Portable water purification
Portable water purification devices – better described as point-of-use (POU) water treatment systems and field water disinfection techniques – are self-contained, hand-carried units used by recreational enthusiasts, military personnel, survivalists, and others for water purification when they need to obtain drinking water from untreated sources (e.g. rivers, lakes, groundwater etc.). These personal devices and methods attempt to render water potable (i.e. safe and palatable for drinking purposes - without disease-causing pathogens).
Techniques include heat (including boiling), filtration, activated charcoal absorption, chemical disinfection (e.g. chlorine, iodine, ozone, etc.), ultraviolet purification (including SODIS), distillation (including solar distillation), and flocculation. Often these are used in combination.
Many commercial portable water purification systems or chemical additives are available for hiking, camping, and other travel in remote areas.
- 1 Drinking water hazards
- 2 Techniques
- 2.1 Heat (Boiling)
- 2.2 Filtration
- 2.3 Activated charcoal absorption
- 2.4 Chemical Disinfection with Halogens
- 2.5 Other Chemical Disinfection Additives
- 2.6 Ultraviolet purification
- 2.7 Ozone water disinfection
- 2.8 Solar water disinfection
- 2.9 Solar distillation
- 2.10 Homemade water filters
- 3 Prevention of water contamination
- 4 See also
- 5 References
- 6 External links
Drinking water hazards
Untreated water may contain potentially pathogenic agents including protozoa, bacteria, viruses, and some larvae of higher order parasites such as liver flukes and round worms. Chemical pollutants such as pesticides, heavy metals and synthetic organics may be present. Other components may affect taste, odour and general aesthetic qualities including turbidity from soil or clay, colour from humic acid or microscopic algae, odours from certain type of bacteria, particularly Actinomycetes which produce geosmin, and saltiness from brackish or sea water.
Common metallic contaminants such as copper and lead can be treated by increasing the pH using soda ash or lime which precipitates such metals. Carefully decanting of the clear water after settlement or the use of filtration provides acceptably low levels of metals. Water contaminated by aluminium or zinc cannot to treated in this way using a strong alkali as higher pHs re-dissolve the metal salts. Salt is difficult to remove except by reverse osmosis or distillation.
Most portable treatment processes focus on mitigating human pathogens for safety and removing particulates matter, tastes and odours. Significant pathogens commonly present in the developed world include Giardia, Cryptosporidium, Shigella, hepatitis A virus, Escherichia coli, and enterovirus. In less developed countries there may be risks from Cholera and Dysentery organisms and a range of tropical enteroparasites.
Giardia lamblia and Cryptosporidium spp., both of which cause diarrhea (see giardiasis and cryptosporidiosis) are common pathogens. In backcountry areas of the United States and Canada they are sometimes present in sufficient quantity that water treatment is justified for backpackers, although this has created some controversy. (See wilderness acquired diarrhea.) In Hawaii and other tropical areas, Leptospira spp. are another possible problem.
Less commonly seen in developed countries are organisms such as Vibrio cholerae which causes cholera and various strains of Salmonella which cause typhoid and para-typhoid diseases. Pathogenic viruses may also be found in water. The larvae of flukes are particularly dangerous in area frequented by sheep, deer, or cattle. If such microscopic larvae are ingested, they can form potentially life-threatening cysts in the brain or liver. This risk extends to plants grown in or near water including the commonly eaten watercress.
In general, more human activity up stream (i.e. the larger the stream/river) the greater the potential for contamination from sewage effluent, surface runoff, or industrial pollutants. Groundwater pollution may occur from human activity (e.g. on-site sanitation systems or mining) or might be naturally occurring (e.g. from arsenic in some regions of India and Bangladesh). Water collected as far upstream as possible above all known or anticipated risks of pollution poses the lowest risk of contamination and is best suited to portable treatment methods.
