This article has multiple issues. Please help improve it or discuss these issues on the talk page. (Learn how and when to remove these template messages)(Learn how and when to remove this template message)
Elongation factors are a set of proteins that function at the ribosome, during protein synthesis, to facilitate translational elongation from formation of the first to the last peptide bond of a growing polypeptide. Bacteria and eukaryotes use elongation factors that are largely homologous to each other, but with distinct structures (and different research nomenclatures).
Elongation is the most rapid step in translation. In bacteria it proceeds at a rate of 15 to 20 amino acids added per second (about 45-60 nucleotides read, per second). In eukaryotes the rate is about two amino acids per second (about 6 nucleotides read, per second). Elongation factors play a role in orchestrating the events of this process, and in ensuring the highly accuracy translation at these speeds.
Table of corresponding bacterial and eukaryotic/archael EFs
|EF-Tu||eEF-1 subunit α||mediates the entry of the aminoacyl tRNA into a free site of the ribosome.|
|EF-Ts||eEF-1 subunit βγ||serves as the guanine nucleotide exchange factor for EF-Tu, catalyzing the release of GDP from EF-Tu.|
|EF-G||eEF-2||catalyzes the translocation of the tRNA and mRNA down the ribosome at the end of each round of polypeptide elongation. Shaped like EF-Tu plus tRNA.|
|EF-P||EIF5A||stimulates peptide formation by catalyzing the first synthesis step between the first amino acid (N-formylmethionine/methionine) and the second amino acid.|
|Note that EIF5A, the archaeal and eukaryotic homolog to EF-P, is instead considered an initiation factor.|
In addition to their cytoplasmic machinery, eukaryotic mitochrondria and plastids have their own translation machineries, each with their own set of bacterial-type elongation factors. In humans, they include TUFM, TSFM, GFM1, GFM2.
As a target
Elongation factors are targets for the toxins of some pathogens. For instance, Corynebacterium diphtheriae produces its toxin, which alters protein function in the host by inactivating elongation factor (EF-2). This results in the pathology and symptoms associated with C. diphtheriae infection. Likewise, Pseudomonas aeruginosa exotoxin A inactivates EF-2.[verification needed]
- Alberts, B. et al. (2002). Molecular Biology of the Cell, 4th ed. New York: Garland Science. ISBN 0-8153-3218-1.[page needed]
- Berg, J. M. et al. (2002). Biochemistry, 5th ed. New York: W.H. Freeman and Company. ISBN 0-7167-3051-0.[page needed]
- Singh, B. D. (2002). Fundamentals of Genetics, New Delhi, India: Kalyani Publishers. ISBN 81-7663-109-4.[page needed]
- nobelprize.org Explaining the function of eukaryotic elongation factors
- Elongation+Factor at the US National Library of Medicine Medical Subject Headings (MeSH)
- Peptide+Elongation+Factor+G at the US National Library of Medicine Medical Subject Headings (MeSH)
- Peptide+Elongation+Factor+Tu at the US National Library of Medicine Medical Subject Headings (MeSH)
- EC 188.8.131.52