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The Seidel Test is used to assess the presence of anterior chamber leakage in the cornea. It is used as a screening test for many corneal disorders including corneal post-trauma, corneal perforation and corneal degeneration.
Do not apply pressure on the eye during the test due to the risk of tissue extrusion.
A fluorescein strip containing 10% fluorescein is applied topically to the affected area and is examined with a cobalt blue filter. At this point, the fluorescein appears green in color. Any changes in color or surface of the fluorescence area indicate the presence of corneal leakage.
Results of the test
If the fluorescein strip turns pale upon application to the corneal surface, the person tests positive for the corneal deformity he/ she is being tested for. The change in the color of the fluorescein strip is due to dilution of fluorescein caused by the aqueous leakage in the cornea.