Not all techniques by themselves will mitigate all hazards. Although flocculation followed by filtration has been suggested as best practice this is rarely practicable without the ability to carefully control pH and settling conditions.Ill advised used of alum as a flocculant can lead to unacceptable levels of aluminium in the water so treated. If water is to be stored, halogens offer extended protection.
Heat kills disease-causing micro-organisms, with higher temperatures and/or duration required for some pathogens. Sterilization of water (killing all living containments) is not necessary to make water safe to drink; one only needs to render harmless enteric (intestinal) pathogens. Boiling does not remove most pollutants and has the potential to concentrates toxins if extended over significant periods. Boiling does not leave any residual protection.
The CDC recommends a rolling boil for 1 minute. At high elevations, though, the boiling point of water drops. At altitudes greater than 6,562 feet (2000 meters) boiling should continue for 3 minutes.
All bacterial pathogens are quickly killed above 60 °C (140 °F), therefore, although boiling is not necessary to make the water safe to drink, the time taken to heat the water to boiling is usually sufficient to reduce bacterial concentrations to safe levels. Encysted protozoan pathogens may require higher temperatures to remove any risk.
Boiling is not always necessary nor sometimes enough. Pasteurization where enough pathogens are killed typically occurs at 63 °C for 30 minutes or 72 °C for 15 seconds. Certain pathogens must be heated above boiling (e.g. botulism – Clostridium botulinum requires 118 °C (244 °F), most endospores require 120 °C (248 °F), and prions even higher). Higher temperatures may be achieved with a pressure cooker. Heat combined with ultraviolet light (UV), such as SODIS method, reduces the necessary temperature + duration.
Portable pump filters are commercially available with ceramic filters that filter 5,000 to 50,000 litres per cartridge, removing pathogens down to the 0.2–0.3 micrometer (µm) range. Some also utilize activated charcoal filtering. Most filters of this kind remove most bacteria and protozoa, such as Cryptosporidium and Giardia lamblia, but not viruses except for the very largest of 0.3 µm and larger diameters, so disinfection by chemicals or ultraviolet light is still required after filtration. It is worth noting that not all bacteria are removed by 0.2 µm pump filters; for example, strands of thread-like Leptospira spp. (which can cause leptospirosis) are thin enough to pass through a 0.2 µm filter. Effective chemical additives to address shortcomings in pump filters include chlorine, chlorine dioxide, iodine, and sodium hypochlorite (bleach). There have been polymer and ceramic filters on the market that incorporated iodine post-treatment in their filter elements to kill viruses and the smaller bacteria that cannot be filtered out, but most have disappeared due to the unpleasant taste imparted to the water, as well as possible adverse health effects when iodine is ingested over protracted periods.
While the filtration elements may do an excellent job of removing most bacteria and fungi contaminants from drinking water when new, the elements themselves can become colonization sites. In recent years some filters have been enhanced by bonding silver metal nanoparticles to the ceramic element and/or to the activated charcoal to suppress growth of pathogens.
Small, hand-pumped reverse osmosis filters were originally developed for the military in the late 1980s for use as survival equipment, for example, to be included with inflatable rafts on aircraft. Civilian versions are available. Instead of using the static pressure of a water supply line to force the water through the filter, pressure is provided by a hand-operated pump, similar in function and appearance to a mechanic's grease gun. These devices can generate drinkable water from seawater.
The Portable Aqua Unit for Lifesaving (short PAUL) is a portable ultrafiltration based membrane water filter for humanitarian aid. It allows the decentralized supply of clean water in emergency and disaster situations for about 400 persons per unit per day. The filter is designed to function with neither chemicals nor energy nor trained personnel.
