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REV1, polymerase (DNA directed)
Protein REV1 PDB 2EBW.png
Rendering based on PDB 2EBW​.
Available structures
PDB Ortholog search: PDBe, RCSB
Symbols REV1 ; REV1L
External IDs OMIM606134 MGI1929074 HomoloGene32309 GeneCards: REV1 Gene
RNA expression pattern
PBB GE REV1 218428 s at tn.png
More reference expression data
Species Human Mouse
Entrez 51455 56210
Ensembl ENSG00000135945 ENSMUSG00000026082
UniProt Q9UBZ9 Q920Q2
RefSeq (mRNA) NM_001037872 NM_019570
RefSeq (protein) NP_001032961 NP_062516
Location (UCSC) Chr 2:
99.4 – 99.49 Mb
Chr 1:
38.05 – 38.13 Mb
PubMed search [1] [2]

DNA repair protein REV1 is a protein that in humans is encoded by the REV1 gene.[1][2] This gene encodes a protein with similarity to the S. cerevisiae mutagenesis protein Rev1. The Rev1 proteins contain a BRCT domain, which is important in protein-protein interactions. A suggested role for the human Rev1-like protein is as a scaffold that recruits DNA polymerases involved in translesion synthesis (TLS) of damaged DNA. Two alternatively spliced transcript variants that encode different proteins have been found.[2] Rev1 is a Y family DNA polymerase; it is sometimes referred to as a deoxycytidyl transferase because it only inserts deoxycytidine (dC) across from lesions. Whether G, A, T, C, or an abasic site, Rev1 will always add a C. Rev1 has the ability to always add a C, because it uses an arginine as a template which complements well with C.[3] Yet it is believed[by whom?] that Rev1 rarely uses its polymerase activity, rather it is thought that Rev1's primary role is as a protein landing pad, whereby it helps direct the recruitment of TLS proteins, especially Pol ζ (Rev3/Rev7).


REV1 has been shown to interact with MAD2L2.[4] It is believed that Rev1 may interact with PCNA, once ubiquitylated due to a lesion, and help recruit Pol ζ (Rev3/Rev7) a B family polymerase involved in TLS.


  1. ^ Lin W, Xin H, Zhang Y, Wu X, Yuan F, Wang Z (Dec 1999). "The human REV1 gene codes for a DNA template-dependent dCMP transferase". Nucleic Acids Res 27 (22): 4468–75. doi:10.1093/nar/27.22.4468. PMC 148731. PMID 10536157. 
  2. ^ a b "Entrez Gene: REV1 REV1 homolog (S. cerevisiae)". 
  3. ^ Nair, DT (Sep 30, 2005). "Rev1 employs a novel mechanism of DNA synthesis using a protein template". Science. doi:10.1126/science.1116336. PMID 16195463. Retrieved Feb 6, 2015. 
  4. ^ Murakumo, Y; Ogura Y; Ishii H; Numata S; Ichihara M; Croce C M; Fishel R; Takahashi M (September 2001). "Interactions in the error-prone postreplication repair proteins hREV1, hREV3, and hREV7". J. Biol. Chem. (United States) 276 (38): 35644–51. doi:10.1074/jbc.M102051200. ISSN 0021-9258. PMID 11485998. 

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