SKAP1

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SKAP1
Protein SKAP1 PDB 1u5d.png
Available structures
PDB Ortholog search: PDBe RCSB
Identifiers
Aliases SKAP1, HEL-S-81p, SCAP1, SKAP55, src kinase associated phosphoprotein 1
External IDs MGI: 1925723 HomoloGene: 2764 GeneCards: SKAP1
Genetically Related Diseases
ovarian cancer[1]
RNA expression pattern
PBB GE SKAP1 205790 at fs.png
More reference expression data
Orthologs
Species Human Mouse
Entrez
Ensembl
UniProt
RefSeq (mRNA)

NM_001075099
NM_003726

NM_001033186
NM_001177898
NM_001177899

RefSeq (protein)

NP_001068567
NP_003717

Location (UCSC) Chr 17: 48.13 – 48.43 Mb Chr 11: 96.46 – 96.76 Mb
PubMed search [2] [3]
Wikidata
View/Edit Human View/Edit Mouse

Src kinase-associated phosphoprotein 1 is an adapter protein that in humans is encoded by the SKAP1 gene.[4][5]

This gene encodes a T cell adapter protein, a class of intracellular molecules with modular domains capable of recruiting additional proteins but that exhibit no intrinsic enzymatic activity. The encoded protein contains a unique N-terminal region followed by a PH domain and C-terminal SH3 domain. Along with the adhesion and degranulation-promoting adapter protein, the encoded protein plays a critical role in inside-out signaling by coupling T-cell antigen receptor stimulation to the activation of integrins.[5]

The demonstration that SKAP1 regulates LFA-1 adhesion was made by retroviral infection [6] and by the SKAP1 deficient mouse.[7] Additional work has implicated SKAP1 in binding to the exchange factor RasGRP1 and in regulating ERK activation in T-cells.[8][9]

Interactions[edit]

SKAP1 has been shown to interact with FYN,[4][10][11] PTPRC[12] and FYB.[11][13]

References[edit]

  1. ^ "Diseases that are genetically associated with SKAP1 view/edit references on wikidata". 
  2. ^ "Human PubMed Reference:". 
  3. ^ "Mouse PubMed Reference:". 
  4. ^ a b Marie-Cardine A; Bruyns E; Eckerskorn C; Kirchgessner H; Meuer SC; Schraven B (July 1997). "Molecular cloning of SKAP55, a novel protein that associates with the protein tyrosine kinase p59fyn in human T-lymphocytes". J Biol Chem. 272 (26): 16077–80. doi:10.1074/jbc.272.26.16077. PMID 9195899. 
  5. ^ a b "Entrez Gene: SKAP1 src kinase associated phosphoprotein 1". 
  6. ^ Wang H, Moon EY, Azouz A, et al. (2003). "SKAP-55 regulates integrin adhesion and formation of T cell-APC conjugates". Nat. Immunol. 4 (4): 366–74. doi:10.1038/ni913. PMID 12652296. 
  7. ^ Wang H, Liu H, Lu Y, Lovatt M, Wei, B, Rudd CE Functional defects of SKAP-55 deficient T-cells identify a regulatory role for the adaptor in LFA-1 adhesion, Mol. Cell. Biol. 2007 27, 6863-75.
  8. ^ *Kosco KA, Cerignoli F, Williams S, et al. (2008). "SKAP55 modulates T cell antigen receptor-induced activation of the Ras-Erk-AP1 pathway by binding RasGRP1". Mol. Immunol. 45 (2): 510–22. doi:10.1016/j.molimm.2007.05.024. PMID 17658605. 
  9. ^ Schneider H., Wang H., Raab M., Valk E., Smith X. , Lovatt M., Wu Z., Strebhardt K, Rudd, CE. Adaptor SKAP55 binds RasGRP1 and negatively regulates p21ras and the ERK pathway in T-cells. (2008) PLoS ONE. 3, 1718.
  10. ^ Wu, Liangtang; Yu Zhenbao; Shen Shi-Hsiang (October 2002). "SKAP55 recruits to lipid rafts and positively mediates the MAPK pathway upon T cell receptor activation". J. Biol. Chem. United States. 277 (43): 40420–7. doi:10.1074/jbc.M206023200. ISSN 0021-9258. PMID 12171928. 
  11. ^ a b Marie-Cardine, A; Hendricks-Taylor L R; Boerth N J; Zhao H; Schraven B; Koretzky G A (October 1998). "Molecular interaction between the Fyn-associated protein SKAP55 and the SLP-76-associated phosphoprotein SLAP-130". J. Biol. Chem. UNITED STATES. 273 (40): 25789–95. doi:10.1074/jbc.273.40.25789. ISSN 0021-9258. PMID 9748251. 
  12. ^ Wu, Liangtang; Fu Jun; Shen Shi-Hsiang (April 2002). "SKAP55 coupled with CD45 positively regulates T-cell receptor-mediated gene transcription". Mol. Cell. Biol. United States. 22 (8): 2673–86. doi:10.1128/MCB.22.8.2673-2686.2002. ISSN 0270-7306. PMC 133720Freely accessible. PMID 11909961. 
  13. ^ Liu, J; Kang H; Raab M; da Silva A J; Kraeft S K; Rudd C E (July 1998). "FYB (FYN binding protein) serves as a binding partner for lymphoid protein and FYN kinase substrate SKAP55 and a SKAP55-related protein in T cells". Proc. Natl. Acad. Sci. U.S.A. UNITED STATES. 95 (15): 8779–84. doi:10.1073/pnas.95.15.8779. ISSN 0027-8424. PMC 21153Freely accessible. PMID 9671755. 

Further reading[edit]