Activated charcoal absorption
Granular activated carbon filtering utilizes a form of activated carbon with a high surface area, and absorbs many compounds, including many toxic compounds. Water passing through activated carbon is commonly used in concert with hand pumped filters to address organic contamination, taste, or objectionable odors. Activated carbon filters aren't usually used as the primary purification techniques of portable water purification devices, but rather as secondary means to complement another purification technique. It is most commonly implemented for pre- or post-filtering, in a separate step than ceramic filtering, in either case being implemented prior to the addition of chemical disinfectants used to control bacteria or viruses that filters cannot remove. Activated charcoal can remove chlorine from treated water, removing any residual protection remaining in the water protecting against pathogens, and should not, in general, be used without careful thought after chemical disinfection treatments in portable water purification processing. Ceramic/Carbon Core filters with a 0.5 µm or smaller pore size are excellent for removing bacteria and cysts while also removing chemicals.
Chemical Disinfection with Halogens
Chemical disinfection with halogens, chiefly chlorine and iodine, results from oxidation of essential cellular structures and enzymes. The primary factors that determine the rate and proportion of microorganisms killed are the residual or available halogen concentration and the exposure time. Secondary factors are pathogen species, water temperature, pH, and organic contaminants. In field-water disinfection, use of concentrations of 1–16 mg/L for 10–60 min is generally effective. Of note, Cryptosporidium oocysts, likely Cyclospora species, Ascaris eggs are extremely resistant to halogens and field inactivation may not be practical with bleach and iodine.
Iodine used for water purification is commonly added to water as a solution, in crystallized form, or in tablets containing tetraglycine hydroperiodide that release 8 mg of iodine per tablet adaptation to chronic tetraglycine hydroperiodide. The iodine kills many, but not all, of the most common pathogens present in natural fresh water sources. Carrying iodine for water purification is an imperfect but lightweight solution for those in need of field purification of drinking water. Kits are available in camping stores that include an iodine pill and a second pill (vitamin C or ascorbic acid) that will remove the iodine taste from the water after it has been disinfected. The addition of vitamin C, in the form of a pill or in flavored drink powders, precipitates much of the iodine out of the solution, so it should not be added until the iodine has had sufficient time to work. This time is 30 minutes in relatively clear, warm water, but is considerably longer if the water is turbid or cold. Iodine treated drinking water, treated with tablets containing tetraglycine hydroperiodide, also reduces the uptake of radioactive iodine in human subjects to only 2% of the value it would otherwise be. This could be an important factor worthy of consideration for treating water in a recent post nuclear event survival situation, where radioactive iodine ingestion is a concern for internal radiotoxicity. If the iodine has precipitated out of the solution, then the drinking water has less available iodine in the solution. Also the amount of iodine in one tablet is not sufficient to block uptake. Tetraglycine hydroperiodide maintains its effectiveness indefinitely before the container is opened; although some manufacturers suggest not using the tablets more than three months after the container has initially been opened, the shelf life is in fact very long provided that the container is resealed immediately after each time it is opened.
Iodine should be allowed at least 30 minutes to kill Giardia.
A potentially lower cost alternative to using iodine-based water purification tablets is the use of iodine crystals. A small amount of water is poured into a small glass bottle (with a capacity of approximately 1 ounce or 30 cubic centimeters), containing approximately 0.25 ounces (7 grams) of iodine crystals, and then shaken vigorously to produce a saturated solution of iodine. At 25 °C (77 °F), 12.5 cubic centimeters of this solution is added to one liter of water to be disinfected. In 15 minutes the water may be consumed. At a temperature of 20 °C (68 °F) let the water to be consumed stand for 20 minutes. At a temperature of 3 °C (37 °F) let the water stand for 30 minutes or use 25 cubic centimeters of saturated solution per liter. (Do not store the saturated iodine solution in a plastic bottle as the iodine vapor will pass through the plastic and corrode steel that is in close proximity.) Note also that this method may not be adequate in killing Giardia cysts in cold water. One solution is to warm the water to be consumed to 20 °C (68 °F) before treatment. Take care not to consume iodine crystals; the amount of iodine in the saturated solution may be two to four times the lethal dose. Care must be taken to prevent the small glass bottle of iodine crystals covered with water from freezing in cold climates. An advantage of using iodine crystals is that only a small amount of iodine is dissolved from the iodine crystals at each use, giving this method of treating water a capability for treating very large amounts of water, around 2,000 liters (500 gallons), with but a small bottle of crystals. Unlike tetraglycine hydroperiodide tablets, iodine crystals have essentially an unlimited shelf life as long as they are not exposed to air for long periods of time and are kept under water. (Iodine crystals will sublimate if exposed to air for long periods of time.) The large quantity of water that can be purified with iodine crystals at low cost makes this technique especially cost effective for point of use or emergency water purification methods intended for use longer than the shelf life of tetraglycine hydroperiodide.
