38641-94-0 (isopropylammmonium salt)
70393-85-0 (sesquisodium salt)
81591-81-3 (trimethylsulfonium salt)
|Jmol 3D model||Interactive image|
|Molar mass||169.07 g·mol−1|
|Appearance||white crystalline powder|
|Density||1.704 (20 °C)|
|Melting point||184.5 °C (364.1 °F; 457.6 K)|
|Boiling point||decomposes at 187 °C (369 °F; 460 K)|
|1.01 g/100 mL (20 °C)|
|Acidity (pKa)||<2, 2.6, 5.6, 10.6|
|Safety data sheet||InChem MSDS|
|GHS signal word||DANGER|
|P273, P280, P305+351+338, P310, P501|
EU classification (DSD)
Dangerous for the environment (N)
|S-phrases||(S2), S26, S39, S61|
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
|what is ?)(|
Glyphosate (N-(phosphonomethyl)glycine) is a broad-spectrum systemic herbicide and crop desiccant. It is an organophosphorus compound, specifically a phosphonate. It is used to kill weeds, especially annual broadleaf weeds and grasses that compete with crops. It was discovered to be an herbicide by Monsanto chemist John E. Franz in 1970. Monsanto brought it to market in 1974 under the trade name Roundup, and Monsanto's last commercially relevant United States patent expired in 2000.
Farmers quickly adopted glyphosate, especially after Monsanto introduced glyphosate-resistant Roundup Ready crops, enabling farmers to kill weeds without killing their crops. In 2007, glyphosate was the most used herbicide in the United States' agricultural sector and the second-most used in home and garden, government and industry, and commerce. By 2016 there was a 100-fold increase from the late 1970s in the frequency of applications and volumes of glyphosate-based herbicides (GBHs) applied, partly in response to the unprecedented global emergence and spread of glyphosate-resistant weeds.:1
Glyphosate is absorbed through foliage, and minimally through roots, and transported to growing points. It inhibits a plant enzyme involved in the synthesis of three aromatic amino acids: tyrosine, tryptophan, and phenylalanine. Therefore, it is effective only on actively growing plants and is not effective as a pre-emergence herbicide. An increasing number of crops have been genetically engineered to be tolerant of glyphosate (e.g. Roundup Ready soybean, the first Roundup Ready crop, also created by Monsanto) which allows farmers to use glyphosate as a postemergence herbicide against weeds. The development of glyphosate resistance in weed species is emerging as a costly problem. While glyphosate and formulations such as Roundup have been approved by regulatory bodies worldwide, concerns about their effects on humans and the environment persist.
Many regulatory and scholarly reviews have evaluated the relative toxicity of glyphosate as an herbicide. The German Federal Institute for Risk Assessment toxicology review in 2013 found that "the available data is contradictory and far from being convincing" with regard to correlations between exposure to glyphosate formulations and risk of various cancers, including non-Hodgkin lymphoma (NHL). A meta-analysis published in 2014 identified an increased risk of NHL in workers exposed to glyphosate formulations. In March 2015 the World Health Organization's International Agency for Research on Cancer classified glyphosate as "probably carcinogenic in humans" (category 2A) based on epidemiological studies, animal studies, and in vitro studies. In November, 2015, the European Food Safety Authority published an updated assessment report on glyphosate, concluding that "the substance is unlikely to be genotoxic (i.e. damaging to DNA) or to pose a carcinogenic threat to humans." Furthermore, the final report clarified that while there may be other, probably carcinogenic, glyphosate-containing formulations, studies "that look solely at the active substance glyphosate do not show this effect."
- 1 Discovery
- 2 Chemistry
- 3 Mode of action
- 4 Environmental fate
- 5 Use
- 6 Formulations and tradenames
- 7 Toxicity
- 7.1 Glyphosate alone
- 7.2 Glyphosate-based formulations
- 7.3 Government and organization positions
- 8 Effects of use
- 9 Legal status
- 10 Legal cases
- 11 See also
- 12 References
- 13 External links
Glyphosate was first synthesized in 1950 by Swiss chemist Henry Martin, who worked for the Swiss company Cilag. The work was never published.:1 Stauffer Chemical patented the agent as a chemical chelator in 1964 as it binds and removes minerals such as calcium, magnesium, manganese, copper, and zinc.
Somewhat later, glyphosate was independently discovered at Monsanto in 1970. Monsanto chemists had synthesized about 100 analogs of aminomethylphosphonic acid as potential water-softening agents. Two were found to have weak herbicidal activity, and John E. Franz, a chemist at Monsanto, was asked to try to make analogs with stronger herbicidal activity. Glyphosate was the third analog he made.:1–2 Franz received the National Medal of Technology in 1987 and the Perkin Medal for Applied Chemistry in 1990 for his discoveries.
