Pyridoxal 5'-phosphate synthase (glutamine hydrolyzing)

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Pyridoxal 5'-phosphate synthase (glutamine hydrolyzing)
EC number
IntEnz IntEnz view
ExPASy NiceZyme view
MetaCyc metabolic pathway
PRIAM profile
PDB structures RCSB PDB PDBe PDBsum

Pyridoxal 5'-phosphate synthase (glutamine hydrolyzing) (EC, PdxST) is an enzyme with systematic name D-ribose 5-phosphate,D-glyceraldehyde 3-phosphate pyridoxal 5'-phosphate-lyase.[1][2][3][4][5][6][7][8] This enzyme catalyses the following chemical reaction

D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine pyridoxal 5'-phosphate + L-glutamate + 3 H2O + phosphate (overall reaction)
(1a) L-glutamine + H2O L-glutamate + NH3
(1b) D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + NH3 pyridoxal 5'-phosphate + 4 H2O + phosphate

The enzyme can also use ribulose 5-phosphate and dihydroxyacetone phosphate.


  1. ^ Burns, K.E.; Xiang, Y.; Kinsland, C.L.; McLafferty, F.W.; Begley, T.P. (2005). "Reconstitution and biochemical characterization of a new pyridoxal-5′-phosphate biosynthetic pathway". J. Am. Chem. Soc. 127: 3682–3683. doi:10.1021/ja042792t. PMID 15771487. 
  2. ^ Raschle, T.; Amrhein, N.; Fitzpatrick, T.B. (2005). "On the two components of pyridoxal 5′-phosphate synthase from Bacillus subtilis". J. Biol. Chem. 280: 32291–32300. doi:10.1074/jbc.m501356200. PMID 16030023. 
  3. ^ Strohmeier, M.; Raschle, T.; Mazurkiewicz, J.; Rippe, K.; Sinning, I.; Fitzpatrick, T.B.; Tews, I. (2006). "Structure of a bacterial pyridoxal 5′-phosphate synthase complex". Proc. Natl. Acad. Sci. USA. 103: 19284–19289. doi:10.1073/pnas.0604950103. PMC 1748218Freely accessible. PMID 17159152. 
  4. ^ Raschle, T.; Arigoni, D.; Brunisholz, R.; Rechsteiner, H.; Amrhein, N.; Fitzpatrick, T.B. (2007). "Reaction mechanism of pyridoxal 5′-phosphate synthase. Detection of an enzyme-bound chromophoric intermediate". J. Biol. Chem. 282: 6098–6105. doi:10.1074/jbc.m610614200. PMID 17189272. 
  5. ^ Hanes, J.W.; Keresztes, I.; Begley, T.P. (2008). "Trapping of a chromophoric intermediate in the Pdx1-catalyzed biosynthesis of pyridoxal 5′-phosphate". Angew. Chem. Int. Ed. Engl. 47: 2102–2105. doi:10.1002/anie.200704390. PMID 18260082. 
  6. ^ Hanes, J.W.; Burns, K.E.; Hilmey, D.G.; Chatterjee, A.; Dorrestein, P.C.; Begley, T.P. (2008). "Mechanistic studies on pyridoxal phosphate synthase: the reaction pathway leading to a chromophoric intermediate". J. Am. Chem. Soc. 130: 3043–3052. doi:10.1021/ja076604l. PMID 18271580. 
  7. ^ Hanes, J.W.; Keresztes, I.; Begley, T.P. (2008). "13C NMR snapshots of the complex reaction coordinate of pyridoxal phosphate synthase". Nat. Chem. Biol. 4: 425–430. doi:10.1038/nchembio.93. PMID 18516049. 
  8. ^ Wallner, S.; Neuwirth, M.; Flicker, K.; Tews, I.; Macheroux, P. (2009). "Dissection of contributions from invariant amino acids to complex formation and catalysis in the heteromeric pyridoxal 5-phosphate synthase complex from Bacillus subtilis". Biochemistry. 48: 1928–1935. doi:10.1021/bi801887r. PMID 19152323. 

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