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Symbol rncO
Rfam RF00552
Other data
RNA type Cis-reg
Domain(s) Bacteria
SO 0000233

RncO is a bacterial non-coding RNA regulatory element found in the rnc leader sequence. The rnc operon is negatively auto-regulated by transcript stability. rnc, the first gene in the operon codes for RNase III which cleaves the long rncO stem II leading to transcript degradation and a reduction in translation.[1] Matsunaga et al. showed that RNase III cleavage can initiate rnc transcript decay independently of rnc gene translation.[2] Further work has established that rncO structure and function is conserved in Salmonella typhimurium.[3]


Functionally the first 215 nucleotides of the rnc leader have been shown to be sufficient. Within this region three stem-loops were identified. Stem-loop II is cleaved by RNase III, whereas stem-loops I and III are important for stability.[1]


  1. ^ a b Matsunaga J, Simons EL, Simons RW (1996). "RNase III autoregulation: structure and function of rncO, the posttranscriptional "operator"". RNA. 2 (12): 1228–40. PMC 1369450Freely accessible. PMID 8972772. 
  2. ^ Matsunaga J, Simons EL, Simons RW (1997). "Escherichia coli RNase III (rnc) autoregulation occurs independently of rnc gene translation". Mol. Microbiol. 26 (5): 1125–35. doi:10.1046/j.1365-2958.1997.6652007.x. PMID 9426147. 
  3. ^ Anderson PE, Matsunaga J, Simons EL, Simons RW (1996). "Structure and regulation of the Salmonella typhimurium rnc-era-recO operon". Biochimie. 78 (11–12): 1025–34. doi:10.1016/S0300-9084(97)86726-0. PMID 9150881. 

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