Santa Fe Island (Spanish: Isla Santa Fe), also called Barrington Island after admiral Samuel Barrington, is a small island of 24 square kilometres (9.3 sq mi) which lies in the centre of the Galapagos archipelago, to the south east of Santa Cruz Island. Geologically it is one of the oldest, since volcanic rocks of about 4 million years old have been found. The vegetation of the island is characterized by brush, palo santo trees and stands of a large variety of the prickly pear cactus Opuntia echios.
Among animals, Santa Fe is home to one endemic species and one endemic subspecies: the Barrington land iguana (Conolophus pallidus) and the Santa Fe rice rat (Aegialomys galapagoensis bauri).
The visitor site is a wet landing located in Barrington Bay on the northeastern side of the island. Large numbers of sea lions are found on the beaches in the bay, occasionally hindering peregrination to the two trails leading from the beach.