have been fatigue (24%), rash (19%), itchiness (pruritus) (17%), diarrhea (12%), nausea (11%) and joint pain (arthralgia) (10%).[3]

Other adverse effects occurring in between 1% and 10% of people taking pembrolizumab have included anemia, decreased appetite, headache, dizziness, distortion of the sense of taste, dry eye, high blood pressure, abdominal pain, constipation, dry mouth, severe skin reactions, vitiligo, various kinds of acne, dry skin, eczema, muscle pain, pain in a limb, arthritis, weakness, edema, fever, chills, and flu-like symptoms.[3]

Mechanism of action[edit]

Pembrolizumab is a therapeutic antibody that binds to and blocks the PD-1, programmed cell death protein 1 located on lymphocytes. This receptor is generally responsible for preventing the immune system from attacking the body's own tissues; it is a so-called immune checkpoint.[9][10] Many cancers make proteins that bind to PD-1, thus shutting down the ability of the body to kill the cancer on its own.[9] Inhibiting PD-1 on the lymphocytes prevents this, allowing the immune system to target and destroy cancer cells;[11] this same mechanism also allows the immune system to attack the body itself, and checkpoint inhibitors like pembrolizumab have immune-dysfunction side effects as a result.[10]

Tumors that have mutations that cause DNA mismatch repair, which often results in microsatellite instability, tend to generate many mutated proteins that could serve as tumor antigens; pembrolizumab appears to facilitate clearance of any such tumor by the immune system, by preventing the self-checkpoint system from blocking the clearance.[12]

Pharmacology[edit]

Since pembrolizumab is cleared from the circulation through non-specific catabolism, no metabolic drug interactions are expected and no studies were done on routes of elimination.[3] The systemic clearance [rate] is about 0.2 L/day and the terminal half-life is about 25 days.[3]

Chemistry and manufacturing[edit]

Pembrolizumab is an immunoglobulin G4, with a variable region against the human programmed cell death 1 receptor, a humanized mouse monoclonal [228-L-proline(H10-S>P)]γ4 heavy chain (134-218') disulfide and a humanized mouse monoclonal κ light chain dimer (226-226:229-229)-bisdisulfide.[13]

It is recombinantly manufactured in Chinese hamster ovary (CHO) cells.[14]

History[edit]

Pembrolizumab was invented by scientists Gregory Carven, Hans van Eenennaam and John Dulos at Organon after which they worked with Medical Research Council Technology (now known as LifeArc) starting in 2006 to humanize the antibody; Schering-Plough acquired Organon in 2007 and Merck & Co. acquired Schering-Plough two years later.[15] Carven, van Eenennaam and Dulos were recognized as Inventors of the Year by the Intellectual Property Owners Education Foundation in 2016.[16]

The development program for pembrolizumab was seen as low priority at Organon, and then at Schering, and then at Merck, and in 2007 Merck terminated development and began preparing to outlicense it.[17] But then in 2010 scientists from Bristol Myers Squibb published a paper in the New England Journal of Medicine showing that their checkpoint inhibitor, ipilimumab (Yervoy) had shown strong promise in metastatic melanoma and that a second BMS checkpoint inhibitor, nivolumab, (Opdivo) was also promising.[17] Merck at that time has little commitment and expertise in oncology or immunotherapy, but understood the opportunity and reacted strongly, reactivating the program and filing its IND by the end of 2010.[17] As an example, Martin Huber was one of the few senior people at Merck with strong experience in lung cancer drug development, but had been promoted to senior management and was no longer involved in product development; he ended stepped down from his role and led clinical development of pembrolizumab for lung cancer.[17]

Scientists at the company argued for developing a companion diagnostic and limiting testing of the drug only to patients with biomarkers showing they were likely to respond; management agreed and some people, including shareholders and analysts, criticized this decision as it limited the potential market size for the drug, but others argued that it increased the chances of proving the drug would work, and made clinical trials faster and needing fewer patients due to the likelihood of greater effect size, and moving quickly and reducing the risk of failure was essential for catching up with BMS, which has an approximately five year lead over Merck.[17] The phase I study started in early 2011, and Eric Rubin, who was running the melanoma trial, argued for and was able to win expansion of the trial until it reached around 1300 people roughly divided between melanoma and lung cancer, the largest Phase I study ever run in oncology.[17] In 2013 Merck quietly applied for and won a Breakthrough therapy designation for the drug; this regulatory pathway was new at the time and not well understood; one of its advantages is that the FDA holds more frequent meetings with drug developers, which reduces the risk of developers making mistakes or misundertandings arising between regulator's expectations and what the developers want to do. This was Merck's first use of the designation and the reduction in regulatory risk was one of the reasons why management was willing to put company resources behind the development.[17]