Chlorine-based halazone tablets were formerly popularly used for portable water purification. Chlorine in water is more than three times more effective as a disinfectant against Escherichia coli than iodine. Halazone tablets were thus commonly used during World War II by U.S. soldiers for portable water purification, even being included in accessory packs for C-rations until 1945. The primary limitation of halazone tablets was the very short usable life of opened bottles, typically 3 days or less, unlike iodine-based tablets which have a usable open bottle life of 3 months. Sodium dichloroisocyanurate (NaDCC) has largely displaced halazone tablets for the few remaining chlorine based water purification tablets available today. It is compressed with effervescent salts, usually adipic acid and sodium bicarbonate, to form rapidly dissolving tablets, diluted to 10 parts per million available chlorine (ppm av.cl) when drinking water is mildly contaminated and 20ppm when visibly contaminated. Chlorine bleach tablets give a more stable platform for disinfecting the water than liquid bleach (sodium hypochlorite) as the liquid version tends to degrade with age and give unregulated results unless assays are carried out – not practical on the spot. Still, despite chlorine-based halazone tablets falling from favor for portable water purification, chlorine-based bleach may nonetheless safely be used for short-term emergency water disinfection. Two drops of unscented 5% bleach can be added per liter or quart of clear water, then allowed to stand covered for 30 to 60 minutes. After this treatment, the water may be left open to reduce the chlorine smell and taste. Guidelines are available online for effective emergency use of bleach to render unsafe water potable. The Centers for Disease Control & Prevention (CDC) and Population Services International (PSI) promote a similar product (a 0.5% - 1.5% sodium hypochlorite solution) as part of their Safe Water System (SWS) strategy. The product is sold in developing countries under local brand names specifically for the purpose of disinfecting drinking water (CDC: SWSPSI: SWS).
The EPA recommends 2 drops of 8.25% sodium hypochlorite solution (regular, unscented chlorine bleach) mixed per 1 quart/liter water and let stand 30 minutes. 2 drops of 5% solution also suffices. Double the amount of bleach if the water is cloudy, colored, or very cold. Afterwards, the water should have a slight chlorine odor, if not repeat the dosage and let stand for another 15 minutes before use.
Neither chlorine (e.g., bleach) nor iodine alone is considered completely effective against Cryptosporidium, although they are partially effective against Giardia. Chlorine is considered slightly better against the latter. A more complete field solution that includes chemical disinfectants is to first filter the water, using a 0.2 µm ceramic cartridge pumped filter, followed by treatment with iodine or chlorine, thereby filtering out cryptosporidium, Giardia, and most bacteria, along with the larger viruses, while also using chemical disinfectant to address smaller viruses and bacteria that the filter cannot remove. This combination is also potentially more effective in some cases than even using portable electronic disinfection based on UV treatment, such as using a SteriPEN UV portable water purifier.
Chlorine dioxide can come from tablets or be created by mixing two chemicals together (e.g. Aqau Mira drops). Chlorine dioxide also leaves an unpleasant taste in the water, but nowhere near as bad as iodine. The cost of chlorine dioxide treatment is about four times higher than the cost of iodine treatment. Ref needed
Mixed Oxidant (MiOx)
A simple brine (salt + water) solution in an electrolytic reaction produces a powerful mixed oxidant disinfectant (mostly chlorine in the form of hypochlorous acid (HOCl) and some peroxide, ozone, chlorine dioxide) to inactivate viruses, bacteria, Giardia and Cryptosporidium killing 99.9% of all organisms without the need to filter. MiOx is considered stronger than chlorine.
