Rhodamine 610, C.I. Pigment Violet 1, Basic Violet 10, C.I. 45170
3D model (JSmol)
|Appearance||red to violet powder|
|Melting point||210 to 211 °C (410 to 412 °F; 483 to 484 K) (Decomposes)|
|~15 g/L (20 °C)|
|Safety data sheet||MSDS|
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
|what is ?)(|
Rhodamine B // is a chemical compound and a dye. It is often used as a tracer dye in water to determine the rate and direction of flow and transport. Rhodamine dyes fluoresce and can thus be detected easily and inexpensively with fluorometers. Rhodamine dyes are used extensively in biotechnology applications such as fluorescence microscopy, flow cytometry, fluorescence correlation spectroscopy and ELISA.
Rhodamine B is tunable around 610 nm when used as a laser dye. Its luminescence quantum yield is 0.65 in basic ethanol, 0.49 in ethanol, 1.0, and 0.68 in 94% ethanol. The fluorescence yield is temperature dependent.
The solubility of rhodamine B in water is ~15 g/L. However, the solubility in acetic acid solution (30 vol%) is ~400 g/L. However, the solubility of rhodamine B in water is reported as 0.8 g/100 mL and 1.5 g/100 mL in ethanol, which is more consistent with solubility in water and ethanol. Chlorinated tap water decomposes rhodamine B. Rhodamine B solutions adsorb to plastics and should be kept in glass.
Rhodamine B is being tested for use as a biomarker in oral rabies vaccines for wildlife, such as raccoons, to identify animals that have eaten a vaccine bait. The rhodamine is incorporated into the animal's whiskers and teeth.
It is also often mixed with herbicides to show where they have been used.
Rhodamine B (BV10) is mixed with Quinacridone Magenta (PR122) to make the bright pink watercolor known as Opera Rose.
Safety and health
In California, rhodamine B is suspected to be carcinogenic and thus products containing it must contain a warning on its label. Cases of economically motivated adulteration, where it has been illegally used to impart a red color to chili powder, have come to the attention of food safety regulators.
- Sicherheitsdatenblatt Rhodamin B by Roth, 2013
- Rhodamine B
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- Reagent and Dye Solubility Chart by Ellis, 2011
- Detection and prevention of leaks from dams By Antonio Plata Bedmar and Luís Araguás Araguás, Taylor & Francis, 2002, ISBN 90-5809-355-7
- Slate, Dennis; Algeo, Timothy P.; Nelson, Kathleen M.; Chipman, Richard B.; Donovan, Dennis; Blanton, Jesse D.; Niezgoda, Michael; Rupprecht, Charles E. (2009). Bethony, Jeffrey M., ed. "Oral Rabies Vaccination in North America: Opportunities, Complexities, and Challenges". PLoS Neglected Tropical Diseases. 3 (12): e549. doi:10.1371/journal.pntd.0000549. PMC 2791170. PMID 20027214.
- "Handprint: colormaking attributes". www.handprint.com.
- Naval Jelly MSDS with Rhodamine B
- Lin, Shuang (2015). "Rapid and sensitive SERS method for determination of Rhodamine B in chili powder with paper-based substrates" (PDF). Analytical Methods. 7: 5289. doi:10.1039/c5ay00028a. Retrieved 1 February 2018.