Roswell Park Memorial Institute medium

From Wikipedia, the free encyclopedia
Jump to navigation Jump to search

Roswell Park Memorial Institute medium, commonly referred to as RPMI medium or RPMI 1640, is a form of medium used in cell culture and tissue culture used for growing a variety of mammalian cell lines.[1] It has traditionally been used for growth of human lymphocytes. This medium contains a great deal of phosphate and is formulated for use in a 5% carbon dioxide atmosphere. RPMI 1640 has traditionally been used for the serum-free expansion of human lymphoid cells.

RPMI 1640 uses a bicarbonate buffering system and differs from most mammalian cell culture media in its typical pH 8 formulation. Properly supplemented with serum or an adequate serum replacement, RPMI 1640 allows the cultivation of many cell types, especially human T/B-lymphocytes, bone marrow cells, and hybridoma cells.

The original RPMI 1640 formulation was published by Moore et al.[2] and can be found online.[3]

The name is derived from Roswell Park Memorial Institute, where it was developed.


One liter of RPMI 1640 contains:[2]

  • Glucose (2 g)
  • pH indicator (phenol red, 5 mg)
  • Salts (6 g sodium chloride, 2 g sodium bicarbonate, 1.512 g disodium phosphate, 400 mg potassium chloride, 100 mg magnesium sulfate, and 100 mg calcium nitrate)
  • Amino acids (300 mg glutamine; 200 mg arginine; 50 mg each asparagine, cystine, leucine, and isoleucine; 40 mg lysine hydrochloride; 30 mg serine; 20 mg each aspartic acid, glutamic acid, hydroxyproline, proline, threonine, tyrosine, and valine; 15 mg each histidine, methionine, and phenylalanine; 10 mg glycine; 5 mg tryptophan; and 1 mg reduced glutathione)
  • Vitamins (35 mg i-inositol; 3 mg choline chloride; 1 mg each para-aminobenzoic acid, folic acid, nicotinamide, pyridoxine hydrochloride, and thiamine hydrochloride; 0.25 mg calcium pantothenate; 0.2 mg each biotin and riboflavin; and 0.005 mg cyanocobalamin)


  1. ^ Atlas RM, Snyder JW (2006). Handbook of Media for Clinical Microbiology (2nd ed.). Boca Raton, Florida: CRC Press. p. 427.
  2. ^ a b Moore GE, Gerner RE, Franklin HA (1967). "Culture of normal human leukocytes". JAMA. 199 (8): 519–524. PMID 4960081.
  3. ^

External links[edit]