Phosphopantothenate—cysteine ligase

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phosphopantothenate-cysteine ligase
Identifiers
EC number6.3.2.5
CAS number9023-50-1
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum
Gene OntologyAmiGO / QuickGO

In enzymology, a phosphopantothenate-cysteine ligase also known as phosphopantothenoylcysteine synthetase (PPCS) is an enzyme that catalyzes the chemical reaction which constitutes the second of five steps involved in the conversion of pantothenate to Coenzyme A. The reaction is:

NTP + (R)-4'-phosphopantothenate + L-cysteine NMP + diphosphate + N-[(R)-4'-phosphopantothenoyl]-L-cysteine

The nucleoside triphosphate (NTP) involved in the reaction varies from species to species. Phosphopantothenate—cysteine ligase from the bacterium Escherichia coli uses cytidine triphosphate (CTP) as an energy donor, whilst the human isoform uses adenosine triphosphate (ATP).[1]

Nomenclature[edit]

This enzyme belongs to the family of ligases, specifically those forming carbon-nitrogen bonds as acid-D-amino-acid ligases (peptide synthases). The systematic name of this enzyme class is (R)-4'-phosphopantothenate:L-cysteine ligase. This enzyme is also called phosphopantothenoylcysteine synthetase.

Gene[edit]

PPCS
Available structures
PDBOrtholog search: PDBe RCSB
Identifiers
AliasesPPCS, Phosphopantothenate—cysteine ligase, phosphopantothenoylcysteine synthetase, CMD2C
External IDsOMIM: 609853 MGI: 1915237 HomoloGene: 6173 GeneCards: PPCS
Gene location (Human)
Chromosome 1 (human)
Chr.Chromosome 1 (human)[2]
Chromosome 1 (human)
Genomic location for PPCS
Genomic location for PPCS
Band1p34.2Start42,456,117 bp[2]
End42,473,385 bp[2]
Orthologs
SpeciesHumanMouse
Entrez
Ensembl
UniProt
RefSeq (mRNA)

NM_026494
NM_001355214

RefSeq (protein)

NP_080770
NP_001342143

Location (UCSC)Chr 1: 42.46 – 42.47 MbChr 4: 119.42 – 119.42 Mb
PubMed search[4][5]
Wikidata
View/Edit HumanView/Edit Mouse

Phosphopantothenoylcysteine synthetase in humans is encoded by the PPCS gene.[6]

Protein structure[edit]

As of late 2007, 5 structures have been solved for this class of enzymes, with PDB accession codes 1P9O, 1U7U, 1U7W, 1U7Z, and 1U80.

References[edit]

  1. ^ Manoj N, Strauss E, Begley TP, Ealick SE (Aug 2003). "Structure of human phosphopantothenoylcysteine synthetase at 2.3 A resolution". Structure. 11 (8): 927–936. doi:10.1016/S0969-2126(03)00146-1. PMID 12906824.
  2. ^ a b c GRCh38: Ensembl release 89: ENSG00000127125 - Ensembl, May 2017
  3. ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000028636 - Ensembl, May 2017
  4. ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  6. ^ "Entrez Gene: Phosphopantothenoylcysteine synthetase".

Further reading[edit]

  • Brown GM (1959). "The metabolism of pantothenic acid". J. Biol. Chem. 234 (2): 370–8. PMID 13630913.
  • Strauss E, Kinsland C, Ge Y, McLafferty FW, Begley TP (2001). "Phosphopantothenoylcysteine synthetase from Escherichia coli Identification and characterization of the last unidentified coenzyme A biosynthetic enzyme in bacteria". J. Biol. Chem. 276 (17): 13513–6. doi:10.1074/jbc.C100033200. PMID 11278255.
  • Kupke T (2002). "Molecular characterization of the 4'-phosphopantothenoylcysteine synthetase domain of bacterial dfp flavoproteins". J. Biol. Chem. 277 (39): 36137–45. doi:10.1074/jbc.M206188200. PMID 12140293.

External links[edit]

  • PDBe-KB provides an overview of all the structure information available in the PDB for Human Phosphopantothenate—cysteine ligase