"Glyphosate resistance evolution is a major adverse development because glyphosate is a one in a 100-year discovery that is as important for reliable global food production as penicillin is for battling disease."— Stephen B. Powles PNAS 2010
Glyphosate is an aminophosphonic analogue of the natural amino acid glycine and like all amino acids exists in different ionic states depending on pH. Both the phosphonic acid and carboxylic acid moieties can be ionised and the amine group can be protonated and the substance exists as a series of zwitterions. Glyphosate is soluble in water to 12 g/L at room temperature. The original synthetic approach to glyphosate involved the reaction of phosphorus trichloride with formaldehyde followed by hydrolysis to yield a phosphonate. Glycine is then reacted with this phosphonate to yield glyphosate, and its name is taken as a contraction of the compounds used in this synthesis - viz. glycine and a phosphonate.
- PCl3 + H2CO → Cl2P(=O)-CH2Cl
- Cl2P(=O)-CH2Cl + 2 H2O → (HO)2P(=O)-CH2Cl + 2 HCl
- (HO)2P(=O)-CH2Cl + H2N-CH2-COOH → (HO)2P(=O)-CH2-NH-CH2-COOH + HCl
There are two main approaches used to synthesize glyphosate industrially. The first is to react iminodiacetic acid with phosphorous acid and hydrochloric acid (sometimes formed in situ by addition of phosphorus trichloride) via a modified Mannich reaction. Oxidation then leads to the desired glyphosate product. Iminodiacetic acid is usually prepared on-site, such as by reaction of chloroacetic acid with ammonia and calcium hydroxide to produce the calcium iminodiacetate salt and then acidification of the product.
The chloroacetic acid approach is less efficient than other iminodiacetic acid approaches, owing to the production of calcium chloride waste and decreased yield. When hydrogen cyanide is readily available as a by-product (say), an alternative approach is to use iminodiacetonitrile, HN(CH2CN)2, and diethanolamine is also a suitable starting material.
The second involves the use of dimethyl phosphite in a one-pot synthesis. Glycine and paraformaldehyde are reacted in a suitable organic solvent (typically triethylamine and methanol) to produce bishydroxymethylglycine, (HOCH2)2NCH2COOH. Dimethyl phosphite is then introduced and work-up with hydrochloric acid cleaves the hydroxymethyl group from the nitrogen atom whilst heating speeds the hydrolysis of both phosphate ester linkages.
This synthetic approach is responsible for a substantial portion of the production of glyphosate in China, with considerable work having gone into recycling the triethylamine and methanol. Progress has also been made in attempting to eliminate the need for triethylamine altogether.
Mode of action
Glyphosate kills plants by interfering with the synthesis of the aromatic amino acids phenylalanine, tyrosine, and tryptophan. It does this by inhibiting the enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS), which catalyzes the reaction of shikimate-3-phosphate (S3P) and phosphoenolpyruvate to form 5-enolpyruvyl-shikimate-3-phosphate (EPSP). Inhibiting the enzyme causes shikimate to accumulate in plant tissues and diverts energy and resources away from other processes. While growth stops within hours of application it takes several days for the leaves to start to turn yellow.
EPSP is subsequently dephosphorylated to chorismate, an essential precursor for the amino acids mentioned above. These amino acids are used in protein synthesis and to produce secondary metabolites such as folates, ubiquinones, and naphthoquinone.
X-ray crystallographic studies of glyphosate and EPSPS show that glyphosate functions by occupying the binding site of the phosphoenolpyruvate, mimicking an intermediate state of the ternary enzyme substrates complex. Glyphosate inhibits the EPSPS enzymes of different species of plants and microbes at different rates.
Glyphosate is absorbed through foliage and minimally through roots. Because of this mode of action, it is only effective on actively growing plants; it is not effective in preventing seeds from germinating.
Glyphosate adsorbs strongly to soil, and residues are expected to generally be immobile in soil. Ground and surface water pollution is limited. Glyphosate is readily degraded by soil microbes to aminomethylphosphonic acid (AMPA, which like glyphosate strongly adsorbs to soil solids and is thus unlikely to leach to groundwater).Though both glyphosate and AMPA are commonly detected in water bodies, a portion of the AMPA detected may actually be the result of degradation of detergents rather than from glyphosate. Glyphosate does have the potential to contaminate surface waters due to its aquatic use patterns and through erosion, as it adsorbs to soil particles suspended in runoff. The mechanism of glyphosate sorption to soil is similar to that of phosphate fertilizers, the presence of which can reduce glyphosate sorption. Phosphate fertilizers are subject to release from sediments into water bodies under anaerobic conditions, and similar release can also occur with glyphosate, though significant impact of glyphosate release from sediments has not been established. Limited leaching can occur after high rainfall after application. If glyphosate reaches surface water, it is not broken down readily by water or sunlight.
The half-life of glyphosate in soil ranges between 2 and 197 days; a typical field half-life of 47 days has been suggested. Soil and climate conditions affect glyphosate's persistence in soil. The median half-life of glyphosate in water varies from a few to 91 days. At a site in Texas, half-life was as little as three days. A site in Iowa had a half-life of 141 days. The glyphosate metabolite AMPA has been found in Swedish forest soils up to two years after a glyphosate application. In this case, the persistence of AMPA was attributed to the soil being frozen for most of the year. Glyphosate adsorption to soil, and later release from soil, varies depending on the kind of soil. A 2009 study using a RoundUp formulation concluded absorption into plants delays subsequent soil degradation and can increase glyphosate persistence in soil from two to six times.