Chlorine Tablets (NaDCC)
Sodium Dichloroisocyanurate or Troclosene Sodium more commonly shortened as NaDCC, is a form of chlorine used for disinfection. It is used by all major NGO's such as UNICEF to treat water in emergencies, and widely by social marketing organisations for household water treatment where household sources of water may not be safe.
NaDCC tablets are available in a range of concentrations to treat differing volumes of water to give the World Heath Organisations recommended 5ppm available chlorine. They are effervescent tablets allowing the tablet to dissolve in a matter of minutes.
Other Chemical Disinfection Additives
Silver ion tablets
An alternative to iodine-based preparations in some usage scenarios are silver ion/chlorine dioxide-based tablets or droplets. These solutions may disinfect water more effectively than iodine based techniques while leaving hardly any noticeable taste in the water in some usage scenarios. Silver ion/chlorine dioxide based disinfecting agents will kill Cryptosporidium and Giardia, if utilized correctly. The primary disadvantage of silver ion/chlorine dioxide based techniques is the long purification times (generally 30 minutes to 4 hours, depending on the formulation used). Another concern is the possible deposition and accumulation of silver compounds in various body tissues leading to a rare condition called argyria that results in a permanent, disfiguring, bluish-gray pigmentation of the skin, eyes, and mucous membranes.
One recent study has found that the wild Salmonella which would reproduce quickly during subsequent dark storage of solar-disinfected water could be controlled by the addition of just 10 parts per million of hydrogen peroxide.
Ultraviolet (UV) light induces the formation of covalent linkages on DNA and thereby prevents microbes from reproducing. Without reproduction, the microbes become far less dangerous. Germicidal UV-C light in the short wavelength range of 100–280 nm acts on thymine, one of the four base nucleotides in DNA. When a germicidal UV photon is absorbed by a thymine molecule that is adjacent to another thymine within the DNA strand, a covalent bond or dimer between the molecules is created. This thymine dimer prevents enzymes from "reading" the DNA and copying it, thus neutering the microbe. Still, there are limits to this technology. Water turbidity (i.e., the amount of suspended & colloidal solids contained in the water to be treated) must be low, such that the water is clear, for UV purification to work well - thus a pre-filter step might be necessary.
A concern with UV portable water purification is that some pathogens are hundreds of times less sensitive to UV light than others. Protozoan cysts were once believed to be among the least sensitive, however recent studies have proved otherwise, demonstrating that both Cryptosporidium and Giardia are deactivated by a UV dose of just 6 mJ/cm sq. However, EPA regulations and other studies show that it is viruses that are the limiting factor of UV treatment, requiring a 10-30 times greater dose of UV light than Giardia or Cryptosporidium. Studies have shown that UV doses at the levels provided by common portable UV units are effective at killing Giardia and that there was no evidence of repair and reactivation of the cysts.
Water treated with UV still has the microbes present in the water, only with their means for reproduction turned "off". In the event that such UV-treated water containing neutered microbes is exposed to visible light (specifically, wavelengths of light over 330-500 nm) for any significant period of time, a process known as photo reactivation can take place, where the possibility for repairing the damage in the bacteria's reproduction DNA arises, potentially rendering them once more capable of reproducing and causing disease. UV-treated water must therefore not be exposed to visible light for any significant period of time after UV treatment, before consumption, to avoid ingesting reactivated and dangerous microbes.
