Roswell Park Memorial Institute medium
Roswell Park Memorial Institute medium, commonly referred to as RPMI medium or RPMI 1640, is a form of medium used in cell culture and tissue culture used for growing a variety of mammalian cell lines. It has traditionally been used for growth of human lymphocytes. This medium contains a great deal of phosphate and is formulated for use in a 5% carbon dioxide atmosphere. RPMI 1640 has traditionally been used for the serum-free expansion of human lymphoid cells.
RPMI 1640 uses a bicarbonate buffering system and differs from most mammalian cell culture media in its typical pH 8 formulation. Properly supplemented with serum or an adequate serum replacement, RPMI 1640 allows the cultivation of many cell types, especially human T/B-lymphocytes, bone marrow cells, and hybridoma cells.
The name is derived from Roswell Park Memorial Institute, where it was developed.
One liter of RPMI 1640 contains:
- Glucose (2 g)
- pH indicator (phenol red, 5 mg)
- Salts (6 g sodium chloride, 2 g sodium bicarbonate, 1.512 g disodium phosphate, 400 mg potassium chloride, 100 mg magnesium sulfate, and 100 mg calcium nitrate)
- Amino acids (300 mg glutamine; 200 mg arginine; 50 mg each asparagine, cystine, leucine, and isoleucine; 40 mg lysine hydrochloride; 30 mg serine; 20 mg each aspartic acid, glutamic acid, hydroxyproline, proline, threonine, tyrosine, and valine; 15 mg each histidine, methionine, and phenylalanine; 10 mg glycine; 5 mg tryptophan; and 1 mg reduced glutathione)
- Vitamins (35 mg i-inositol; 3 mg choline chloride; 1 mg each para-aminobenzoic acid, folic acid, nicotinamide, pyridoxine hydrochloride, and thiamine hydrochloride; 0.25 mg calcium pantothenate; 0.2 mg each biotin and riboflavin; and 0.005 mg cyanocobalamin)
- Atlas RM, Snyder JW (2006). Handbook of Media for Clinical Microbiology (2nd ed.). Boca Raton, Florida: CRC Press. p. 427.
- Moore GE, Gerner RE, Franklin HA (1967). "Culture of normal human leukocytes". JAMA. 199 (8): 519–524. PMID 4960081.
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