According to the National Pesticide Information Center fact sheet, glyphosate is not included in compounds tested for by the Food and Drug Administration's Pesticide Residue Monitoring Program, nor in the United States Department of Agriculture's Pesticide Data Program. However, a field test showed that lettuce, carrots, and barley contained glyphosate residues up to one year after the soil was treated with 3.71 lb of glyphosate per acre (4.15 kg per hectare).
Glyphosate is effective in killing a wide variety of plants, including grasses and broadleaf and woody plants. By volume, it is one of the most widely used herbicides. In 2007, glyphosate was the most used herbicide in the United States agricultural sector, with 180 to 185 million pounds (82,000 to 84,000 tonnes) applied, the second-most used in home and garden with 5 to 8 million pounds (2,300 to 3,600 tonnes) and government applied 13 to 15 million pounds (5,900 to 6,800 tonnes) in industry and commerce. It is commonly used for agriculture, horticulture, viticulture, and silviculture purposes, as well as garden maintenance (including home use). It has a relatively small effect on some clover species and morning glory.
Glyphosate and related herbicides are often used in invasive species eradication and habitat restoration, especially to enhance native plant establishment in prairie ecosystems. The controlled application is usually combined with a selective herbicide and traditional methods of weed eradication such as mulching to achieve an optimal effect.
In many cities, glyphosate is sprayed along the sidewalks and streets, as well as crevices in between pavement where weeds often grow. However, up to 24% of glyphosate applied to hard surfaces can be run off by water. Glyphosate contamination of surface water is attributed to urban and agricultural use. Glyphosate is used to clear railroad tracks and get rid of unwanted aquatic vegetation. Since 1994, glyphosate has been used in aerial spraying in Colombia in coca eradication programs; Colombia announced in May 2015 that by October it would cease using glyphosate in these programs due to concerns about human toxicity of the chemical.
In addition to its use as an herbicide, glyphosate is also used for crop desiccation (siccation) to increase harvest yield and, as a result of desiccation, to increase sucrose concentration in sugarcane before harvest.
Genetically modified crops
Some micro-organisms have a version of 5-enolpyruvoyl-shikimate-3-phosphate synthetase (EPSPS) resistant to glyphosate inhibition. A version of the enzyme that was both resistant to glyphosate and that was still efficient enough to drive adequate plant growth was identified by Monsanto scientists after much trial and error in an Agrobacterium strain called CP4, which was found surviving in a waste-fed column at a glyphosate production facility.:56 This CP4 EPSPS gene was cloned and transfected into soybeans. In 1996, genetically modified soybeans were made commercially available. Current glyphosate-resistant crops include soy, maize (corn), canola, alfalfa, sugar beets, and cotton, with wheat still under development.
In 2015, 89% of corn, 94% of soybeans, and 89% of cotton produced in the US were genetically modified to be herbicide-tolerant.
Formulations and tradenames
Glyphosate is marketed in the United States and worldwide by many agrochemical companies, in different solution strengths and with various adjuvants, under dozens of tradenames. As of 2010, there were more than 750 glyophosate products on the market. In 2012, in terms of volume about half of the total global consumption of glyphosate was for conventional crops; Asia Pacific was the largest and fastest growing market. Chinese manufacturers collectively are the world's largest producers of glyphosate and its precursors and account for about 30% of global exports. Key manufacturers include Anhui Huaxing Chemical Industry Company, BASF, Bayer CropScience, Dow AgroSciences, DuPont, Jiangsu Good Harvest-Weien Agrochemical Company, Monsanto, Nantong Jiangshan Agrochemical & Chemicals Co., Nufarm Limited, SinoHarvest, Syngenta, and Zhejiang Xinan Chemical Industrial Group Company.
Glyphosate is an acid molecule, so it is formulated as a salt for packaging and handling. Various salt formulations include isopropylamine, diammonium, monoammonium, or potassium as the counterion. Some brands include more than one salt. Some companies report their product as acid equivalent (ae) of glyphosate acid, or some report it as active ingredient (ai) of glyphosate plus the salt, and others report both. To compare performance of different formulations, knowledge of how the products were formulated is needed. Given that different salts have different weights, the acid equivalent is a more accurate method of expressing and comparing concentrations.
Adjuvant loading refers to the amount of adjuvant already added to the glyphosate product. Fully loaded products contain all the necessary adjuvants, including surfactant; some contain no adjuvant system, while other products contain only a limited amount of adjuvant (minimal or partial loading) and additional surfactants must be added to the spray tank before application. As of 2000 (just before Monsanto's patent on glyphosate expired), over 400 commercial adjuvants from over 34 different companies were available for use in commercial agriculture.
Products are supplied most commonly in formulations of 120, 240, 360, 480, and 680 g/l of active ingredient. The most common formulation in agriculture is 360 g/l, either alone or with added cationic surfactants.
For 360 g/l formulations, European regulations allow applications of up to 12 l/ha for control of perennial weeds such as couch grass. More commonly, rates of 3 l/ha are practiced for control of annual weeds between crops.