Ozone water disinfection
In ozone water disinfection, microbes are destroyed by ozone gas (O3) provided by an ozone generator. Common in Europe, ozone gas is now becoming widely adopted in the United States. It is emerging across a wide array of industries; from municipal water treatment plants, to food processing plants, to healthcare organizations. It is being adopted due to its ability to sanitize water and surfaces without wasting water, and because there are no by-products. When its job is done, ozone gas quickly degrades into oxygen. Ozone is more effective than chlorine in destroying viruses and bacteria.
In 1990, the Organic Foods Production Act (OFPA) identified aqueous ozone as a substance that is allowed for use in organic crop and livestock production. In 1997, it was approved by the FDA as an antimicrobial agent for use on food. In 2002, the FDA approved ozone for use on food contact areas and directly on food with its Generally Regarded as Safe (“GRAS”) designation.
Ozone is most commonly created by a process called “corona discharge”, which causes oxygen molecules (O2) to temporarily re-combine into ozone (O3). This gas is very unstable, and the 3rd oxygen molecule reacts with pathogens by penetrating the cell walls of bacteria and viruses. This destroys the organisms.
Ozone is effective against pollutants for the same reason; it will react with long-chain carbon (organic) molecules, and break them down into less complex (and typically less harmful) molecules through oxidation.
Advances in ozone generation techniques, coupled with filtration, make this a viable new portable water purification method.
Solar water disinfection
In solar water disinfection (SODIS), microbes are destroyed by temperature and UVA radiation provided by the sun. Water is placed in a transparent plastic PET bottle or plastic bag, oxygenated by shaking partially-filled capped bottles prior to filling the bottles all the way, and left in the sun for 6–24 hours atop a reflective surface.
Solar distillation relies on sunlight to warm and evaporate the water to be purified which then condenses and trickles into a container. In theory, a solar (condensation) still removes all pathogens, salts, metals, and most chemicals but in field practice the lack of clean components, easy contact with dirt, improvised construction, and disturbances result in cleaner, yet contaminated water.
Homemade water filters
Water filters can be made on-site using local materials such as sand and charcoal (e.g. from firewood burned in a special way). These filters are sometimes used by soldiers and outdoor enthusiasts. Due to their low cost they can be made and used by anyone. The reliability of such systems is highly variable. Such filters can do little, if anything, to mitigate germs and other harmful constituents and can give a false sense of security that the water so produced is potable. Water processed through an improvised filter should undergo secondary processing such as boiling to render it safe for consumption.
Prevention of water contamination
Human disease/illness usually come from other humans, thus human-derived materials (feces, medical waste, wash water, lawn chemicals, gasoline engines, garbage, etc.) should be kept far away from water sources. For example, bury human waste well away (>200 feet) from water sources to reduce contamination. In some wilderness areas is it recommended that all waste be packed up and carted out to a properly designated disposal point.
- Problem Organisms in Water: Identification and Treatment, 3rd Ed. (M7). Amewrican Waterworks Associan. 2004.
- Geldreich E. Drinking water microbiology—new directions toward water quality enhancement. Int J Food Microbiol 1989;9:295-312.
- Boulware DR, Forgey WW, Martin WJ (2003). "Medical risks of wilderness hiking". The American Journal of Medicine 114 (4): 288–93. doi:10.1016/S0002-9343(02)01494-8. PMID 12681456.
- Welch TP (2000). "Risk of giardiasis from consumption of wilderness water in North America: a systematic review of epidemiologic data". International Journal of Infectious Diseases 4 (2): 100–3. doi:10.1016/S1201-9712(00)90102-4. PMID 10737847.
- "What is Leptospirosis?" (PDF). Hawaii State Department of Health. September 2006. Retrieved 26 November 2009.
- Clayton D.B:date=1989. Water pollution at Lowermoore North Cornwall. Lowermoore incident health advisory committee, Cornwall District Health Authority. p. 22.
- Backer, H. Water Disinfection for International and Wilderness Traveler. Clinical Infectious Diseases. (2002) 34 (3): 355-364. Available from: http://cid.oxfordjournals.org/content/34/3/355.full
- Lawley R (1 January 2013). "Cryptosporidium". Food Safety Watch.