In 2013, the USAN name was changed from lambrolizumab to pembrolizumab.[13] In that year clinical trial results in advanced melanoma were published in the New England Journal of Medicine.[18]

On September 4, 2014, the US Food and Drug Administration (FDA) approved pembrolizumab under the FDA Fast Track Development Program.[19] It is approved for use following treatment with ipilimumab, or after treatment with Ipilimumab and a BRAF inhibitor in advanced melanoma patients who carry a BRAF mutation.[20]

As of 2015, the only PDL-1 targeting drugs on the market were pembrolizumab and Bristol-Myers Squibb's Yervoy and Opdivo, and clinical developments in the class of drugs received coverage in the New York Times.[21]

By April 2016, Merck had applied for approval to market the drug in Japan and had signed an agreement with Taiho Pharmaceutical to co-promote it there.[22]

In July 2015, pembrolizumab received marketing approval in Europe.[3][23]

On October 2, 2015, the FDA approved pembrolizumab for the treatment of metastatic non-small cell lung cancer (NSCLC) in patients whose tumors express PD-L1 and who have failed treatment with other chemotherapeutic agents.[24]

In July 2016, the US FDA accepted for priority review an application for recurrent or metastatic head and neck squamous cell carcinoma (HNSCC) after a platinum-based chemotherapy,[25] and gave an accelerated approval on August 9, 2016.[26]

In August 2016, the FDA granted an accelerated approval to pembrolizumab as a treatment for patients with recurrent or metastatic head and neck squamous cell carcinoma (HNSCC) ("regardless of PD-L1 staining") following progression on a platinum-based chemotherapy, based on objective response rates (ORR) in the phase Ib KEYNOTE-012 study.[26] Full approval depends on the results of the phase III KEYNOTE-040 study (NCT02252042), running until Jan 2017.[26]

In May 2017, pembrolizumab received an accelerated approval from the FDA for use in any unresectable or metastatic solid tumor with DNA mismatch repair deficiencies or a microsatellite instability-high state (or, in the case of colon cancer, tumors that have progressed following chemotherapy). This approval marked the first instance in which the FDA approved marketing of a drug based only on the presence of a genetic mutation, with no limitation on the site of the cancer or the kind of tissue in which it originated. [27][12][28] The approval was based on a clinical trial of 149 patients with microsatellite instability-high or mismatch repair deficient cancers who enrolled on one of five single-arm trials. Ninety patients had colorectal cancer, and 59 patients had one of 14 other cancer types. The objective response rate for all patients was 39.6%. Response rates were similar across all cancer types, including 36% in colorectal cancer and 46% across the other tumor types. Notably, there were 11 complete responses, with the remainder partial responses. Responses lasted for at least six months in 78% of responders.[12] Because the clinical trial was fairly small, Merck was obligated to conduct further post-marketing studies to ensure that the results are valid.[29]

Society and culture[edit]

Pembrolizumab was priced at $150,000 per year when it launched (late 2014).[30]

Research[edit]

In 2015, Merck reported results in 13 cancer types; much attention was given to early results in head and neck cancer.[31][32]

As of May 2016, pembrolizumab was in Phase IB clinical trials for triple-negative breast cancer (TNBC), gastric cancer, urothelial cancer, and head and neck cancer (all under the "Keynote-012" trial) and in Phase II trial for TNBC (the "Keynote-086" trial).[33] At ASCO in June 2016, Merck reported that the clinical development program was directed to around 30 cancers and that it was running over 270 clinical trials (around 100 in combination with other treatments) and had four registration-enabling studies in process.[34]

Results of a Phase II clinical trial in Merkel-cell carcinoma were reported in the New England Journal of Medicine in June 2016.[35]

Results of a clinical trial in people with untreatable metastases arising from various solid tumors were published in Science in 2017.[36]

It is in a phase III trial in combination with epacadostat, an Indoleamine 2,3-dioxygenase (IDO1) inhibitor to treat melanoma.[37]

See also[edit]

References[edit]

 This article incorporates public domain material from the United States Department of Health and Human Services document "FDA D.I.S.C.O.: First Tissue/Site Agnostic Approval Transcript" by Sanjeeve Bala (retrieved on 2017-06-17).

