Protein S

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PROS1
Protein PROS1 PDB 1z6c.png
Available structures
PDB Ortholog search: PDBe RCSB
Identifiers
Aliases PROS1, PROS, PS21, PS22, PS23, PS24, PS25, PSA, THPH5, THPH6, protein S (alpha)
External IDs OMIM: 176880 MGI: 1095733 HomoloGene: 264 GeneCards: 5627
RNA expression pattern
PBB GE PROS1 207808 s at tn.png
More reference expression data
Orthologs
Species Human Mouse
Entrez
Ensembl
UniProt
RefSeq (mRNA)

NM_000313
NM_001314077

NM_011173

RefSeq (protein)

NP_000304.2
NP_001301006.1

NP_035303.1

Location (UCSC) Chr 3: 93.87 – 93.97 Mb Chr 16: 62.85 – 62.93 Mb
PubMed search [1] [2]
Wikidata
View/Edit Human View/Edit Mouse

Protein S (also known as S-Protein) is a vitamin K-dependent plasma glycoprotein synthesized in the endothelium. In the circulation, Protein S exists in two forms: a free form and a complex form bound to complement protein C4b-binding protein (C4BP). In humans, protein S is encoded by the PROS1 gene.[1][2]

Structure[edit]

Protein S is partly homologous to other vitamin K-dependent plasma coagulation proteins, such as protein C and factors VII, IX, and X. Similar to them, it has a Gla-domain and several EGF-like domains (four rather than two), but no serine protease domain. Instead, there is a large C-terminus domain that is homologous to plasma steroid hormone-binding proteins such as sex hormone-binding globulin and corticosteroid-binding globulin. It may play a role in the protein functions as either a cofactor for activated protein C (APC) or in binding C4BP.[3][4]

Additionally, protein S has a peptide between the Gla-domain and the EGF-like domain, that is cleaved by thrombin. The Gla and EGF-like domains stay connected after the cleavage by a disulfide bond. However, protein S loses its function as an APC cofactor following either this cleavage or binding C4BP.[5]

Function[edit]

The best characterized function of Protein S is its role in the anti coagulation pathway, where it functions as a cofactor to Protein C in the inactivation of Factors Va and VIIIa. Only the free form has cofactor activity.[6]

Protein S can bind to negatively charged phospholipids via the carboxylated GLA domain. This property allows Protein S to function in the removal of cells which are undergoing apoptosis. Apoptosis is a form of cell death that is used by the body to remove unwanted or damaged cells from tissues. Cells, which are apoptotic (i.e. in the process of apoptosis), no longer actively manage the distribution of phospholipids in their outer membrane and hence begin to display negatively charged phospholipids, such as phosphatidyl serine, on the cell surface. In healthy cells, an ATP (Adenosine triphosphate)-dependent enzyme removes these from the outer leaflet of the cell membrane. These negatively charged phospholipids are recognized by phagocytes such as macrophages. Protein S can bind to the negatively charged phospholipids and function as a bridging molecule between the apoptotic cell and the phagocyte. The bridging property of Protein S enhances the phagocytosis of the apoptotic cell, allowing it to be removed 'cleanly' without any symptoms of tissue damage such as inflammation occurring.

Protein S also binds to the nascent complement complex C5,6,7 and prevents this complex from inserting into a membrane. This function prevents the inappropriate activation of the complement system, which would cause uncontrolled systemic inflammation. In fact, Protein S was first discovered in 1977 in this role and it is named after the membrane site that it occupies in the complex.[7]

Pathology[edit]

Mutations in the PROS1 gene can lead to Protein S deficiency which is a rare blood disorder which can lead to an increased risk of thrombosis.[8][9]

Interactions[edit]

Protein S has been shown to interact with Factor V.[10][11]

See also[edit]

References[edit]

  1. ^ Lundwall A, Dackowski W, Cohen E, Shaffer M, Mahr A, Dahlbäck B, Stenflo J, Wydro R (September 1986). "Isolation and sequence of the cDNA for human protein S, a regulator of blood coagulation". Proc. Natl. Acad. Sci. U.S.A. 83 (18): 6716–20. doi:10.1073/pnas.83.18.6716. PMC 386580. PMID 2944113. 
  2. ^ Long GL, Marshall A, Gardner JC, Naylor SL (January 1988). "Genes for human vitamin K-dependent plasma proteins C and S are located on chromosomes 2 and 3, respectively". Somat. Cell Mol. Genet. 14 (1): 93–8. doi:10.1007/BF01535052. PMID 2829367. 
  3. ^ Stenflo J (1999). "Contributions of Gla and EGF-like domains to the function of vitamin K-dependent coagulation factors". Critical reviews in eukaryotic gene expression 9 (1): 59–88. PMID 10200912. 
  4. ^ Rosner W (Dec 1991). "Plasma steroid-binding proteins". Endocrinology and metabolism clinics of North America 20 (4): 697–720. PMID 1778174. 
  5. ^ Dahlbäck B, Lundwall A, Stenflo J (Jun 1986). "Primary structure of bovine vitamin K-dependent protein S". Proceedings of the National Academy of Sciences 83 (12): 4199–203. PMID 2940598. 
  6. ^ Castoldi E, Hackeng TM (September 2008). "Regulation of coagulation by protein S". Curr. Opin. Hematol. 15 (5): 529–36. doi:10.1097/MOH.0b013e328309ec97. PMID 18695379. 
  7. ^ Podack, Eckhard; Kolb, William; Müller-Eberhard, Hans (1977). "The SC5b-7 complex: formation, isolation, properties, and subunit composition.". J. Immunol. 199: 2024–2029. PMID 410885. 
  8. ^ Beauchamp NJ, Dykes AC, Parikh N, Campbell Tait R, Daly ME (June 2004). "The prevalence of, and molecular defects underlying, inherited protein S deficiency in the general population". Br. J. Haematol. 125 (5): 647–54. doi:10.1111/j.1365-2141.2004.04961.x. PMID 15147381. 
  9. ^ García de Frutos P, Fuentes-Prior P, Hurtado B, Sala N (September 2007). "Molecular basis of protein S deficiency". Thromb. Haemost. 98 (3): 543–56. doi:10.1160/th07-03-0199. PMID 17849042. 
  10. ^ Heeb, M J; Kojima Y; Rosing J; Tans G; Griffin J H (Dec 1999). "C-terminal residues 621-635 of protein S are essential for binding to factor Va". J. Biol. Chem. (UNITED STATES) 274 (51): 36187–92. doi:10.1074/jbc.274.51.36187. ISSN 0021-9258. PMID 10593904. 
  11. ^ Heeb, M J; Mesters R M; Tans G; Rosing J; Griffin J H (Feb 1993). "Binding of protein S to factor Va associated with inhibition of prothrombinase that is independent of activated protein C". J. Biol. Chem. (UNITED STATES) 268 (4): 2872–7. ISSN 0021-9258. PMID 8428962. 

Further reading[edit]