Monsanto developed and patented the use of glyphosate to kill weeds in the early 1970s, and first brought it to market in 1974, under the Roundup brandname. While its initial patent expired in 1991, Monsanto retained exclusive rights in the United States until its patent on the isopropylamine salt expired in September 2000.
As of 2009, sales of these herbicide products represented about 10% of Monsanto's revenue due to competition from other producers of other glyphosate-based herbicides; their Roundup products (which include GM seeds) represented about half of Monsanto's gross margin.
Monsanto also produces seeds which grow into plants genetically engineered to be tolerant to glyphosate. The genes contained in these seeds are patented. Such crops allow farmers to use glyphosate as a postemergence herbicide against most broadleaf and cereal weeds. Soy was the first glyphosate-resistant crop.
Glyphosate is the active ingredient in herbicide formulations containing it. However, in addition to glyphosate salts, commercial formulations of glyphosate contain additives such as surfactants which vary in nature and concentration. The surfactants are added to enable the glyphosate to penetrate the cuticle of the plants. Toxicologists have studied glyphosate alone and formulations.
Many regulatory and scholarly reviews have evaluated the relative toxicity of glyphosate as an herbicide. The German Federal Institute for Risk Assessment toxicology review in 2013 found that "the available data is contradictory and far from being convincing" with regard to correlations between exposure to glyphosate formulations and risk of various cancers, including non-Hodgkin lymphoma (NHL).
Early epidemiological studies did not find associations between long-term low-level exposure to glyphosate and any disease. A 2000 review concluded that "under present and expected conditions of new use, there is no potential for Roundup herbicide to pose a health risk to humans". A 2002 review by the European Union reached the same conclusion. In 2013 the European commission reviewed a 2002 finding that had concluded equivocal evidence existed of a relationship between glyphosate exposure during pregnancy and cardiovascular malformations and found that "there is no increased risk at the levels of exposure below those that caused maternal toxicity." A 2013 review found that neither glyphosate nor typical glyphosate-based formulations (GBFs) pose a genotoxicity risk in humans under normal conditions of human or environmental exposures.
A 2014 review article reported a significant association between B-cell lymphoma and glyphosate occupational exposure. In March 2015 the World Health Organization's International Agency for Research on Cancer classified glyphosate as "probably carcinogenic in humans" (category 2A) based on epidemiological studies, animal studies, and in vitro studies. However in 2016 a joint meeting of the United Nations (FAO) Panel of Experts on Pesticide Residues in Food and the Environment and the World Health Organization (WHO) Core Assessment Group on Pesticide Residues (JMPR) concluded that based on the available evidence "glyphosate is unlikely to pose a carcinogenic risk to humans from exposure through the diet".
Amongst mammals, glyphosate is considered to have "low to very low toxicity". The LD50 of glyphosate is 5,000 mg/kg for rats, 10,000 mg/kg in mice and 3,530 mg/kg in goats. The acute dermal LD50 in rabbits is greater than 2,000 mg/kg. Indications of glyphosate toxicity in animals typically appear within 30 to 120 mins following ingestion of a large enough dose, and include initial excitability and tachycardia, ataxia, depression and bradycardia, although severe toxicity can develop into collapse and convulsions.
Glyphosate can have carcinogenic effects in non-human mammals. These include the induction of positive trends in the incidence of renal tubule carcinoma and haemangiosarcoma in male mice, and increased pancreatic islet-cell adenoma in male rats.
Glyphosate-based herbicides may cause life-threatening arrhythmias in mammals. There is also evidence that such herbicides cause direct electrophysiological changes in the cardiovascular systems of rats and rabbits.
Glyphosate is generally less persistent in water than in soil, with 12- to 60-day persistence observed in Canadian ponds, although persistence of over a year has been recorded in the sediments of American ponds. The half-life of glyphosate in water is between 12 days and 10 weeks.
For green frogs (Rana clamitans) glyphosate has a 24- and 96-hour LC50 of greater than 38.9 mg/L. A 2014 review found that amphibians have been identified as particularly sensitive to glyphosate formulations in their larval and tadpole stages of development, and toxicity has been extensively studied for those stages; toxicity for terrestrial life cycle stages is less well understood. The review found that "across the spectrum of organisms likely to be exposed to glyphosate in the aquatic environment, it has been shown that sensitivity to glyphosate (and the constituents of commercial formulas) is highly species-specific. A 2012 study on the toxic potential of environmentally relevant concentrations of glyphosate on wood frogs (Rana sylvatica), leopard frogs (Rana pipiens pipiens) and American toads (Bufo americanus) reported a significant induction of morphological changes in the tadpoles of the three species, including alteration of the size of the tadpole tail. This indicates that the herbicide could be affecting the mechanisms of development that are normally used as defence responses against predators. These results showed that glyphosate "can have widespread and relevant effects on non target species." Developmental toxicity appears to occur in the larvae and tadpoles of the American toad at levels of exposure to glyphosate that occur in common use of the herbicide.
