Intrinsic termination (also called Rho-independent termination) is a mechanism in prokaryotes that causes RNA transcription to be stopped. In this mechanism, the mRNA contains a sequence that can base pair with itself to form a stem-loop structure 7-20 base pairs in length that is also rich in cytosine-guanine base pairs. C-G base pairs have significant base-stacking interactions (especially repeated G-C pairs) and can form three hydrogen bonds between each other, resulting in a stable RNA duplex. Following the stem-loop structure is a chain of uracil residues. The bonds between uracil and adenine are very weak. A protein bound to RNA polymerase (nusA) binds to the stem-loop structure tightly enough to cause the polymerase to temporarily stall. This pausing of the polymerase coincides with transcription of the poly-uracil sequence. The weak Adenine-Uracil bonds lower the energy of destabilization for the RNA-DNA duplex, allowing it to unwind and dissociate from the RNA polymerase.
Stem-loop structures that are not followed by a poly-Uracil sequence cause the RNA polymerase to pause, but it will typically continue transcription after a brief time because the duplex is too stable to unwind far enough to cause termination.
Rho-independent transcription termination is a frequent mechanism underlying the activity of cis-acting RNA regulatory elements, such as riboswitches.
- Farnham, PJ; Platt, T (1981-02-11). "Rho-independent termination: dyad symmetry in DNA causes RNA polymerase to pause during transcription in vitro.". Nucleic Acids Research. 9 (3): 563–77. doi:10.1093/nar/9.3.563. PMC . PMID 7012794.
- Lewin, Benjamin (2007). Genes IX. Sudbury, MA: Jones and Bartlett Publishers. ISBN 0-7637-4063-2.
- Wilson KS, von Hippel PH (September 1995). "Transcription termination at intrinsic terminators: the role of the RNA hairpin". Proc. Natl. Acad. Sci. U.S.A. 92 (19): 8793–7. doi:10.1073/pnas.92.19.8793. PMC . PMID 7568019. Retrieved 2010-09-20.