- Hoff J. Inactivation of microbial agents by chemical disinfectants. Cincinnati: US Environmental Protection Agency; 1986. EPA/600/2-86/067.
- LeMar HJ, Georgitis WJ, McDermott MT (1995). "Thyroid adaptation to chronic tetraglycine hydroperiodide water purification tablet use". Journal of Clinical Endocrinology and Metabolism 80 (1): 220–3. doi:10.1210/jcem.80.1.7829615. PMID 7829615.
- EQUIPPED TO SURVIVE (tm) - Repackaging Potable Aqua
- Kahn FH, Visscher BR (1975). "Water Disinfection in the Wilderness -- a simple, effective method of iodination". Western Journal of Medicine 122 (5): 450–3. PMC 1129772. PMID 165639.
- Jarroll EL Jr., Bingham AK, Meyer EA (1980). "Inability of an iodination method to destroy completely Giardia cysts in cold water". Western Journal of Medicine 132 (6): 567–9. PMC 1272173. PMID 7405206.
- Zemlyn S, Wilson WW, Hellweg PA (1981). "A caution on iodine water purification". Western Journal of Medicine 135 (2): 166–7. PMC 1273058. PMID 7281653.
- Koski TA, Stuart LS, Ortenzio LF (1966). "Comparison of Chlorine, Bromine, and Iodine as Disinfectants for Swimming Pool Water". Applied Microbiology 14 (2): 276–9. PMC 546668. PMID 4959984.
- "UNICEF - Progress on Drinking Water and Sanitation" (PDF).
- "Water Purification Tablets".
- "WHO - Guidelines for drinking-water quality, fourth edition".
- Sciacca F, Rengifo-Herrera JA, Wéthé J, Pulgarin C (2010-01-08). "Dramatic enhancement of solar disinfection (SODIS) of wild Salmonella sp. in PET bottles by H(2)O(2) addition on natural water of Burkina Faso containing dissolved iron". Chemosphere (epub ahead of print) 78 (9): 1186–91. doi:10.1016/j.chemosphere.2009.12.001. PMID 20060566.
- USEPA, Ultraviolet Disinfection Guidance Manual for the final LT2ESWTR, Nov 2006
- "National Primary Drinking Water Regulations: Long Term 2 Enhanced Surface Water Treatment Rule". Federal Register (U.S. Environmental Protection Agency) 71 (3): 783. 5 Jan 2006. Retrieved 17 Apr 2010.
- Mofidi AA, Meyer EA, Wallis PM, Chou CL, Meyer BP, Ramalinham S, Coffey BM (2002). "The effect of UV light on the inactivation of Giardia lamblia and Giardia muris cysts as determined by animal infectivity assay (P-2951-01)". Water Research 36 (8): 2098–108. doi:10.1016/S0043-1354(01)00412-2. PMID 12092585.
- Campbell AT, Wallis P (2002). "The effect of UV irradiation on human-derived Giardia lamblia cysts". Water Research 36 (4): 963–9. doi:10.1016/S0043-1354(01)00309-8. PMID 11848367.
- Linden KG, Shin GA, Faubert G, Cairns W, Sobsey MD (2002). "UV disinfection of Giardia lamblia cysts in water". Environmental Science and Technology 36 (11): 2519–22. doi:10.1021/es0113403. PMID 12075814.
- Qiu X, Sundin GW, Chai B, Tiedje JM (November 2004). "Survival of Shewanella oneidensis MR-1 after UV Radiation Exposure". Applied and Environmental Microbiology 70 (11): 6435–43. doi:10.1128/AEM.70.11.6435-6443.2004. PMC 525172. PMID 15528503.
- Sanitation section of http://www.cdc.gov/healthywater/drinking/travel/backcountry_water_treatment.html
- Household Water Treatment Knowledge on CAWST website