Other aquatic fauna
In freshwater invertebrates (species unspecified), glyphosate has a 48-hour LC50 ranging from 55 to 780 ppm. The 96-hour LC50 is 281 ppm for grass shrimps (Palaemonetas vulgaris) and 934 ppm for fiddler crabs (Uca pagilator). These values make glyphosate "slightly toxic to practically non-toxic".
A study observing the impact of herbicides on the biodiversity of aquatic communities containing algae and more than 25 species of animals showed that in contrast to 2,4-D, glyphosate had great impact in the community, causing a decrease of 22% of the species richness.
The antimicrobial activity of glyphosate has been described in the microbiology literature since its discovery in 1970 and the description of glyphosate's mechanism of action in 1972. Efficacy was described for numerous bacteria and fungi. Glyphosate can control the growth of apicomplexan parasites, such as Toxoplasma gondii, Plasmodium falciparum (malaria) and Cryptosporidium parvum, and has been considered an antimicrobial agent in mammals. Inhibition can occur with some Rhizobium species important for soybean nitrogen fixation, especially under moisture stress.
When glyphosate comes into contact with the soil, it can be bound to soil particles, thereby slowing its degradation. In sewage sludge, unbound glyphosate has been degraded by bacteria.[relevant? ] Glyphosate and its degradation product, aminomethylphosphonic acid, residues are considered to be much more benign toxicologically and environmentally than most of the herbicides replaced by glyphosate.[relevant? ]
Glyphosate-based formulations (GBFs) may contain a number of adjuvants, the identities of which are considered trade secrets. In the United States, the Federal Insecticide, Fungicide, and Rodenticide Act requires that all pesticides (including herbicides) be evaluated by the EPA prior to sale. The data required for registration of an active ingredient (in this case, glyphosate) and its product formulations are dependent upon the use requested. This may include information on the active ingredient's chemistry, environmental fate, residue chemistry, dietary and non-dietary hazards to humans, and hazards to domestic animals and non-target organisms. Evaluations are performed for each active ingredient and for each product formulation. Additional evaluations also are performed by the EPA to set permitted residue levels in food for pesticide products used on food crops.
Surfactants are used in herbicide formulations as wetting agents, to maximize coverage and aid penetration of the herbicide(s) through plant leaves. As agricultural spray adjuvants, surfactants may be premixed in commercial formulations, such as Roundup, or they may be purchased separately and mixed on-site (tank mix).
Polyethoxylated tallow amine (POEA) is a surfactant used in the original Roundup formulation and was still being commonly used in 2015. Different versions of Roundup have included different percentages of POEA. Although Monsanto product fact sheets do not disclose surfactants and their percentages, a 1997 US government report said that Roundup is 15% POEA while Roundup Pro is 14.5%. A review of the literature provided to the EPA in 1997 found that POEA was more toxic to fish than glyphosate was. POEA is more toxic to fish and amphibians than glyphosate alone.
Spreader 90 is a surfactant used in tank mixes. Spreader 90 contains 1,2 propanediol (also known as propylene glycol), propane 1,2,3 triol (also known as glycerol), alcohol ethoxylate, and dimethylpolysiloxane. Of these ingredients, alcohol ethoxylates are among the widely used detergents in consumer products; commercial preparations are often mixes of homologs. Due to known toxicities to aquatic species, the Canadian Environmental Protection Act, 1999 recommended Federal Water Quality Guideline values of 70 µg/l.
A 2015 review of glyphosate-based herbicides (GlyBH) examined the toxic effects of GlyBH measured below regulatory limits found evidence that "GlyBH could be toxic below the regulatory lowest observed adverse effect level for chronic toxic effects," including teratogenic, tumorigenic and hepatorenal effects. The researchers concluded that their findings "challenge the assumption of safety of GlyBH at the levels at which they contaminate food and the environment, albeit these levels may fall below regulatory thresholds."
A 2000 review concluded that "under present and expected conditions of new use, there is no potential for Roundup herbicide to pose a health risk to humans". A 2002 review by the European Union reached the same conclusion.
Acute toxicity is dose-related; Skin exposure to ready-to-use glyphosate formulations can cause irritation, and photocontact dermatitis has been occasionally reported. These effects are probably due to the preservative benzisothiazolin-3-one. Severe skin burns are very rare. Inhalation is a minor route of exposure, but spray mist may cause oral or nasal discomfort, an unpleasant taste in the mouth, or tingling and irritation in the throat. Eye exposure may lead to mild conjunctivitis. Superficial corneal injury is possible if irrigation is delayed or inadequate. Death has been reported after deliberate overdose. Ingestion of Roundup ranging from 85 to 200 ml (of 41% solution) has resulted in death within hours of ingestion, although it has also been ingested in quantities as large as 500 ml with only mild or moderate symptoms. A reasonable correlation is seen between the amount of Roundup ingested and the likelihood of serious systemic sequelae or death. Ingestion of more than 85 ml of the concentrated formulation is likely to cause significant toxicity in adults. Corrosive effects – mouth, throat and epigastric pain and dysphagia – are common. Kidney and liver impairment are also frequent, and usually reflect reduced organ perfusion. Respiratory distress, impaired consciousness, pulmonary edema, infiltration on chest X-ray, shock, arrhythmias, renal failure requiring haemodialysis, metabolic acidosis, and hyperkalaemia may occur in severe cases. Bradycardia and ventricular arrhythmias often present prior to death.