  1. ^ "Pembrolizumab". AdisInsight. Retrieved 5 November 2016. 
  2. ^ a b c "Pembrolizumab label" (PDF). FDA. May 2017.  linked from Index page at FDA website November 2016
  3. ^ a b c d e f g h i j "Pembrolizumab label at eMC". UK Electronic Medicines Compendium. 27 January 2017. 
  4. ^ Redman, Jason M.; Gibney, Geoffrey T.; Atkins, Michael B. (6 February 2016). "Advances in immunotherapy for melanoma". BMC Medicine. 14 (1). PMC 4744430Freely accessible. PMID 26850630. doi:10.1186/s12916-016-0571-0. 
  5. ^ Fuereder, Thorsten (20 June 2016). "Immunotherapy for head and neck squamous cell carcinoma". memo - Magazine of European Medical Oncology. 9 (2): 66–69. PMC 4923082Freely accessible. PMID 27429658. doi:10.1007/s12254-016-0270-8. 
  6. ^ Pembrolizumab (KEYTRUDA) for classical Hodgkin lymphoma, 15 Mar 2017, FDA
  7. ^ Chen, Hongbin; Vachhani, Pankit (September 2016). "Spotlight on pembrolizumab in non-small cell lung cancer: the evidence to date". OncoTargets and Therapy. 9: 5855–5866. PMC 5045223Freely accessible. PMID 27713639. doi:10.2147/ott.s97746. 
  8. ^ Linardou, Helena; Gogas, Helen (July 2016). "Toxicity management of immunotherapy for patients with metastatic melanoma". Annals of Translational Medicine. 4 (14): 272–272. PMC 4971373Freely accessible. PMID 27563659. doi:10.21037/atm.2016.07.10. 
  9. ^ a b Francisco LM, Sage PT, Sharpe AH (Jul 2010). "The PD-1 pathway in tolerance and autoimmunity". Immunological Reviews. 236: 219–42. PMC 2919275Freely accessible. PMID 20636820. doi:10.1111/j.1600-065X.2010.00923.x. 
  10. ^ a b Buqué, Aitziber; et al. (2 March 2015). "Trial Watch: Immunomodulatory monoclonal antibodies for oncological indications". OncoImmunology. 4 (4): e1008814. PMC 4485728Freely accessible. PMID 26137403. doi:10.1080/2162402x.2015.1008814. 
  11. ^ Pardoll, DM (Mar 22, 2012). "The blockade of immune checkpoints in cancer immunotherapy". Nature Reviews Cancer. 12 (4): 252–64. PMC 4856023Freely accessible. PMID 22437870. doi:10.1038/nrc3239. 
  12. ^ a b c Bala, Sanjeeve; Nair, Abhi (30 May 2017). "Approved Drugs - FDA D.I.S.C.O.: First Tissue/Site Agnostic Approval Transcript". FDA. 
  13. ^ a b "Statement on a Nonproprietary Name Adopted by the USAN Council" (PDF). November 27, 2013. 
  14. ^ "Assessment report: Keytruda. Procedure No. EMEA/H/C/003820/0000" (PDF). EMA. 21 May 2015. 
  15. ^ "Unlocking Checkpoint Inhibition". Translational Scientist. August 9, 2016. 
  16. ^ "Inventors of the Year". Inventors Digest. Retrieved 8 May 2017. 
  17. ^ a b c d e f g Shaywitz, David (July 26, 2017). "The Startling History Behind Merck's New Cancer Blockbuster". Forbes. 
  18. ^ Hamid, O; et al. (2013). "Safety and tumor responses with lambrolizumab (anti-PD-1) in melanoma". New England Journal of Medicine. 369 (2): 134–44. PMC 4126516Freely accessible. PMID 23724846. doi:10.1056/nejmoa1305133. 
