Pyridoxal 5'-phosphate synthase (glutamine hydrolyzing)

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Pyridoxal 5'-phosphate synthase (glutamine hydrolyzing)
Identifiers
EC number 4.3.3.6
Databases
IntEnz IntEnz view
BRENDA BRENDA entry
ExPASy NiceZyme view
KEGG KEGG entry
MetaCyc metabolic pathway
PRIAM profile
PDB structures RCSB PDB PDBe PDBsum

Pyridoxal 5'-phosphate synthase (glutamine hydrolyzing) (EC 4.3.3.6, PdxST) is an enzyme with systematic name D-ribose 5-phosphate,D-glyceraldehyde 3-phosphate pyridoxal 5'-phosphate-lyase.[1][2][3][4][5][6][7][8] This enzyme catalyses the following chemical reaction

D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine pyridoxal 5'-phosphate + L-glutamate + 3 H2O + phosphate (overall reaction)
(1a) L-glutamine + H2O L-glutamate + NH3
(1b) D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + NH3 pyridoxal 5'-phosphate + 4 H2O + phosphate

The enzyme can also use ribulose 5-phosphate and dihydroxyacetone phosphate.

References[edit]

  1. ^ Burns, K.E.; Xiang, Y.; Kinsland, C.L.; McLafferty, F.W.; Begley, T.P. (2005). "Reconstitution and biochemical characterization of a new pyridoxal-5′-phosphate biosynthetic pathway". J. Am. Chem. Soc. 127: 3682–3683. doi:10.1021/ja042792t. PMID 15771487. 
  2. ^ Raschle, T.; Amrhein, N.; Fitzpatrick, T.B. (2005). "On the two components of pyridoxal 5′-phosphate synthase from Bacillus subtilis". J. Biol. Chem. 280: 32291–32300. doi:10.1074/jbc.m501356200. PMID 16030023. 
  3. ^ Strohmeier, M.; Raschle, T.; Mazurkiewicz, J.; Rippe, K.; Sinning, I.; Fitzpatrick, T.B.; Tews, I. (2006). "Structure of a bacterial pyridoxal 5′-phosphate synthase complex". Proc. Natl. Acad. Sci. USA. 103: 19284–19289. doi:10.1073/pnas.0604950103. PMC 1748218Freely accessible. PMID 17159152. 
  4. ^ Raschle, T.; Arigoni, D.; Brunisholz, R.; Rechsteiner, H.; Amrhein, N.; Fitzpatrick, T.B. (2007). "Reaction mechanism of pyridoxal 5′-phosphate synthase. Detection of an enzyme-bound chromophoric intermediate". J. Biol. Chem. 282: 6098–6105. doi:10.1074/jbc.m610614200. PMID 17189272. 
  5. ^ Hanes, J.W.; Keresztes, I.; Begley, T.P. (2008). "Trapping of a chromophoric intermediate in the Pdx1-catalyzed biosynthesis of pyridoxal 5′-phosphate". Angew. Chem. Int. Ed. Engl. 47: 2102–2105. doi:10.1002/anie.200704390. PMID 18260082. 
  6. ^ Hanes, J.W.; Burns, K.E.; Hilmey, D.G.; Chatterjee, A.; Dorrestein, P.C.; Begley, T.P. (2008). "Mechanistic studies on pyridoxal phosphate synthase: the reaction pathway leading to a chromophoric intermediate". J. Am. Chem. Soc. 130: 3043–3052. doi:10.1021/ja076604l. PMID 18271580. 
  7. ^ Hanes, J.W.; Keresztes, I.; Begley, T.P. (2008). "13C NMR snapshots of the complex reaction coordinate of pyridoxal phosphate synthase". Nat. Chem. Biol. 4: 425–430. doi:10.1038/nchembio.93. PMID 18516049. 
  8. ^ Wallner, S.; Neuwirth, M.; Flicker, K.; Tews, I.; Macheroux, P. (2009). "Dissection of contributions from invariant amino acids to complex formation and catalysis in the heteromeric pyridoxal 5-phosphate synthase complex from Bacillus subtilis". Biochemistry. 48: 1928–1935. doi:10.1021/bi801887r. PMID 19152323. 

External links[edit]