A 2012 meta-analysis of all epidemiological studies of exposure to glyphosate formulations found no correlation with any kind of cancer. The 2013 systematic review by the German Institute for Risk Assessment of epidemiological studies of workers who use pesticides, exposed to glyphosate formulations found no significant risk, stating that "the available data is contradictory and far from being convincing".:Volume 1, p64-66 However, a 2014 meta-analysis of the same studies found a correlation between occupational exposure to glyphosate formulations and increased risk of B cell lymphoma, the most common kind of non-Hodgkin lymphoma (NHL). Workers exposed to glyphosate were about twice as likely to get B cell lymphoma.
A 2000 review of the ecotoxicological data on Roundup shows at least 58 studies exist on the effects of Roundup on a range of organisms. This review concluded, "...for terrestrial uses of Roundup minimal acute and chronic risk was predicted for potentially exposed non-target organisms".
In reproductive toxicity studies performed in rats and rabbits, no adverse maternal or offspring effects were seen at doses below 175–293 mg/kg of body weight per day.
Monsanto and other companies produce glyphosate products with alternative surfactants specifically formulated for aquatic use, for example the Monsanto products "Biactive" and "AquaMaster". Glyphosate formulations are much more toxic for amphibians and fish than glyphosate alone. The half-life of POEA (21–42 days) is longer than that for glyphosate (7–14 days) in aquatic environments.
The LC50 of formulations for Catla catla is 4.6 ppm, 4.2 ppm for rainbow trout (Oncorhynchus mykiss) and 1.3 ppm for bluegill (Lepomis macrochirus) The 96 h-LC50 toxicity of Roundup for the neotropical ray-finned fish, Prochilodus lineatus, is 13.69 mg/L, indicating this fish is more sensitive to Roundup than rainbow trout and Atlantic salmon (Salmo salar). Short-term exposure of Prochilodus lineatus to sub-lethal concentrations of Roundup results in biochemical, physiological and histological alterations. Histopathological changes are observed in the gills, livers and brains in other fishes at these concentrations.
Some researchers have suggested the toxicity effects of pesticides on amphibians may be different to those of other aquatic fauna because of their lifestyle; amphibians may be more susceptible to the toxic effects of pesticides because they often prefer to breed in shallow, lentic or ephemeral pools. These habitats do not necessarily constitute formal water-bodies and can contain higher concentrations of pesticide compared to larger water-bodies. Studies in a variety of amphibians have shown the toxicity of GBFs containing POEA to amphibian larvae. These effects include interference with gill morphology and mortality from either the loss of osmotic stability or asphyxiation. At sub-lethal concentrations, exposure to POEA or glyphosate/POEA formulations have been reported to be associated with delayed development, accelerated development, reduced size at metamorphosis, developmental malformations of the tail, mouth, eye and head, histological indications of intersex and symptoms of oxidative stress.
Gray tree frogs have been shown in selection trials to avoid pools contaminated with glyphosate/POEA and to prefer to lay their eggs in clean pools.
A 2003 study of various formulations of glyphosate found, "[the] risk assessments based on estimated and measured concentrations of glyphosate that would result from its use for the control of undesirable plants in wetlands and over-water situations showed that the risk to aquatic organisms is negligible or small at application rates less than 4 kg/ha and only slightly greater at application rates of 8 kg/ha." However, another perspective in the field is that amphibians are particularly susceptible to effects of POEA particularly because they often prefer breeding habitat of shallow, lentic, and ephemeral pools that often contain higher levels of POEA in agricultural lands. Serious sub-lethal effects on aquatic animals have been noted for formulations of glyphosate.
A 2013 meta-analysis reviewed the available data related to potential impacts of glyphosate-based herbicides on amphibians. According to the authors, the use of glyphosate-based pesticides cannot be considered the major cause of amphibian decline, the bulk of which occurred prior to the widespread use of glyphosate or in pristine tropical areas with minimal glyphosate exposure. The authors recommended further study of species- and development-stage chronic toxicity, of environmental glyphosate levels, and ongoing analysis of data relevant to determining what if any role glyphosate might be playing in worldwide amphibian decline, and suggest including amphibians in standardized test batteries.
In 2001, the Monsanto product Vision® was studied in a forest wetlands site in Canada. Substantial mortality occurred only at concentrations exceeding the expected environmental concentrations as calculated by Canadian regulatory authorities. While it was found that site factors such as pH and suspended sediments substantially affected the toxicity in the amphibian larvae tested, overall, "results suggest that the silvicultural use of Vision herbicide in accordance with the product label and standard Canadian environmental regulations should have negligible adverse effects on sensitive larval life stages of native amphibians."