  19. ^ U.S. Food and Drug Administration (September 4, 2014). "FDA approves Keytruda for advanced melanoma". U.S. Food and Drug Administration. U.S. Food and Drug Administration. Retrieved 24 December 2015. 
  20. ^ "FDA Approves Anti-PD-1 Drug for Advanced Melanoma". cancernetwork.com. 
  21. ^ Pollack, Andrew (May 29, 2015). "New Class of Drugs Shows More Promise in Treating Cancer". New York Times. Retrieved May 30, 2015. 
  22. ^ "Merck & Co and Taiho to co-promote cancer immunotherapy pembrolizumab in Japan". The Pharma Letter. April 13, 2016. 
  23. ^ "Keytruda index page at EMA". European Medicines Agency. Retrieved 10 November 2016. 
  24. ^ "FDA approves Keytruda for advanced non-small cell lung cancer". U.S. Food and Drug Administration. October 2, 2015. 
  25. ^ Potential Biomarkers Identified for Pembrolizumab in Head and Neck Cancer. July 2016
  26. ^ a b c FDA Approves Pembrolizumab for Head and Neck Cancer. Aug 2016
  27. ^ "FDA grants accelerated approval to pembrolizumab for first tissue/site agnostic indication". Food and Drug Adminstration. May 23, 2017. 
  28. ^ Ledford, Heidi (24 May 2017). "News: Tissue-independent cancer drug gets fast-track approval from US regulator". Nature. doi:10.1038/nature.2017.22054. 
  29. ^ "Accelerated approval notice: BLA 125514/S-14" (PDF). FDA. 23 May 2017. 
  30. ^ Amgen slaps record-breaking $178K price on rare leukemia drug Blincyto
  31. ^ Timmerman, Luke (June 2, 2015). "ASCO Wrapup: Immunotherapy Shines, Hope For Brain Tumors, & The Great Cancer Drug Price Debate". Forbes. 
  32. ^ Adams, Katherine T. (24 July 2015). "Cancer Immunotherapies--and Their Cost--Take Center Stage at ASCO's 2015 Annual Meeting". Managed Care Magazine Online. 
  33. ^ Jenkins, Kristin (5 May 2016). "Keytruda Impresses in Triple-Negative Breast Cancer". MedPage Today. 
  34. ^ "Merck & Co updates Keytruda findings at ASCO". The Pharma Letter. June 10, 2016. 
  35. ^ Nghiem (2016). "PD-1 Blockade with Pembrolizumab in Advanced Merkel-Cell Carcinoma". N. Engl. J. Med. 374 (26): 2542–2552. PMC 4927341Freely accessible. PMID 27093365. doi:10.1056/NEJMoa1603702. 
  36. ^ Le, DT; Durham, JN; Smith, KN; Wang, H; Bartlett, BR; Aulakh, LK; Lu, S; Kemberling, H; Wilt, C; Luber, BS; Wong, F; Azad, NS; Rucki, AA; Laheru, D; Donehower, R; Zaheer, A; Fisher, GA; Crocenzi, TS; Lee, JJ; Greten, TF; Duffy, AG; Ciombor, KK; Eyring, AD; Lam, BH; Joe, A; Kang, SP; Holdhoff, M; Danilova, L; Cope, L; Meyer, C; Zhou, S; Goldberg, RM; Armstrong, DK; Bever, KM; Fader, AN; Taube, J; Housseau, F; Spetzler, D; Xiao, N; Pardoll, DM; Papadopoulos, N; Kinzler, KW; Eshleman, JR; Vogelstein, B; Anders, RA; Diaz LA, Jr (8 June 2017). "Mismatch-repair deficiency predicts response of solid tumors to PD-1 blockade.". Science (New York, N.Y.). PMID 28596308. doi:10.1126/science.aan6733. 
  37. ^ A Phase 3 Study of Pembrolizumab + Epacadostat or Placebo in Subjects With Unresectable or Metastatic Melanoma (Keynote-252 / ECHO-301)