Anita van Eijk was born in Neuchâtel, Switzerland, the daughter of Leonardus Antonius van Eijk and J.C.M. van Eijk-Steens. She studied English and literature at Leiden University and afterwards communications at the University of Amsterdam. During her university years, Anita undertook an internship in London at the J. Walter Thompson advertising agency. She graduated in 1996. Anita was employed in 1997 by the Amsterdam branch of Bloomberg, and was subsequently sent to London for a stint in Sales/Marketing and Television Production. She then worked in the marketing department of auction house Christie’s. She spent her early childhood in Aix-en-Provence, France, before the family headed back to the Netherlands where Anita undertook her primary school education. The Princess moved to Singapore afterwards and studied at the United World College of South East Asia. Back in the Netherlands Anita completed an International Baccalaureate at the Rhineland Lyceum.
Van Eijk met Prince Pieter-Christiaan of Orange-Nassau, van Vollenhoven in London and the couple announced their engagement on 25 February 2005. Prince Pieter-Christiaan is the third son of Princess Margriet of the Netherlands and Pieter van Vollenhoven. They married in a civil ceremony on 25 August 2005 at the Het Loo Palace, Apeldoorn, which was followed by a religious ceremony on 27 August 2005 at the Grote of St. Jeroenskerk, currently known as Oude Jeroenskerk, in Noordwijk. On her marriage, Van Eijk acquired the courtesy title of Her Highness, Princess of Orange-Nassau, van Vollenhoven-van Eijk as a result of her husband's status as a Prince of Orange-Nassau. Since Prince Pieter-Christiaan did not seek parliamentary approval for his marriage, owing to the remote chance of his succession to the throne, he lost his place in line to the Dutch throne upon his marriage.
On the 9 June 2006, Prince Pieter-Christiaan and Princess Anita announced that they were expecting their first child. Princess Anita gave birth to a daughter, Emma Francisca Catharina van Vollenhoven, on 28 November 2006. The baby was born at 6:00 pm at the Onze Lieve Vrouwe Gasthuis in Amsterdam and weighed 3970 grams (8 lbs, 7 ounces). On the 15th of May 2008, the couple announced that they are expecting their second child at the end of November 2008. Princess Anita gave birth to a son on 19 November 2008. His full name is Pieter Anton Maurits Erik. He weighed 3745 grams and he was born on 1.32 am in The Hague. The family lives in Noordwijk.