Other aquatic fauna
The application rate of Roundup for areas in which the freshwater shrimp (Caridina nilotica) grows is considered to be 20–30 mg/L (acid equivalence). Shrimps exposed to varying concentrations (1.5–50 mg/L) showed neonates to be more sensitive to Roundup than both juveniles and adults, with mean 96 h LC50 values of 2.5, 7.0 and 25.3 mg/L respectively. All three life-stages of unexposed shrimp exhibited active and coordinated movements, however, shrimps exposed to Roundup were erratic and slow in their movements, with neonates showing most of these behavioral irregularities.
Effect on plant health
A correlation was found between an increase in the infection rate of wheat by Fusarium head blight and the application of glyphosate, but "because of the nature of this study, we could not determine if the association between previous GF (glyphosate formulation) use and FHB development was a cause-effect relationship". Other studies have found causal relationships between glyphosate and decreased disease resistance. Exposure to glyphosate has been shown to change the species composition of endophytic bacteria in plant hosts, which is highly variable.
In 2007, the EPA selected glyphosate for further screening through its Endocrine Disruptor Screening Program (EDSP). Selection for this program is based on a compound's prevalence of use and does not imply particular suspicion of endocrine activity. On June 29, 2015 the EPA released Weight of Evidence Conclusion of the EDSP Tier 1 screening for glyphosate, recommending that glyphosate not be considered for Tier 2 testing. The Weight of Evidence conclusion stated "...there was no convincing evidence of potential interaction with the estrogen, androgen or thyroid pathways."
Several studies have not found mutagenic effects, so glyphosate has not been listed in the United States Environmental Protection Agency or the International Agency for Research on Cancer databases. Various other studies suggest glyphosate may be mutagenic. Glyphosate-based formulations produce DNA strand breaks in several major taxa of animals including numerous fish species (European eel, sábalo, guppy, bloch, neotropical fish (Corydoras paleatus) carp, goldfish), reptiles (caiman), amphibians (frogs, including tadpoles) and gastropods (snails), but not in oysters, clams and mussel glochidia. In earthworms, one glyphosate-based formulation induced DNA strand breaks while two others did not, thereby emphasising the potential importance of components other than the active ingredients in the formulations.
Government and organization positions
A vote on reauthorization of glyphosate in the E.U. stalled in March 2016. Member states France, Sweden, and the Netherlands objected to the renewal. A vote to reauthorize on a temporary basis failed in June 2016.
European Food Safety Authority
A 2013 systematic review by the German Institute for Risk Assessment (BfR) examined more than 1000 epidemiological studies, animal studies, and in vitro studies. It found that "no classification and labelling for carcinogenicity is warranted" and did not recommend a carcinogen classification of either 1A or 1B.:139, 34–37 It provided the review to EFSA in January 2014 which published it in December 2014. On November, 12th, 2015, EFSA published its conclusion on the risk assessment of glyphosate, stating it was "unlikely to pose a carcinogenic hazard to humans".
EFSA's decision and the BfR report were criticized in an open letter published by 96 scientists in November 2015 saying that the BfR report failed to adhere to accepted scientific principles of open and transparent procedures. The BfR report included unpublished data, lacked authorship, omitted references, and did not disclose conflict-of-interest information.
On April 4, 2016, Dr. Vytenis Andriukaitis, European Commissioner for Health and Food Safety, wrote an open letter to the Chair of the Board of the Glyphosate Task at Monsanto Europe asking to publish the full studies provided to the EFSA.
US Environmental Protection Agency
The EPA, which last reviewed glyphosate in 1993, considers glyphosate to be noncarcinogenic and relatively low in dermal and oral acute toxicity. The EPA considered a "worst case" dietary risk model of an individual eating a lifetime of food derived entirely from glyphosate-sprayed fields with residues at their maximum levels. This model indicated that no adverse health effects would be expected under such conditions. As of March 2015, the EPA was in the midst of reviewing glyphosate's toxicity.
International Agency for Research on Cancer
In March 2015, the International Agency for Research on Cancer published a summary of their forthcoming monograph on glyphosate, and classified glyphosate as "probably carcinogenic in humans" (category 2A) based on epidemiological studies, animal studies, and in vitro studies. It noted that there was "limited evidence" of carcinogenicity in humans for non-Hodgkin lymphoma. The IARC classifies substances for their carcinogenic potential, and "a few positive findings can be enough to declare a hazard, even if there are negative studies as well." Unlike the BfR it does not conduct a so-called risk assessment weighing benefits against risk.
The BfR responded that IARC reviewed only a selection of what they had reviewed earlier, and argued that other studies, including a cohort study called 'Agricultural Health Study', do not support the classification. The IARC report did not include the German regulatory study published in December 2014, nor did it include industry-funded studies. Monsanto called the IARC report biased and said it wanted it to be retracted. It started a case against California's carcinogen classification in 2016.
Effects of use
In the 1990s when the first genetically modified crops-such as glyphosate-resistant corn, canola, soybean and cotton—were introduced, there were no glyphosate-resistant weeds. By 2014 glyphosate-resistant weeds dominated herbicide-resistant research. At that time there were 23 glyphosate-resistant species in eighteen countries.
"Resistance evolves after a weed population has been subjected to intense selection pressure in the form of repeated use of a single herbicide." Weeds resistant to the herbicide have been called 'superweeds'.
According to Ian Heap, a weed specialist, who completed his PhD on resistance to multiple herbicides in annual ryegrass (Lolium rigidum) in 1988 –the first case of an herbicide-resistant weed in Australia–by 2014 the Lolium rigidum was the "world’s worst herbicide-resistant weed with instances in "12 countries, 11 sites of action, 9 cropping regimes" and affecting over 2 million hectares. Annual ryegrass was known to be resistant to herbicides since 1982. By 1996 the first documented case of glyphosate-resistant Lolium rigidum was reported in Australia in 1996 near Orange, New South Wales. In 2006, farmers associations were reporting 107 biotypes of weeds within 63 weed species with herbicide resistance. In 2009, Canada identified its first resistant weed, giant ragweed, and at that time 15 weed species had been confirmed as resistant to glyphosate. As of 2010, in the United States 7 to 10 million acres (2.8 to 4.0 million hectares) of soil were afflicted by superweeds, or about 5% of the 170 million acres planted with corn, soybeans, and cotton, the crops most affected, in 22 states. In 2012, Charles Benbrook reported that the Weed Science Society of America listed 22 superweeds in the U.S., with over 5.7 million hectares (14 million acres) infested by GR weeds and that Dow AgroSciences had carried out a survey and reported a figure of around 40 million hectares (100 million acres). The International Survey of Herbicide Resistant Weeds database lists species that are resistant to glyphosate.
In response to resistant weeds, farmers are hand-weeding, using tractors to turn over soil between crops, and using other herbicides in addition to glyphosate.
In 2004, a glyphosate-resistant variation of Amaranthus palmeri, commonly known as Palmer amaranth, was found in Georgia and confirmed by a 2005 study. In 2005, resistance was also found in North Carolina. Widespread use of Roundup Ready crops led to an unprecedented selection pressure, and glyphosate resistance followed. The weed variation is now widespread in the southeastern United States. Cases have also been reported in Texas and Virginia.
Conyza bonariensis (also known as hairy fleabane and buva) and Conyza canadensis (known as horseweed or marestail), are other weed species that had lately developed glyphosate resistance. A 2008 study on the current situation of glyphosate resistance in South America concluded "resistance evolution followed intense glyphosate use" and the use of glyphosate-resistant soybean crops is a factor encouraging increases in glyphosate use. In the 2015 growing season, glyphosate-resistant marestail proved to be especially problematic to control in Nebraska production fields.
Glyphosate-resistant ryegrass (Lolium) has occurred in most of the Australian agricultural areas and other areas of the world. All cases of evolution of resistance to glyphosate in Australia were characterized by intensive use of the herbicide while no other effective weed control practices were used. Studies indicate the resistant ryegrass does not compete well against nonresistant plants and their numbers decrease when not grown under conditions of glyphosate application.
Use of glyphosate to clear milkweed along roads and fields may have contributed to a decline in monarch butterfly populations in the Midwest. Along with deforestation and adverse weather conditions, the decrease in milkweed contributed to an 81% decline in monarchs. The Natural Resources Defense Council (NRDC) filed a suit in 2015 against the EPA, in which it is argued that the agency ignored warnings about the dangers of glyphosate usage for monarchs.
Glyphosate was first approved for use in the 1970s, and as of 2010 was labelled for use in 130 countries.:2
In May 2015, Colombia announced that it would stop using glyphosate by October 2015 in the destruction of illegal plantations of coca, the raw ingredient for cocaine. Farmers have complained that the aerial fumigation has destroyed entire fields of coffee and other legal produce.
In June 2015, the French Ecology Minister asked nurseries and garden centers to halt over-the-counter sales of glyphosate in the form of Monsanto's Roundup. This was a nonbinding request and all sales of glyphosate remain legal in France until 2022, when the substance will be banned.
The New York Times reported that in 1996, "Dennis C. Vacco, the Attorney General of New York, ordered the company Monsanto to pull ads that said Roundup was "safer than table salt" and "practically nontoxic" to mammals, birds and fish. The company withdrew the spots, but also said that the phrase in question was permissible under E.P.A. guidelines."
In 2001, French environmental and consumer rights campaigners brought a case against Monsanto for misleading the public about the environmental impact of its herbicide Roundup, on the basis that glyphosate, Roundup's main component, is classed as "dangerous for the environment" and "toxic for aquatic organisms" by the European Union. Monsanto's advertising for Roundup had presented it as biodegradable and as leaving the soil clean after use. In 2007, Monsanto was convicted of false advertising and was fined 15,000 euros. Monsanto's French distributor Scotts France was also fined 15,000 euros. Both defendants were ordered to pay damages of 5,000 euros to the Brittany Water and Rivers Association and 3,000 euros to the CLCV (Consommation Logement Cadre de vie), one of the two main general consumer associations in France. Monsanto appealed and the court upheld the verdict; Monsanto appealed again to the French Supreme Court, and in 2009 it also upheld the verdict.
Trade dumping allegations
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